A novel ratiometric electrochemical biosensor for sensitive detection of ascorbic acid

Wang, Li; Gong, Coucong; Shen, Yuan; Ye, Wenhui; Xu, Mengli; Song, Yonghai

doi:10.1016/j.snb.2016.11.100

Cited by 71 publications

(20 citation statements)

References 40 publications

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“…The ratio of the two signals is used to replace the absolute value of signal of analyte as the output signal, thus the reproducibility and robustness of the sensor can be remarkably enhanced. Nowadays, ratiometric electrochemical sensors have been developed for a varieties of biomolecules including nucleic acid, 22,23 biological small molecule, 24,25 protein, 26,27 and metal ions, 28,29 etc. But most ratiometric electrochemical sensors only can detect one analyte.…”

Section: Introductionmentioning

confidence: 99%

A multifunctional ratiometric electrochemical sensor for combined determination of indole-3-acetic acid and salicylic acid

Wang

et al. 2020

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

A multifunctional ratiometric electrochemical sensor for combined determination of indole-3-acetic acid and salicylic acid

Wang

et al. 2020

RSC Adv.

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“…The LOD was calculated based on the ratio between three times the standard deviation of the blank and the slope of the analytical curve. In comparison with the data reported in the literature, the achieved LOD is lower than the value found by Thiagarajan et al 36 and higher than the LODs found by other authors, including da Silva et al, 37 Kalimuthu et al, 38 Qi et al, 39 Zheng et al, 40 Wang et al, 41 Zhang et al, 42 and Taei et al 43 It is important to highlight that all mentioned reports explored modified electrodes for detecting AA. On the other hand, the proposed ePADs were used without any modification, making the electrode fabrication simpler, easier and cheaper than previous studies.…”

Section: Detection Of Ascorbic Acid By Square Wave Voltammetrymentioning

confidence: 50%

Determination of Ascorbic Acid in Commercial Tablets Using Pencil Drawn Electrochemical Paper-based Analytical Devices

et al. 2018

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This study describes the use of electrochemical paper-based analytical devices (ePADs) drawn with graphite pencil for the determination of ascorbic acid (AA) in commercial tablets. ePADs were fabricated using vegetal paper and graphite pencil. First, the three-electrode electrochemical cell drawn using a graphical software and toner lines were laser printed on the vegetal paper surface to delimit the electrode areas. Then, the electrode regions were manually painted with graphite pencil. Afterwards, the pseudo-reference electrode was defined with the deposition of silver ink over the graphite surface. Cyclic voltammetry and square wave voltammetry (SWV) experiments were performed to optimize the electroanalytical parameters as well as to quantitatively determine the AA concentration in two commercial tables. ePADs exhibited linear behavior for a concentration range between 0.5 and 3.0 mmol L. The achieved limit of detection and sensitivity were 70 μmol L and 0.47 μA/mmol L, respectively. The AA concentration levels found by SWV experiments in both Cenevit and Energil C were 2.80 ± 0.02 and 3.10 ± 0.01 mmol L, respectively. The accuracy of the proposed devices was investigated through recovery experiments in three concentration levels and it presented values between 95 and 115%.

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“…The detection limit was as low as 10 nM, which is superior to most of the previously reported AA sensor (see Table S1 in Supporting Information). ,− ,− The present AA RECS demonstrated a low working potential, wide linear range, and high sensitivity. Such a dynamic range toward AA detection well covered the physiological AA concentration of cerebral microdialysate (2–12 μM). , …”

Section: Results and Discussionmentioning

confidence: 79%

“…The ratiometric response could be obtained by the built-in reference and the analyte, which was able to overcome the system errors of the conventional ECS derived from the alteration of the environment and operating personnel. − With this strategy, the RECS was endowed with enhanced reproducibility, robustness, and reliability, which is a highly desirable method applicable for in vivo repetitive analysis. To date, the research on RECS for AA detection was still in its infancy, and only three reports have been published. − In this work, metal–organic frameworks capsulated Ketjen black, thionine/Ketjen black nanocomposites, and a multiwalled carbon nanotube fiber were employed to facilitate AA oxidation, but with inadequate sensitivity to detect the AA in brain microdialysate. Herein, we demonstrated a rational constructed RECS for selective, sensitive, and reliable detection of cerebral AA in the brain microdialysate (Scheme ).…”

mentioning

confidence: 99%

Facile Ratiometric Electrochemical Sensor for In Vivo/Online Repetitive Measurements of Cerebral Ascorbic Acid in Brain Microdiaysate

Jiang

Xiao

et al. 2020

Anal. Chem.

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The in vivo monitoring of ascorbic acid (AA) following physiological and pathological events is of great importance because AA plays a critical role in brain functions. The conventional electrochemical sensors (ECSs) usually suffered from poor selectivity and sluggish electron transfer kinetics for cerebral AA oxidation. The exploitation of ECSs adapt to the electrochemical detection (ECD)–microdialysis system, here we reported a facile ratiometric electrochemical sensor (RECS) for in vivo/online repetitive measurements of cerebral AA in brain microdiaysate. The sensor were constructed by careful electrodeposition of graphene oxide (GO) onto glassy carbon (GC) electrodes. Methylene blue (MB) was electrostatically adsorbed onto the GO surface as a built-in reference to achieve ratiometric detection of AA. The subsequent proper electroreduction treatment was able to readily facilitate the oxidation of AA at a relatively negative potential (−100 mV) and the oxidation of MB at separated potential (−428 mV). The in vitro experiments demonstrated that the RECS exhibited high sensitivity (detection limit: 10 nM), selectivity, and stability toward AA determination, enabling the in vivo/online repetitive measurement of cerebral AA in brain microdiaysate with high reliability. As a result, the designed RECS was successfully applied in the ECD–microdialysis system to in vivo/online repetitive monitoring the dynamic change of cerebral AA in the progress of the global cerebral ischemia/reperfusion events. More, the microinjection of endogenous AA and AA oxidase (AAOx) verified the reliability of the proposed RECS for in vivo/online repetitive cerebral AA detection. This proposed sensor filled the gap that no rational electrochemical sensor has been developed for the ECD–microdialysis system since its creation by the Mao group in 2005, which provided a reliable and effective method for brain chemistry research.

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A novel ratiometric electrochemical biosensor for sensitive detection of ascorbic acid

Cited by 71 publications

References 40 publications

A multifunctional ratiometric electrochemical sensor for combined determination of indole-3-acetic acid and salicylic acid

A multifunctional ratiometric electrochemical sensor for combined determination of indole-3-acetic acid and salicylic acid

Determination of Ascorbic Acid in Commercial Tablets Using Pencil Drawn Electrochemical Paper-based Analytical Devices

Facile Ratiometric Electrochemical Sensor for In Vivo/Online Repetitive Measurements of Cerebral Ascorbic Acid in Brain Microdiaysate

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