A Novel Rice PR10 Protein, RSOsPR10, Specifically Induced in Roots by Biotic and Abiotic Stresses, Possibly via the Jasmonic Acid Signaling Pathway

Abstract

Plant roots have important roles not only in absorption of water and nutrients, but also in stress tolerance such as desiccation, salt, and low temperature. We have investigated stress-response proteins from rice roots using 2-dimensional polyacrylamide-gel electrophoresis and found a rice protein, RO-292, which was induced specifically in roots when 2-week-old rice seedlings were subjected to salt and drought stress. The full-length RO-292 cDNA was cloned, and was determined to encode a protein of 160 amino acid residues (16.9 kDa, pI 4.74). The deduced amino acid sequence showed high similarity to known rice PR10 proteins, OsPR10a/PBZ1 and OsPR10b. RO-292 mRNA accumulated rapidly upon drought, NaCl, jasmonic acid and probenazole, but not by exposure to low temperature or by abscisic acid and salicylic acid. The RO-292 gene was also up-regulated by infection with rice blast fungus. Interestingly, induction was observed almost exclusively in roots, thus we named the gene RSOsPR10 (root specific rice PR10). The present results indicate that RSOsPR10 is a novel rice PR10 protein, which is rapidly induced in roots by salt, drought stresses and blast fungus infection possibly through activation of the jasmonic acid signaling pathway, but not the abscisic acid and salicylic acid signaling pathway.

Proteomics to identify pathogenesis-related proteins in rice roots under water deficit

“…4). Taken together, we strongly suggest that these three PR proteins may play roles in root development under water deficit other than disease resistance response (Kim et al 2001(Kim et al , 2008Hashimoto et al 2004). Concerning the induction mechanisms of PR proteins expression, especially in relation to the crosstalk between various biotic and abiotic stresses, there were only a few reports (Kim et al 2008).…”

“…RSOsPR10 exhibited a significant increase in Nipponbare root after 15 h air-drying treatment, and its transcription was strongly up-regulated within 3 h (Hashimoto et al 2004). JIOsPR10 was rapidly induced by air-dried treatment in the stem, but not in the leaves (Kim et al 2008), while its expression change in rice root was not examined.…”

“…The major families of PR proteins have been grouped at least into 14 different classes, primarily on the basis of their amino acid sequence identity (Hashimoto et al 2004). PR-1 was the first identified and the most dominant group among the PR proteins, and PR-1a was the acidic-type subclass of it (Kim et al 2001).…”

“…PR10 proteins are small, primarily acid intracellular proteins of about 16 kDa. The gene RSOsPR10 was rapidly induced almost exclusively in roots (named root specific rice PR10) by salt, desiccation and jasmonic acid (JA) (Hashimoto et al 2004). JIOsPR10 belongs to a different type of PR10 protein, which was also shown to be induced by JA and SA at the mRNA level , and up-regulated by some abiotic stresses at protein level (Kim et al 2008).…”

Research on the Rice Proteome: The Contribution of Proteomics Technology in the Creation of Abiotic Stress-Tolerant Plants

“…Hashimoto et al [19] investigated stress-response proteins from rice roots using 2D-PAGE and found a rice protein named as RSPR10 induced specifically in roots when 2-week-old rice seedlings were subjected to salt and drought stresses. The full-length cDNA for this protein was cloned and the deduced amino-acid sequence showed a high similarity to the known sequences of PR10, PR10a/ PBZ1, and PR10b.…”