A novel, rapid, single-step immunochromatographic procedure for the detection of mouse immunoglobulin

Horton, Jeffrey K.; Swinburne, S.; O’Sullivan, Michael

doi:10.1016/0022-1759(91)90135-3

Cited by 40 publications

(23 citation statements)

References 2 publications

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“…Immunochromatographic assays were first described in the late 1960s and were originally developed to assess the presence of serum proteins (47). Other early assays that used an immunochromatographic technique include those for the quantification of drugs in biological fluids (100), theophylline in whole blood (57), and mouse immunoglobulin (36). Over the past decade many immunochromatographic assays have been reported for the detection of infectious diseases (2-4, 8, 9, 13, 15-17, 21, 22, 27, 29, 37, 42, 59-61, 69, 74-76, 84, 94), cancer (35,72), cardiovascular problems (54,65,66,73), pancreatitis (43), and illicit drugs (7,95).…”

Section: Immunochromatographic Lateral Flow Assaysmentioning

confidence: 99%

“…Lateral flow assays are run, as described above, washed in phosphate-buffered saline and a Tween 20 solution, and then immersed in a silver enhancer reagent for 5 min. Horton and coworkers reported sensitivities of 100 ng/ml before enhancement and 100 pg/ml after enhancement (36). This system has the advantage of greater sensitivity but does not need a specialized mechanism to read the assay, which is advantageous for use in a field setting.…”

Section: Immunochromatographic Lateral Flow Assaysmentioning

confidence: 99%

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Immunological Methods for Detection and Identification of Infectious Disease and Biological Warfare Agents

Peruski

2003

Clin Vaccine Immunol

195

119

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NATURE OF THE PROBLEMBW agents. The release of a biological weapon (BW) agent by a terrorist group or military force would likely be silent and undetectable or nearly so. As shown by anthrax attack during the fall of 2001 in the eastern United States, patients would begin appearing at hospitals and clinics within several days of exposure, most presenting with nonspecific flu-like symptoms. The first days of the outbreak might not even cause undue concern. However, depending on the type of agent and the method of dispersal, the public healthcare system would rapidly be stretched to capacity and beyond.The qualities that make a good BW agent are its relationship between aerosolization, infectivity, or toxicity and the amount of agent required to produce an effect (48). In addition, criteria such as environmental stability, ease of production, disease severity, and communicability determine which agents are the most likely to be utilized. For maximum effect, an optimal agent should be highly lethal and easily produced in large quantities and have limited options for preventive or prophylactic treatment. Given that the respiratory route is the most effective for most BW agents, stability in an aerosol form and the capability to be readily dispersed also in an aerosol (1-to 10-m particle size) are necessary. When potential agents are reviewed for these characteristics, Bacillus anthracis (anthrax) and variola major virus (smallpox) are considered to have the greatest potential for mass casualties and civil disruption. Also high on a prospective list of agents are botulinum neurotoxins, Yersinia pestis, and Francisella tularensis (48,91,92). Lower on the prospective list are Burkholderia pseudomallei and Burkholderia mallei, Rickettsia sp., Coxiella burnetii, Venezuelan equine encephalitis virus, Marburg and Ebola viruses, and influenza viruses (48,63,91,92).Emerging infectious disease agents. In addition to diseases caused by intentional epidemics, there are several emerging infectious diseases (ID) with the potential for significant public health consequences, including dengue fever, West Nile fever, and Rift Valley fever as well as the recent reemergence of malaria in the eastern United States (48,63,91,92). As with BW agents, emerging ID agents may be directly transmissible or vector borne (63). A complex interplay of factors can influence disease emergence, including genetic variation, environmental changes, and population pressures. Further compounding this already complicated situation, are the estimated 600 million international tourists annually, many with the potential to the spread disease globally in a matter of hours (63). Clearly, the challenges facing modern clinical microbiologists and immunologists are daunting enough without the added difficulties posed by the intentional release of BW/ID agents! Because of the threat posed by both BW and emerging or reemerging ID agents, there is a need to rapidly identify such agents in the clinical setting in order to treat the individuals at risk and to improve p...

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Section: Immunochromatographic Lateral Flow Assaysmentioning

confidence: 99%

Section: Immunochromatographic Lateral Flow Assaysmentioning

confidence: 99%

Immunological Methods for Detection and Identification of Infectious Disease and Biological Warfare Agents

Peruski

2003

Clin Vaccine Immunol

195

119

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“…At present, liquid chromatography tandem mass spectrometry (LC/MS/ MS), which provides a universal detector, has been successfully applied to the analysis of ASs in various biological samples and is used as a confirmatory method because of the high specificity of the information on the molecular structure of the analyte (Rapp and Meyer, 1989;Gaillard et al, 2000;Hewitt et al, 2002;Van Poucke and Peteghem, 2002). However, these tests are all tedious and time-consuming, and require a laboratory equipped with proper instruments and trained personnel (Horton et al, 1991;John et al, 2001;Yu et al, 2005). There is thus still a need to develop a novel, rapid, simple assay for detecting 19-NT on a large scale.…”

Section: Introductionmentioning

confidence: 98%

Development and evaluation of a rapid lateral flow immunochromatographic strip assay for screening 19‐nortestosterone

Liu

Peng

Jin

et al. 2007

Biomedical Chromatography

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The lateral flow strip test for 19-nortestosterone is one kind of immunochromatographic assay. Nitrocellulose membrane was separately immobilized with goat anti-rabbit IgG (control line) and 19-NT-OVA conjugate (test line). Anti-19-NT polyclonal antibody labeled with colloidal gold particles acted as the detector reagent. The assay is qualitatively, not quantitatively, judged with positive or negative result. We tested the sensitivity of the strip using spiked swine urine, and each specimen was independently measured by LC/MS/MS. The sensitivity, measure by eye, was determined to be 200 ng/mL. The assay time was less than 15 min, and so suitable for on-site rapid test.

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“…Silver enhancement of the response of the gold nanoparticles has been mentioned as well for sensitivity increase [89][90][91]. Apart from losing the "one-step" concept when using an enzyme, a biological element with limited stability is introduced; shelf life may decrease and handling becomes more complicated.…”

Section: Weaknessesmentioning

confidence: 99%

Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

2008

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Lateral flow (immuno)assays are currently used for qualitative, semiquantitative and to some extent quantitative monitoring in resource-poor or non-laboratory environments. Applications include tests on pathogens, drugs, hormones and metabolites in biomedical, phytosanitary, veterinary, feed/food and environmental settings. We describe principles of current formats, applications, limitations and perspectives for quantitative monitoring. We illustrate the potentials and limitations of analysis with lateral flow (immuno)assays using a literature survey and a SWOT analysis (acronym for "strengths, weaknesses, opportunities, threats"). Articles referred to in this survey were searched for on MEDLINE, Scopus and in references of reviewed papers. Search terms included "immunochromatography", "sol particle immunoassay", "lateral flow immunoassay" and "dipstick assay".

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A novel, rapid, single-step immunochromatographic procedure for the detection of mouse immunoglobulin

Cited by 40 publications

References 2 publications

Immunological Methods for Detection and Identification of Infectious Disease and Biological Warfare Agents

Immunological Methods for Detection and Identification of Infectious Disease and Biological Warfare Agents

Development and evaluation of a rapid lateral flow immunochromatographic strip assay for screening 19‐nortestosterone

Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

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