A bilayered scaffold based on RGD recombinant spider silk proteins for small diameter tissue engineering

Abstract

A bilayered scaffold was fabricated via electrospinning layer-by-layer with the inner layer composed of blend fibers of spider silk protein and gelatin (pNSR16/Gt), then the polyurethane (PU) layer on the outside. The physicochemical and biological performance of scaffold were investigated. The pNSR16/Gt-PU composite nanofibers with interconnected pores was detected a porosity of 88.7 6 1.5%; with mechanical properties, including an appropriate permeability of 6.8 6 0.2 ml min 21 cm 22 , breaking strength (24.6 6 3.6 MPa) and elasticity up to 145 6 3.8% strain, and burst pressure reached 276 6 7.1 kPa as well as an accepted suture retention strength (4.9 6 0.8 N), optimized to mimic the nature artery. The pNSR16/Gt-PU bilayered scaffold also proved to be capable to support cell growth and proliferation of Sprague-Dawley rat aortic endothelial cells by 242 6 10% (7 days vs. control). After all, the intimal surface promotes endothelialization and being highly anti-thrombogenic, while the mismatch in mechanical properties outside induces intimal hyperplasia, it is desirable to develop a bioactive material with improved mechanical properties for smalldiameter vascular graft. POLYM. COMPOS., 37:523-531,

Electrospinning of ultrafine non‐hydrolyzed silk sericin/PEO fibers on PLA: A bilayer scaffold fabrication

“…[13][14][15][16] For biomedical applications and tissue engineering, the electrospun non-woven nanofibrous mimics the extracellular matrix much more efficiently compared to conventional techniques. [2][3][4][5] Nanoscale fiber can also be used as a drug delivery system. Dissolving the drug of interest in a polymer solution followed by the electrospinning of the mixture leads to drug loaded fibers.…”

“…Electrospinning is a versatile technique able to produce films made of entangled fibers with wide range of diameters from micro to nanometer scale. [1] This nanometric fiber size presents many interests for different fields of applications such as tissue engineering, [2][3][4][5] energy, [6,7] healthcare, [8,9] protective clothing, [10,11] filtration, [12] and so on. This versatility comes from the wide range of different polymers that can be electrospun, with controlled size and shape which are tunable for the desired applications.…”