A novel role for non-muscle γ-actin in skeletal muscle sarcomere assembly

Lloyd, Catriona; Berendse, M.; Lloyd, David G.; Schevzov, Galina; Grounds, Miranda D.

doi:10.1016/j.yexcr.2004.02.012

Cited by 22 publications

(31 citation statements)

References 29 publications

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“…Myotubes grown with the 2DMS technique were organized in parallel and separated from each other ( Figure 2C). Interestingly, bundles of F-actin fused into thicker assemblies and often the staining intensity displayed regular variations, suggesting that their amount or packing of F-actin was altered into a repetitive pattern along the bundle ( Figure 2D), in agreement with previous observations of a sarcomerelike arrangement in developing myotubes (Lloyd et al 2004;Sanger et al 2005).…”

Section: Subcellular and Nuclear Organization During Formation Of Ordsupporting

confidence: 91%

“…The 2DMS technique may thus prove advantageous to elucidate molecular mechanisms associated with nuclear cluster- (red) shows the presence of MyoD1 and MyoD2 nuclei in myoblasts which also were stained with FITC-phalloidin (F-actin; green) and DAPI (nuclei; blue). The cells were cultured for 1 day (Lloyd et al 2004). ing, perhaps related to initial stages of the formation of the neuromuscular junctions.…”

Section: Discussionmentioning

confidence: 99%

“…One example is the force-generating actin filament system, which is essential for motility and maintenance of cell polarity and undergoes extensive rearrangement on myotube formation. The successive reorganization of the actomyosin system and the shift in expression of the components of this contractile machinery from non-muscle to muscle isoforms exemplifies important and not fully understood processes during muscle development (Lloyd et al 2004;Sanger et al 2005;Hotulainen and Lappalainen 2006).…”

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confidence: 99%

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Myotube Formation on Micro-patterned Glass: Intracellular Organization and Protein Distribution in C2C12 Skeletal Muscle Cells

Yamamoto

Csikasz

Liu

et al. 2008

J Histochem Cytochem.

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S U M M A R Y Proliferation and fusion of myoblasts are needed for the generation and repair of multinucleated skeletal muscle fibers in vivo. Studies of myocyte differentiation, cell fusion, and muscle repair are limited by an appropriate in vitro muscle cell culture system. We developed a novel cell culture technique [two-dimensional muscle syncytia (2DMS) technique] that results in formation of myotubes, organized in parallel much like the arrangement in muscle tissue. This technique is based on UV lithography-produced micropatterned glass on which conventionally cultured C2C12 myoblasts proliferate, align, and fuse to neatly arranged contractile myotubes in parallel arrays. Combining this technique with fluorescent microscopy, we observed alignment of actin filament bundles and a perinuclear distribution of glucose transporter 4 after myotube formation. Newly formed myotubes contained adjacently located MyoD-positive and MyoD-negative nuclei, suggesting fusion of MyoD-positive and MyoD-negative cells. In comparison, the closely related myogenic factor Myf5 did not exhibit this pattern of distribution. Furthermore, cytoplasmic patches of MyoD colocalized with bundles of filamentous actin near myotube nuclei. At later stages of differentiation, all nuclei in the myotubes were MyoD negative. The 2DMS system is thus a useful tool for studies on muscle alignment, differentiation, fusion, and subcellular protein localization. (J Histochem Cytochem 56:881-892, 2008)

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Section: Subcellular and Nuclear Organization During Formation Of Ordsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Myotube Formation on Micro-patterned Glass: Intracellular Organization and Protein Distribution in C2C12 Skeletal Muscle Cells

Yamamoto

Csikasz

Liu

et al. 2008

J Histochem Cytochem.

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“…15,22,23 In muscle tissue, ACTG has been shown to have a role in sarcomere assembly. 24 Tissue-specific inactivation of Actg in mice does not interfere with embryonic development of the muscles, but mice show muscle weakness with a progressive pattern of muscle fiber necrosis and regeneration, indicating that Actg must have a crucial function in the adult skeletal muscle. In the muscle cell lineage, ACTB expression is downregulated during muscle maturation, in contrast to ACTG1, which remains localized in the sarcolemma of mature skeletal muscle.…”

Section: Baraitser-winter Cerebrofrontofacial Syndromementioning

confidence: 99%

Baraitser–Winter cerebrofrontofacial syndrome: delineation of the spectrum in 42 cases

Verloes¹,

Donato²,

et al. 2014

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Baraitser-Winter, Fryns-Aftimos and cerebrofrontofacial syndrome types 1 and 3 have recently been associated with heterozygous gain-of-function mutations in one of the two ubiquitous cytoplasmic actin-encoding genes ACTB and ACTG1 that encode b-and c-actins. We present detailed phenotypic descriptions and neuroimaging on 36 patients analyzed by our group and six cases from the literature with a molecularly proven actinopathy (9 ACTG1 and 33 ACTB). The major clinical anomalies are striking dysmorphic facial features with hypertelorism, broad nose with large tip and prominent root, congenital non-myopathic ptosis, ridged metopic suture and arched eyebrows. Iris or retinal coloboma is present in many cases, as is sensorineural deafness. Cleft lip and palate, hallux duplex, congenital heart defects and renal tract anomalies are seen in some cases. Microcephaly may develop with time. Nearly all patients with ACTG1 mutations, and around 60% of those with ACTB mutations have some degree of pachygyria with anteroposterior severity gradient, rarely lissencephaly or neuronal heterotopia. Reduction of shoulder girdle muscle bulk and progressive joint stiffness is common. Early muscular involvement, occasionally with congenital arthrogryposis, may be present. Progressive, severe dystonia was seen in one family. Intellectual disability and epilepsy are variable in severity and largely correlate with CNS anomalies. One patient developed acute lymphocytic leukemia, and another a cutaneous lymphoma, indicating that actinopathies may be cancerpredisposing disorders. Considering the multifaceted role of actins in cell physiology, we hypothesize that some clinical manifestations may be partially mutation specific. Baraitser-Winter cerebrofrontofacial syndrome is our suggested designation for this clinical entity.

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“…1a and 1b, [Kilpinen et al, 2008]). Despite their names and originally described expression pattern, these two actin isoforms are also expressed in undifferentiated muscle cells and, to a very limited extent, in differentiated muscle cells [Owens and Thompson, 1986;Rybakova et al, 2000;Lloyd et al, 2004;Hanft et al, 2006].…”

Section: Introductionmentioning

confidence: 99%

Actin isoform expression patterns during mammalian development and in pathology: Insights from mouse models

Tondeleir

Vandamme

Vandekerckhove

et al. 2009

Cell Motil. Cytoskeleton

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The dynamic actin cytoskeleton, consisting of six actin isoforms in mammals and a variety of actin binding proteins is essential for all developmental processes and for the viability of the adult organism. Actin isoform specific functions have been proposed for muscle contraction, cell migration, endo-and exocytosis and maintaining cell shape. However, these specific functions for each of the actin isoforms during development are not well understood. Based on transgenic mouse models, we will discuss the expression patterns of the six conventional actin isoforms in mammals during development and adult life. Ablation of actin genes usually leads to lethality and affects expression of other actin isoforms at the cell or tissue level. A good knowledge of their expression and functions will contribute to fully understand severe phenotypes or diseases caused by mutations in actin isoforms. Cell Motil. Cytoskeleton 66: 798-815, 2009. '

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A novel role for non-muscle γ-actin in skeletal muscle sarcomere assembly

Cited by 22 publications

References 29 publications

Myotube Formation on Micro-patterned Glass: Intracellular Organization and Protein Distribution in C2C12 Skeletal Muscle Cells

Myotube Formation on Micro-patterned Glass: Intracellular Organization and Protein Distribution in C2C12 Skeletal Muscle Cells

Baraitser–Winter cerebrofrontofacial syndrome: delineation of the spectrum in 42 cases

Actin isoform expression patterns during mammalian development and in pathology: Insights from mouse models

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