A novel reaction-based fluorescent probe for the detection of cysteine in milk and water samples

Wang, Jialin; Wang, Hao; Hao, Yanfeng; Yang, Shaoxiang; Tian, Hongyu; Sun, Baoguo; Liu, Yongguo

doi:10.1016/j.foodchem.2018.04.084

Cited by 67 publications

(22 citation statements)

References 38 publications

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“…Advanced testing methods should be explored to obtain a lower detection limit. [40][41][42][43][44] Distribution of PHA in various fresh kidney bean cultivars PHA concentrations in fresh kidney bean cultivars were determined by hemagglutinating activity assays (Figure 1a). The standard curve is shown in (Figure 1b).…”

Section: Establish Of Pha Analysis Methodsmentioning

confidence: 99%

Phytohemagglutinin content in fresh kidney bean in China

Sun

Liu

Huang

et al. 2019

International Journal of Food Properties

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Fresh kidney beans (Phaseolus vulgaris L.) are common green vegetable that are widely cultured and consumed worldwide. The benefits of fresh kidney beans are highly substantial, but at the same time, toxicity caused by phytohemagglutinin (PHA) has raised concerns. PHA from fresh kidney beans accounted for 40% of poisoning events induced by poisonous plants in China from 2004 to 2013. However, an understanding of PHA levels in fresh kidney beans remains elusive. An analysis method for PHA based on agglutination of PHA to erythrocyte cell was established. We investigated the effect of cultivar, bean part, maturity, and cooking method on PHA content in fresh kidney beans. We demonstrated significant differences in the distribution of PHA among various predominant, fresh Chinese kidney bean cultivars and parts. One major Chinese strain, Zihuayoudou, was found to contain the highest amount of PHA, which was abundant in fresh kidney bean seeds. Moreover, PHA concentrations were found to decrease with the maturity of the fresh product. Further, cooking methods had a significant effect on PHA levels in fresh kidney beans. PHA could be effectively removed when stir-frying for more than 18 min, or braising for more than 10 min. These findings provide insight into the PHA content of fresh kidney beans in China and will be helpful to guide food safety measures.

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Section: Establish Of Pha Analysis Methodsmentioning

confidence: 99%

Phytohemagglutinin content in fresh kidney bean in China

Sun

Liu

Huang

et al. 2019

International Journal of Food Properties

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“…Canu et al ( 24 ) have shown that in human gastric cancer cells, miR-204 can reduce the Bcl-2 gene expression, enhance the therapeutic effect of 5-fluorouracil and promote apoptosis. Sirt1 is a highly-conserved deacetylase dependent on NAD + , which can regulate cell proliferation, stress responses and DNA damage ( 25 ). According to the study of Yuan et al ( 26 ), Sirt1 is one of the downstream target genes of miR-204 in human gastric cancer cells, and miR-204 inhibits the invasion and metastasis of gastric cancer cells through downregulating the Sirt1 expression, thus exerting an antitumor effect.…”

Section: Discussionmentioning

confidence: 99%

Expression of miR‑204 in pediatric retinoblastoma and its effects on proliferation and apoptosis of cancer cells

Ding

2018

Oncol Lett

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Expression, clinical significance and molecular mechanism of miR-204 in human retinoblastoma (RB) and para-carcinoma tissues were investigated. A total of 110 cases of RB tissues preserved after ophthalmectomy in the First Affiliated Hospital of Hunan Normal University (People's Hospital of Hunan Province) from April 2013 to June 2017 were collected along with 100 cases of para-carcinoma normal tissues. The expression of miR-204 in RB tissues was detected via reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and its associations with clinicopathological features were analyzed. Y79 cells were transfected with miR-204 mimics. A total of 80 pmol/l miR-204 mimics and 10 µl Lipofectamine 2000 were added into the experimental group. Cell proliferation was detected via methyl thiazolyl tetrazolium (MTT) assay at 24, 48, 72 and 96 h, apoptosis was detected via flow cytometry at 48 h after transfection, and the relative expression levels of B-cell lymphoma 2 (Bcl-2) messenger RNA (mRNA) and Sirt1 mRNA were detected via RT-qPCR. The results of MTT assay revealed that the measured value of the optical density (OD) in the experimental group at 48 h was obviously lower than that in the negative control group (p<0.001). The proportion of apoptotic cells in the experimental group was remarkably higher than that in the negative control group (p<0.001). Compared with those in the negative control group, the relative expression levels of Bcl-2 and Sirt1 mRNAs in the experimental group were significantly decreased (p<0.001). miR-204 may be involved in the occurrence and development of RB, which is significantly associated with clinical tissue differentiation, neural infiltration and lymph node metastasis in patients. miR-204 may inhibit proliferation and promote apoptosis of RB cells through downregulating the expression of Bcl-2 and Sirt1 in RB. Therefore, miR-204 may become a new biological index for early diagnosis, prognosis evaluation and biotherapy of RB.

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“…Yang et al appended a 2,4-dinitrobenzenesulfonyl moiety to fluorescein and applied the synthetic probe ( Fig. 1 , panel 2 ) to the detection of Cys in milk and water samples [137] . The probe proved to be highly selective and could be used to detect Cys concentrations in the range 0–400 μM.…”

Section: Fluorescent Probes For Rss and Rses Based On S N mentioning

confidence: 99%