A Novel Saliva RT-LAMP Workflow for Rapid Identification of COVID-19 Cases and Restraining Viral Spread

Kobayashi, Gerson Shigeru; Brito, Luciano Abreu; Moreira, Danielle de Paula; Suzuki, Adriano K.; Hsia, Gabriella Shih Ping; Pimentel, Lylyan Fragoso; Paiva, Ana Paula Barreto de; Dias, Carolina Regoli; Lourenço, Naila Cristina Vilaça; Oliveira, Beatriz Araujo; Manuli, Erika R.; Corral, Marcelo Andreetta; Cavaçana, Natale; Mitne‐Neto, Miguel; Sales, Marcelo Augusto Oliveira de; Aquila, Luiz Phellipe Dell; Filho, Alvaro Razuk; Parrillo, Eduardo Fagundes; Mendes-Corrêa, Maria Cássia; Sabino, Éster Cerdeira; Costa, Sílvia Figueiredo; Leal, Fábio E.; Sgro, Germán G.; Farah, Chuck S.; Zatz, Mayana; Passos‐Bueno, Maria Rita

doi:10.3390/diagnostics11081400

Cited by 22 publications

(23 citation statements)

References 45 publications

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“…Compared to nasopharyngeal RT-qPCR only, the RT-LAMP showed a weaker specificity and PPV, reflecting the negative individuals misclassified by NP RT-qPCR but also detected positive by salivary RT-qPCR. Further, viral load in NPS samples was weakly associated to RT-LAMP sensitivity as there was no correlation between salivary and NP RT-qPCR Ct values (Spearman correlation = 0.19), suggesting different saliva and NPS viral loads, corroborating previous works 6 , 40 .…”

Section: Discussionsupporting

confidence: 85%

Performances of rapid and connected salivary RT-LAMP diagnostic test for SARS-CoV-2 infection in ambulatory screening

Schneider

Molina

Picot

et al. 2022

Sci Rep

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In the context of social events reopening and economic relaunch, sanitary surveillance of SARS-CoV-2 infection is still required. Here, we evaluated the diagnostic performances of a rapid, extraction-free and connected reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay on saliva. Nasopharyngeal (NP) swabs and saliva from 443 outpatients were collected simultaneously and tested by reverse-transcription quantitative PCR (RT-qPCR) as reference standard test. Seventy-one individuals (16.0%) were positive by NP and/or salivary RT-qPCR. Sensitivity and specificity of salivary RT-LAMP were 85.9% (95%CI 77.8–94.0%) and 99.5% (98.7–100%), respectively. Performances were similar for symptomatic and asymptomatic participants. Moreover, SARS-CoV-2 genetic variants were analyzed and no dominant mutation in RT-LAMP primer region was observed during the period of the study. We demonstrated that this RT-LAMP test on self-collected saliva is reliable for SARS-CoV-2 detection. This simple connected test with optional automatic results transfer to health authorities is unique and opens the way to secure professional and social events in actual context of economics restart.

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Section: Discussionsupporting

confidence: 85%

Performances of rapid and connected salivary RT-LAMP diagnostic test for SARS-CoV-2 infection in ambulatory screening

Schneider

Molina

Picot

et al. 2022

Sci Rep

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“…Interestingly, in a recent study of our group [ 43 ] it was observed that, although there were no observed gender differences in viral load in nasopharyngeal samples, adult males showed a significantly higher viral load in saliva samples (verified by RT-LAMP viral testing) than adult women.…”

Section: Discussionmentioning

confidence: 71%

Men are the main COVID-19 transmitters: behavior or biology?

et al. 2022

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Background COVID-19 has affected millions of people worldwide. Clinical manifestations range from severe cases with lethal outcome to mild or asymptomatic cases. Although the proportion of infected individuals does not differ between sexes, men are more susceptible to severe COVID-19, with a higher risk of death than women. Also, men are pointed out as more lax regarding protective measures, mask wearing and vaccination. Thus, we questioned whether sex-bias may be explained by biological pathways and/or behavioral aspects or both. Methods Between July 2020 and July 2021, we performed an epidemiological survey including 1744 unvaccinated adult Brazilian couples, with there was at least one infected symptomatic member, who were living together during the COVID-19 infection without protective measures. Presence or absence of infection was confirmed by RT-PCR and/or serology results. Couples were divided into two groups: (1) both partners were infected (concordant couples) and (2) one partner was infected and the spouse remained asymptomatic despite the close contact with the COVID-19 symptomatic partner (discordant couples). Statistical analysis of the collected data was performed aiming to verify a differential transmission potential between genders in couples keeping contact without protective measures. Results The combination of our collected data showed that the man is the first (or the only) affected member in most cases when compared to women and that this difference may be explained by biological and behavioral factors. Conclusions The present study confirmed the existence of gender differences not only for susceptibility to infection and resistance to COVID-19 but also in its transmission rate.

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“…These data support the conclusion that LAMP is currently the most suitable iNAAT for SARS-CoV-2 diagnosis. With the recent demonstrations of using alternative specimens, including saliva [ 154 , 155 ], and exhaled breath samples (by a face mask-based collector) [ 156 ], RT-LAMP has been transformed to adapt better to the POC diagnostic settings.…”

Section: Isothermal Nucleic Acid Amplification Testing (Inaat) Methodsmentioning

confidence: 99%

Recent findings and applications of biomedical engineering for COVID-19 diagnosis: a critical review

et al. 2021

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COVID-19 is one of the most severe global health crises that humanity has ever faced. Researchers have restlessly focused on developing solutions for monitoring and tracing the viral culprit, SARS-CoV-2, as vital steps to break the chain of infection. Even though biomedical engineering (BME) is considered a rising field of medical sciences, it has demonstrated its pivotal role in nurturing the maturation of COVID-19 diagnostic technologies. Within a very short period of time, BME research applied to COVID-19 diagnosis has advanced with ever-increasing knowledge and

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A Novel Saliva RT-LAMP Workflow for Rapid Identification of COVID-19 Cases and Restraining Viral Spread

Cited by 22 publications

References 45 publications

Performances of rapid and connected salivary RT-LAMP diagnostic test for SARS-CoV-2 infection in ambulatory screening

Performances of rapid and connected salivary RT-LAMP diagnostic test for SARS-CoV-2 infection in ambulatory screening

Men are the main COVID-19 transmitters: behavior or biology?

Recent findings and applications of biomedical engineering for COVID-19 diagnosis: a critical review

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