Silkworm and spider silk electrospinning: a review

Belbéoch, Clémence; Lejeune, Joseph; Vroman, Philippe; Salaün, Fabien

doi:10.1007/s10311-020-01147-x

Cited by 78 publications

(53 citation statements)

References 195 publications

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“…To obtain a functional gene set, we removed the genes with an expression level of less than 0.1 and unannotated genes. Finally, 18,822,45,304,16,899, and 17,127 functional protein-coding gene sets were obtained for T. clavata, T. clavipes, T. inaurata madagascariensis, and N. pilipes, respectively (Table 1). BUSCO (v4.0.5) was used to determine the quality of our functional gene set using the eukaryote lineage, and the completeness scores were 91.4%, 87.5%, 91.0%, and 93.4% for the eukaryote lineage (Table 1).…”

Section: Draft Genomes Of Four Nephilinae Spidersmentioning

confidence: 99%

“…The difficulties are multifactorial; the unusually large protein size and the repetitive nature of its sequence are inherent challenges for synthesis, and the natural conditions of spinning are only beginning to be fully uncovered (13)(14)(15). However, one primary reason that full reproduction has not been successful is that the previous recombinant approaches employed only MaSp1, only MaSp2, or at most a combination of the two (11,(16)(17)(18). In fact, it is often suggested that dragline silk is composed primarily of two components, MaSp1 and MaSp2 (5,(19)(20)(21)(22)(23).…”

Section: Main Text Introductionmentioning

confidence: 99%

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Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk

Kono

Nakamura

Mori

et al. 2021

Preprint

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Dragline silk of golden orb-weaver spiders (Nephilinae) is noted for its unsurpassed toughness, combining extraordinary extensibility and tensile strength, suggesting industrial application as a sustainable biopolymer material. To pinpoint the molecular composition of dragline silk and the roles of its constituents in achieving its mechanical properties, we report a multiomics approach combining high-quality genome sequencing and assembly, silk gland transcriptomics, and dragline silk proteomics of four Nephilinae spiders. We observed the consistent presence of the MaSp3B spidroin unique to this subfamily, as well as several non-spidroin SpiCE proteins. Artificial synthesis and combination of these components in vitro showed that the multicomponent nature of dragline silk, including MaSp3B and SpiCE, along with MaSp1 and MaSp2, is essential to realize the mechanical properties of spider dragline silk.

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Section: Draft Genomes Of Four Nephilinae Spidersmentioning

confidence: 99%

Section: Main Text Introductionmentioning

confidence: 99%

Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk

Kono

Nakamura

Mori

et al. 2021

Preprint

View full text Add to dashboard Cite

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“…Electrospinning is widely used in tissue engineering and usually has good compatibility with biological tissues. Unlike the weaving method, electrospinning can produce ultrathin nonwoven textile with a large specific surface [ 56 , 57 ] and even mimic the histological structures through electrospinning process design [ 58 ]. Limited by the yield of natural spidroin, the recombinant spidroin produced by genetically engineered bacteria (e.g., Escherichia coli ) or yeasts (e.g., Pichia pastoris ) is adopted as raw material [ 59 ].…”

Section: Spidroin-based Biomaterials In Different Dimensionsmentioning

confidence: 99%

“…The corrosive, irritant, and environmentally harmful solvents (e.g., HFIP [ 28 , 64 ], GdmSCN [ 74 , 122 ], and formic acid [ 63 ]) are frequently adopted to dissolve recombinant spidroins for subsequent procedures due to their excellent ability to anti-self-assembly of spidroins, which definitely increases the operational risk and environmental issues. Fortunately, there is a new trend that uses gentle and environmentally friendly aqueous solvent when preparing a spidroin-based film or scaffold used for tissue engineering [ 57 ], such as Tris-HCl, SDS, and PEO400. Correspondingly, increasing the protein concentration within these solvents becomes a new challenge.…”

Section: Conclusion and Perspectivementioning

confidence: 99%

Spidroin-Based Biomaterials in Tissue Engineering: General Approaches and Potential Stem Cell Therapies

Zhang

Wang

et al. 2021

Stem Cells International

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Spider silks are increasingly gaining interest for potential use as biomaterials in tissue engineering and biomedical applications. Owing to their facile and versatile processability in native and regenerated forms, they can be easily tuned via chemical synthesis or recombinant technologies to address specific issues required for applications. In the past few decades, native spider silk and recombinant silk materials have been explored for a wide range of applications due to their superior strength, toughness, and elasticity as well as biocompatibility, biodegradation, and nonimmunogenicity. Herein, we present an overview of the recent advances in spider silk protein that fabricate biomaterials for tissue engineering and regenerative medicine. Beginning with a brief description of biological and mechanical properties of spidroin-based materials and the cellular regulatory mechanism, this review summarizes various types of spidroin-based biomaterials from genetically engineered spider silks and their prospects for specific biomedical applications (e.g., lung tissue engineering, vascularization, bone and cartilage regeneration, and peripheral nerve repair), and finally, we prospected the development direction and manufacturing technology of building more refined and customized spidroin-based protein scaffolds.

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“…More precisely, silk fibroin (SF) derived from Bombyx mori silk fibers was chosen as the structural support due to its advantages in sustainability, versatile processing, and tunable physical properties. [ 19–22 ] Ca 2+ and graphene were included in the system to enhance flame retardancy and provide possibilities for detection related to fire. This combination of components is sensitive to changes in temperature due to fire.…”

Section: Introductionmentioning

confidence: 99%

Electro‐Blown Spun Silk/Graphene Nanoionotronic Skin for Multifunctional Fire Protection and Alarm

et al. 2021

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Artificial protective skins are widely used in artificial intelligence robots, such as humanoid robots, mobile manipulation robots, and automatic probe robots, but their safety in use, especially flame retardancy, is rarely considered. As many artificial skins are designed for use in flammable or even explosive environments, flammability is a significant concern. Herein, a flame‐retardant silk/graphene nanoionotronic (SGNI) skin is developed by using a rationally designed high‐throughput electro‐blown spinning technique, with a more efficient production efficiency than electrospinning. These flame retardant SGNI skins combine the advantages of nanofibrous and ionotronic materials, and they are sustainable, conductive, highly porous, mechanically robust, highly stretchable, self‐adhesive, and humidity‐ and temperature‐sensitive. These merits support the assembly of SGNI skins into a fire alarm system, with real‐time alarm (response in 2 s) to mobile phones, clouds, and a central control system. The concept that combines a flame retardant and fire alarm material into an intelligent skin may provide potential solutions toward the design of protective skins for robotics and human–machine interactions.

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Silkworm and spider silk electrospinning: a review

Cited by 78 publications

References 195 publications

Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk

Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk

Spidroin-Based Biomaterials in Tissue Engineering: General Approaches and Potential Stem Cell Therapies

Electro‐Blown Spun Silk/Graphene Nanoionotronic Skin for Multifunctional Fire Protection and Alarm

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