A Novel Scaffold from Recombinant Spider Silk Protein in Tissue Engineering

Wang, Hong Xin; Xue, Zheng; Wei, Mei; Chen, Deng Long; Li, Min

doi:10.4028/www.scientific.net/amr.152-153.1734

Cited by 5 publications

(8 citation statements)

References 20 publications

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“…The facilitation of endothelial cell growth was further demonstrated on pNSR16/Gt‐PU composite nanofibers, in agreement with previous studies using fibroblasts and endothelial . This was definited because gelatin and spider silk protein were both biocompatible materials being gift of cellular signal site, which can promote cell proliferation.…”

Section: Resultssupporting

confidence: 90%

“…Not only spider silk but also recombinant spider silk proteins are attracting more and more attention due to their potential mechanical property and perfect biocompatibility . Likewise, added with cell signaling amino acid sequence arginine‐glycin‐aspartic acid (RGD), a recombinant spider silk protein named pNSR16 has been successfully applied in small‐diameter vascular tissue engineering by our laboratory and finally identified its role in improving cell adhesion and proliferation . Compared with another natural protein, apart from common biological origin and biodegradability, gelatin (Gt) will make up for the hydrophobic defects of pNSR16 by virtue of its hydrophilic amino acids .…”

Section: Introductionmentioning

confidence: 99%

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A bilayered scaffold based on RGD recombinant spider silk proteins for small diameter tissue engineering

Zhang

Chen

et al. 2014

Polymer Composites

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A bilayered scaffold was fabricated via electrospinning layer-by-layer with the inner layer composed of blend fibers of spider silk protein and gelatin (pNSR16/Gt), then the polyurethane (PU) layer on the outside. The physicochemical and biological performance of scaffold were investigated. The pNSR16/Gt-PU composite nanofibers with interconnected pores was detected a porosity of 88.7 6 1.5%; with mechanical properties, including an appropriate permeability of 6.8 6 0.2 ml min 21 cm 22 , breaking strength (24.6 6 3.6 MPa) and elasticity up to 145 6 3.8% strain, and burst pressure reached 276 6 7.1 kPa as well as an accepted suture retention strength (4.9 6 0.8 N), optimized to mimic the nature artery. The pNSR16/Gt-PU bilayered scaffold also proved to be capable to support cell growth and proliferation of Sprague-Dawley rat aortic endothelial cells by 242 6 10% (7 days vs. control). After all, the intimal surface promotes endothelialization and being highly anti-thrombogenic, while the mismatch in mechanical properties outside induces intimal hyperplasia, it is desirable to develop a bioactive material with improved mechanical properties for smalldiameter vascular graft. POLYM. COMPOS., 37:523-531,

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Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

A bilayered scaffold based on RGD recombinant spider silk proteins for small diameter tissue engineering

Zhang

Chen

et al. 2014

Polymer Composites

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“…Foams remain one of the most ideal classes of material as they exhibit a high strength-to-weight ratio. Many research groups have demonstrated the use of recombinantly produced spidroins to manufacture highly porous and interconnected foam-like structures, used for example as 3D cell culture scaffolds. − Wang et al fabricated porous foams from a recombinantly produced spider silk protein called pNSR-16 containing the RGD motif cell-binding domain . The authors made the foams by dissolving the proteins with formic acid followed by the addition of NaCl.…”

Section: Bioengineering Of Protein-based Materialsmentioning

confidence: 99%

Protein-Based Biological Materials: Molecular Design and Artificial Production

Miserez

Mohammadi

2023

Chem. Rev.

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Polymeric materials produced from fossil fuels have been intimately linked to the development of industrial activities in the 20th century and, consequently, to the transformation of our way of living. While this has brought many benefits, the fabrication and disposal of these materials is bringing enormous sustainable challenges. Thus, materials that are produced in a more sustainable fashion and whose degradation products are harmless to the environment are urgently needed. Natural biopolymerswhich can compete with and sometimes surpass the performance of synthetic polymersprovide a great source of inspiration. They are made of natural chemicals, under benign environmental conditions, and their degradation products are harmless. Before these materials can be synthetically replicated, it is essential to elucidate their chemical design and biofabrication. For protein-based materials, this means obtaining the complete sequences of the proteinaceous building blocks, a task that historically took decades of research. Thus, we start this review with a historical perspective on early efforts to obtain the primary sequences of load-bearing proteins, followed by the latest developments in sequencing and proteomic technologies that have greatly accelerated sequencing of extracellular proteins. Next, four main classes of protein materials are presented, namely fibrous materials, bioelastomers exhibiting high reversible deformability, hard bulk materials, and biological adhesives. In each class, we focus on the design at the primary and secondary structure levels and discuss their interplays with the mechanical response. We finally discuss earlier and the latest research to artificially produce protein-based materials using biotechnology and synthetic biology, including current developments by start-up companies to scale-up the production of proteinaceous materials in an economically viable manner.

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“…Wang et al [58] reported production of porous foams made of the recombinant spider silk protein pNSR-16. This recombinant spider silk protein also comprises an RGD motif.…”

Section: Porous Foamsmentioning

confidence: 99%

Spider Silk for Tissue Engineering Applications

2020

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Due to its properties, such as biodegradability, low density, excellent biocompatibility and unique mechanics, spider silk has been used as a natural biomaterial for a myriad of applications. First clinical applications of spider silk as suture material go back to the 18th century. Nowadays, since natural production using spiders is limited due to problems with farming spiders, recombinant production of spider silk proteins seems to be the best way to produce material in sufficient quantities. The availability of recombinantly produced spider silk proteins, as well as their good processability has opened the path towards modern biomedical applications. Here, we highlight the research on spider silk-based materials in the field of tissue engineering and summarize various two-dimensional (2D) and three-dimensional (3D) scaffolds made of spider silk. Finally, different applications of spider silk-based materials are reviewed in the field of tissue engineering in vitro and in vivo.Molecules 2020, 25, 737 2 of 20 ampullate spidroins as it lacks polyalanine and glycine-proline-glycine motifs typically present in MaSp1 and MaSp2. It was shown that MaSp3 repetitive regions contain larger and more polar amino acids than that in MaSp1 or MaSp2 [9] Most spidroins consist of a repetitive core domain flanked by nonrepetitive amino-and carboxyl-terminal domains [10,11] The major ampullate spidroins assemble and form distinguishable substructures, which finally result in a hierarchically structured fiber. This fiber is then in some cases surrounded by glycoproteins and lipids [12].

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A Novel Scaffold from Recombinant Spider Silk Protein in Tissue Engineering

Cited by 5 publications

References 20 publications

A bilayered scaffold based on RGD recombinant spider silk proteins for small diameter tissue engineering

A bilayered scaffold based on RGD recombinant spider silk proteins for small diameter tissue engineering

Protein-Based Biological Materials: Molecular Design and Artificial Production

Spider Silk for Tissue Engineering Applications

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