A Novel RP-HPLC Method Development and Validation for the Determination of Pioglitazone and Glimepiride in Bulk and Pharmaceutical Formulations

Rao, B. Venkateswara; Vijetha, P.; Vidyadhara, S.; Kavitha, K

doi:10.5958/2231-5675.2017.00023.0

Cited by 5 publications

(1 citation statement)

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“…The chromatographic methods proposed for the determination of domperidone in the presence of other analytes (Mondal et al, 2011 and Rao et al, 2015) at acidic pH show that the basic analyte, domperidone, eluted earlier regardless of its hydrophobicity. This is because of the ionization effect of the domperidone in an acidic environment.…”

Section: Influence Of Ionization On Retention Behaviourmentioning

confidence: 99%

Retention behaviour of analytes in reversed‐phase high‐performance liquid chromatography—A review

V.¹,

Basuri²,

Sahini³

et al. 2022

Biomedical Chromatography

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The understanding of principles that drive the separation in reversed‐phase chromatography plays an important role in the prediction of the elution of solutes in RP‐HPLC. The separation in RP‐HPLC is based on the principle of adsorption and partition. In addition, the logP value, the pKa value of the drug and chromatographic parameters like mobile phase pH, buffer concentration, organic modifier and mobile phase additives also influence the retention and selectivity of the analyte. It was found that hydrophobic, electrostatic, hydrogen bonding and other specific interactions between the stationary phase and the solutes, along with the hydrophobicity of an analyte molecule (logP), modify the retention behaviour of the analytes. This article gives special attention to the influence of ionization and ion interaction on the separation of analytes. The drug molecules with different logP values containing protonated and deprotonated acids, bases and zwitterions are selected as examples and this article addresses various issues related to the method development, relationships between analyte retention and mobile phase pH and the pKa value of the analyte. The advances in this regard, with highlights on topics such as mechanisms of retention and various factors that influence the retention behaviour of analytes, are also updated with suitable examples.

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Section: Influence Of Ionization On Retention Behaviourmentioning

confidence: 99%

Retention behaviour of analytes in reversed‐phase high‐performance liquid chromatography—A review

V.¹,

Basuri²,

Sahini³

et al. 2022

Biomedical Chromatography

View full text Add to dashboard Cite

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Stability indicating LC-MS Method development and Validation for Quantitative analysis of Pimozide

Gorripati

Chintakula

Battula

et al. 2022

RJPT

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The objective of the current investigation is to study the degradation behaviour of the Pimozide under different stress conditions using LC-MS method. Pimozide is a diphenyl butylpiperidine derivative used as antipsychotic drug. It was exposed to different stress conditions like acidic, alkali, thermal, peroxide and UV light to investigate its stability. Successful separation of drug from its degradation products was achieved on Waters C18 column (150mm X 4.5mm, 5µm) using methanol and acetonitrile 65:35 (V/V) having pH 5.5 as mobile phase in isocratic elution mode at a flow rate of 1.0ml/min with UV detection at 230nm. Quantification was achieved using an electrospray ion interface operating in positive mode, under multiple reaction monitoring conditions. Optimized LC-MS conditions were validated by ICH Q2 (R1) guidelines with respect to specificity, precision, linearity, recovery, LOD and LOQ. The method proved that Pimozide was found sensitive under UV light and alkali conditions, stable under thermal stress condition and moderate stability under acidic and oxidation condition with lowest degradation. The developed LC-MS method was found simple, sensitive and efficiently separated the drug and its degradation products in all samples.

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A new Validated RP-HPLC Method for simultaneous quantification of Raloxifene and Curcumin

Tagde¹,

Nagpal²,

Kulkarni³

2022

RJPT

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Raloxifene (RLX) and Curcumin (CUR) are used for the treatment of breast cancer. Its combination is chosen because of their synergistic therapeutic potential, which helps to reduce drug-related toxicities while increasing therapeutic efficacy. A simple, sensitive, accurate and specific technique for determining retention time by employing reverse-phase high-performance liquid chromatography for simultaneous estimation of both the drugs was developed as well validated for the analysis on their particular wavelength. The separation was performed on an RP C-18 Column (EC 250/4 neucleosil 100-10) with column dimensions 250mm x 4.60mm, 10mM using mobile phase Potassium dihydrogen phosphate: acetonitrile (pH 3.0 with Orthophosphoric acid) in the ratio of 20:80 v/v and flow rate of 1.0min/mL. The specificity, linearity range, accuracy, sensitivity, robustness, precision, and ruggedness of the method were all determined. The analyte's peaks were found to be well resolved, and two different RTs for RLX and CUR were observed. Over a concentration range of 1-5g/ml, RLX and CUR calibration curves were shown to be linear. All of the technique validation criteria were within acceptable limits. For inter- and intra-day precision, the relative standard deviation (%RSD) was found to be <2%.

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A Novel RP-HPLC Method Development and Validation for the Determination of Pioglitazone and Glimepiride in Bulk and Pharmaceutical Formulations

Cited by 5 publications

References 0 publications

Retention behaviour of analytes in reversed‐phase high‐performance liquid chromatography—A review

Retention behaviour of analytes in reversed‐phase high‐performance liquid chromatography—A review

Stability indicating LC-MS Method development and Validation for Quantitative analysis of Pimozide

A new Validated RP-HPLC Method for simultaneous quantification of Raloxifene and Curcumin

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