A novel selective growth medium-PCR assay to isolate and detect Sphingomonas in environmental samples

Yim, Mi-Sung; Yau, Yvonne; Matlow, Anne; So, Jae-Seong; Zou, Jitao; Flemming, Cecily A.; Schraft, Heidi; Leung, Ka Yin

doi:10.1016/j.mimet.2010.03.012

Cited by 43 publications

(31 citation statements)

References 42 publications

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“…Sphingomonadaceae are common inhabitants of drinking water worldwide (19,27,29,42) and have a remarkable capacity to cope with stress conditions and to adapt to new habitats (14,19,25,37,44). The current study provides the first evidence that these bacteria can play an important role as antibiotic resistance reservoirs in drinking water.…”

Section: Discussionmentioning

confidence: 68%

“…For instance, the ability to degrade xenobiotic compounds is one of the most remarkable properties of these bacteria (1,37). The capacity to survive in chlorinated waters, allegedly due to the oligotrophic character of these bacteria and their production of biofilms (14,19,25,44), is another demonstration of their plasticity in man-made environments. Sphingomonads are, thus, truly ubiquitous bacteria frequently found in aquatic environments, such as drinking water (bulk water and biofilms formed on pipes, reservoirs, and bathtubs), distilled water, hemodialysis fluids, or supposedly sterile drug solutions (14,17,24,25,31,34,38).…”

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confidence: 99%

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Diversity and Antibiotic Resistance Patterns of Sphingomonadaceae Isolates from Drinking Water

Vaz‐Moreira

Nunes

Manaia

2011

Appl Environ Microbiol

177

114

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Sphingomonadaceae (n ‫؍‬ 86) were isolated from a drinking water treatment plant (n ‫؍‬ 6), tap water (n ‫؍‬ 55), cup fillers for dental chairs (n ‫؍‬ 21), and a water demineralization filter (n ‫؍‬ 4). The bacterial isolates were identified based on analysis of the 16S rRNA gene sequence, and intraspecies variation was assessed on the basis of atpD gene sequence analysis. The isolates were identified as members of the genera Sphingomonas (n ‫؍‬ 27), Sphingobium (n ‫؍‬ 28), Novosphingobium (n ‫؍‬ 12), Sphingopyxis (n ‫؍‬ 7), and Blastomonas (n ‫؍‬ 12). The patterns of susceptibility to five classes of antibiotics were analyzed and compared for the different sites of isolation and taxonomic groups. Colistin resistance was observed to be intrinsic (92%). The highest antibiotic resistance prevalence values were observed in members of the genera Sphingomonas and Sphingobium and for beta-lactams, ciprofloxacin, and cotrimoxazole. In tap water and in water from dental chairs, antibiotic resistance was more prevalent than in the other samples, mainly due to the predominance of isolates of the genera Sphingomonas and Sphingobium. These two genera presented distinct patterns of association with antibiotic resistance, suggesting different paths of resistance development. Antibiotic resistance patterns were often related to the species rather than to the site or strain, suggesting the importance of vertical resistance transmission in these bacteria. This is the first study demonstrating that members of the family Sphingomonadaceae are potential reservoirs of antibiotic resistance in drinking water.

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Section: Discussionmentioning

confidence: 68%

mentioning

confidence: 99%

Diversity and Antibiotic Resistance Patterns of Sphingomonadaceae Isolates from Drinking Water

Vaz‐Moreira

Nunes

Manaia

2011

Appl Environ Microbiol

177

114

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“…More important, they can adapt to man-made environments. Most notably, these bacteria can degrade xenobiotic compounds (Stolz 2009) and can survive in chlorinated waters, possibly because of their oligotrophic character and production of biofilms (Furuhata et al 2007;Hong et al 2010;Yim et al 2010), which is further proof of their remarkable capacity to adapt to new habitats; hence, the dominance of Sphingomonadaceae in all treatment groups was not unexpected.…”

Section: Discussionmentioning

confidence: 81%

Impacts of different salinities on bacterial biofilm communities in fresh water

et al. 2014

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Natural and anthropogenic salinization continuously impacts inland aquatic ecosystems. Associated bacterial biofilms respond rapidly to environmental conditions and are potential bioindicators for changes in water quality. This study evaluates the effects of different salinity concentrations (0.3‰-10‰) on bacterial biofilms communities grown in fresh water from Lake Bosten. Bacterial communities associated with biofilms were analyzed using terminal restriction fragment length polymorphism and clone library analyses of 16S rRNA genes. Results indicated that the attached bacterial community composition (ABCC) changed over several weeks of biofilm growth, but all followed similar bacterial successional trends in the different salinity groups. Detailed analysis showed the following. (i) ABCC did not differ (P > 0.05) in the low-salinity groups (0.3‰-3.5‰), which may be related to the lower osmotic pressure and the shorter time scale (weeks) of their present habitats. (ii) There were significant differences between the oligosaline (3.5‰) and saline (10‰) groups (P < 0.05). In particular, genus Flavobacterium became dominant in attached bacterial communities in the saline groups. The higher abundance of genus Flavobacterium was possibly due to the biological and metabolic characteristics of the bacteria. (iii) Some bacterial taxa can maintain the higher abundance within attached bacteria in the entire process of biofilms growth, such as the genera Hydrogenophaga and Methyloversatilis in Betaproteobacteria and the family Sphingomonadaceae in Alphaproteobacteria. These data suggested that the bacterial successional trends within biofilms seem almost unaffected by salinity (0.3‰-10‰), but ABCC in saline groups (10‰) are notably changed.

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“…The former genus Sphingomonas has now been divided into five genera: Sphingomonas, Sphingobium, Novosphingobium, Sphingopyxis and Sphingosinicella (Takeuchi et al 2001;Maruyama et al 2006). Members of the genus Sphingomonas are Gram-negative, rod-shaped, strictly aerobic bacteria with high G + C content (White et al 1996;Yim et al 2010). The typical characteristics of the genus Sphingomonas include: Q-10 as the predominant respiratory quinone, sphingoglycolipid as the major polar lipid and sym-homospermidine as the major polyamine (Zhang et al 2010;Lin et al 2012;An et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Sphingomonas flavus sp. nov. isolated from road soil

Singh

Won

et al. 2015

Arch Microbiol

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A yellow-colored, Gram-negative, strictly aerobic, non-motile, rod-shaped bacterium, designated THG-MM5(T), was isolated from road soil in Yongin-si, Gyeonggi-do, Republic of Korea. Based on 16S rRNA gene sequence, strain THG-MM5(T) was moderately related to Sphingomonas sediminicola KACC 15039(T) (96.1%), Sphingomonas ginsengisoli KACC 16858(T) (96.1%) and Sphingomonas jaspsi KACC 13230(T) (96.0%). Chemotaxonomic data revealed that strain THG-MM5(T) possesses ubiquinone-10 as the only respiratory quinone, sym-homospermidine as the major polyamine and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C18:1 ω7c and C16:0 as the major fatty acids. The polar lipid profile included sphingoglycolipid. The DNA G + C content was 60.7 mol%. These data, together with phenotypic characterization, corroborated the affiliation of strain THG-MM5(T) to the genus Sphingomonas. Thus, the isolate represents a novel species, for which the name Sphingomonas flavus sp. nov. is proposed, with THG-MM5(T) as the type strain (=KACC 18277(T) = CCTCC AB 2014320(T)).

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A novel selective growth medium-PCR assay to isolate and detect Sphingomonas in environmental samples

Cited by 43 publications

References 42 publications

Diversity and Antibiotic Resistance Patterns of Sphingomonadaceae Isolates from Drinking Water

Diversity and Antibiotic Resistance Patterns of Sphingomonadaceae Isolates from Drinking Water

Impacts of different salinities on bacterial biofilm communities in fresh water

Sphingomonas flavus sp. nov. isolated from road soil

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