A novel, rapid and robust HPLC-ELSD method for simultaneous determination of fructose, glucose and sucrose in various food samples: Method development and validation

Soyseven, Murat; Sezgin, Burcu; Arlı, Göksel

doi:10.1016/j.jfca.2022.104400

Cited by 28 publications

(10 citation statements)

References 35 publications

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“…To minimize adverse effects, it is crucial to maintain low sugar consumption [ 10 , 11 ]. The quantification of sucrose is performed by using traditional analytical techniques like calorimetry [ 12 , 13 , 14 ], reverse-phase high-performance liquid chromatography (RP-HPLC) [ 15 ], high-performance liquid chromatography (HPLC) [ 16 , 17 , 18 , 19 ], florometry [ 20 , 21 ], calorimetry, polarimetry, and near-infrared spectroscopy [ 22 ]. These methods are time-consuming, expensive, and complex to implement, which are all major disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Efficient and Non-Enzymatic Electrochemical Sensors for the Detection of Sucrose

Asghar

Mustafa

Jabeen

et al. 2023

Sensors

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Molecularly imprinted polymers have been used for the creation of an electrochemical sensor for the detection of sucrose, which are modified by using functionalized graphene (fG). Using AIBN as the free radical initiator and sucrose as the template, imprinted polymers are synthesized. The monomer, 4,4′-diisocyanatodiphenylmethane (DPDI), has both proton donor groups (N-H or O-H) and lone-pair donor groups (C=O). By creating H-bonds with electron donor groups (C=O), the proton donor group in this polymer may interact with the sugar molecule serving as its template. The sensor signals have improved as a result of the interaction between the monomer and the template. Thermogravimetric and differential thermal analysis (TGA/DTA) curves, scanning electron microscopy (SEM), and FT-IR spectroscopy have been employed to characterize the fabricated receptors. The fabricated sensor has exhibited a limit of detection of 16 ppb for the target analyte that is highly sensitive, linear, reversible, regenerative, and selective. Moreover, the sensor’s stability, reproducibility, and reusability have been evaluated for six months, following the device’s manufacturing, and the results revealed similar responses with the percentage error of less than 1%. Most importantly, this sensor has demonstrated a quick response time, which is very sensitive, stable, and selective.

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Section: Introductionmentioning

confidence: 99%

Fabrication of Efficient and Non-Enzymatic Electrochemical Sensors for the Detection of Sucrose

Asghar

Mustafa

Jabeen

et al. 2023

Sensors

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“…As we comprehensively discussed in our recent publication [ 3 ], chromatographic and spectroscopic techniques, alongside conventional methods measuring physicochemical properties, such as electrical conductivity or acidity, are commonly applied in the field. Although spectroscopic methods usually provide non-destructive analysis and conventional methods are widely available due to their low-cost, combining chromatography with mass spectrometry (MS) permits the detection of various analyte classes, e.g., pesticide residues (to check bio-production) [ 4 ], sugars (to evaluate quality characteristics towards established regulation, Directive 2001/110/EC) [ 5 ] or phenolic compounds (to estimate geographical, botanical or entomological origin) [ 6 , 7 ]. Importantly, phenolic compounds determination in honey authenticity studies can have a binary character, in detail, both as characteristic markers as well as to evaluate the nutritional value of honey due to their bioactivity [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Thorough Investigation of the Phenolic Profile of Reputable Greek Honey Varieties: Varietal Discrimination and Floral Markers Identification Using Liquid Chromatography–High-Resolution Mass Spectrometry

et al. 2022

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Honey is a highly consumed commodity due to its potential health benefits upon certain consumption, resulting in a high market price. This fact indicates the need to protect honey from fraudulent acts by delivering comprehensive analytical methodologies. In this study, targeted, suspect and non-targeted metabolomic workflows were applied to identify botanical origin markers of Greek honey. Blossom honey samples (n = 62) and the unifloral fir (n = 10), oak (n = 24), pine (n = 39) and thyme (n = 34) honeys were analyzed using an ultra-high-performance liquid chromatography hybrid quadrupole time-of-flight mass spectrometry (UHPLC-q-TOF-MS) system. Several potential authenticity markers were revealed from the application of different metabolomic workflows. In detail, based on quantitative targeted analysis, three blossom honey markers were found, namely, galangin, pinocembrin and chrysin, while gallic acid concentration was found to be significantly higher in oak honey. Using suspect screening workflow, 12 additional bioactive compounds were identified and semi-quantified, achieving comprehensive metabolomic honey characterization. Lastly, by combining non-targeted screening with advanced chemometrics, it was possible to discriminate thyme from blossom honey and develop binary discriminatory models with high predictive power. In conclusion, a holistic approach to assessing the botanical origin of Greek honey is presented, highlighting the complementarity of the three applied metabolomic approaches.

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“…Unlike oil, sugar is polar and nonvolatile; hence, analysis is performed using high-performance liquid chromatography (HPLC). Analysis of three types of sugars has been carried out using the HPLC with evaporative light scattering detection (ELSD) method [35][36][37][38][39][40]. ELSD detector has succeeded in detecting 13 types of sugars in foods [41].…”

Section: Introductionmentioning

confidence: 99%

Nutritional Comparison of Sacha Inchi (Plukenetia volubilis) Residue with Edible Seeds and Nuts in Taiwan: A Chromatographic and Spectroscopic Study

Yanti

Agrawal

Saputri

et al. 2022

International Journal of Food Science

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Sacha inchi is a source of quality commercial oil in Taiwan. Oil extraction results in sacha inchi residue have not been utilized and not much investigated. Different edible seeds and nuts have different levels of nutrients. This study aims (a) to determine the oil, moisture, ash, protein, carbohydrate, type of fatty acid, resveratrol, and type of sugar in edible seeds and nuts, including sacha inchi residue, and (b) to determine the model to predict the five macronutrients using NIR spectroscopy. The samples used were candlenut, peanut, sesame, sunflower, sacha inchi residue, and black bean. Determination was conducted using NIR spectroscopy, NMR spectroscopy, LC-MS/MS, and HPLC-ELSD. NIR spectroscopy prediction results show that candlenut is rich in oil, and sacha inchi residue is rich in minerals, protein, and moisture. The correct prediction model for oil and moisture is principal component regression, while partial least squares are for ash, protein, and carbohydrates. NMR spectroscopy results showed that all samples were rich in polyunsaturated fatty acids. Sacha inchi residue is rich in omega 3. LC-MS/MS results showed that all samples contained resveratrol, and its highest level was found in sesame. HPLC-ELSD results showed eight types of sugars in the samples. High sucrose was found in sacha inchi residue, sunflower, sesame, and candlenut. The results are expected to provide information on nutrient levels in seeds and nuts to consumers and people who deal with nutrition. Also, results are expected to increase the economic value of sacha inchi residue as a source of diversification of food products in Taiwan.

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A novel, rapid and robust HPLC-ELSD method for simultaneous determination of fructose, glucose and sucrose in various food samples: Method development and validation

Cited by 28 publications

References 35 publications

Fabrication of Efficient and Non-Enzymatic Electrochemical Sensors for the Detection of Sucrose

Fabrication of Efficient and Non-Enzymatic Electrochemical Sensors for the Detection of Sucrose

Thorough Investigation of the Phenolic Profile of Reputable Greek Honey Varieties: Varietal Discrimination and Floral Markers Identification Using Liquid Chromatography–High-Resolution Mass Spectrometry

Nutritional Comparison of Sacha Inchi (Plukenetia volubilis) Residue with Edible Seeds and Nuts in Taiwan: A Chromatographic and Spectroscopic Study

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