A novel QTL governing resistance to stem rot disease caused by Sclerotium rolfsii in peanut

Bera, SK; Kamdar, J. H.; Kasundra, S. V.; Ajay, B. C.

doi:10.1007/s13313-016-0448-x

Cited by 26 publications

(15 citation statements)

References 37 publications

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“…The present study reports development of highquality genetic map with 585 SNP loci with spanning the distance of 2430 cM with an average inter-marker distance of 4.1 cM. Earlier, Bera et al (2017) tried to develop SSRbased genetic map for conducting mapping for the same (Bertioli et al 2016;Chen et al 2016) and now cultivated tetraploid genomes have enhanced the precision in SNP and candidate gene discovery in groundnut. Genetic dissection and QTL discovery analysis indicated complex genetic nature for stem rot resistance Use of two different genetic softwares showed difference in results where QTL cartographer showed relatively higher PVE than ICIM.…”

Section: Sequencing-based Genotyping Facilitated Development Of High-mentioning

confidence: 90%

“…Genetic dissection and QTL discovery analysis indicated complex genetic nature for stem rot resistance Use of two different genetic softwares showed difference in results where QTL cartographer showed relatively higher PVE than ICIM. A lone study conducted with just 12 mapped loci for stem rot resistance in groundnut reported one major QTL qstga01.1 with flanking markers GM2350 and TC4H02 (Bera et al 2017). The high-quality SNP-based genetic map developed in present study allowed to conduct high-resolution genetic mapping leading to detection of 7 main-effect QTLs for stem rot resistance at different infection periods (6 DAI, 9 DAI, 12 DAI) with cumulative 47.4% PVE.…”

Section: Sequencing-based Genotyping Facilitated Development Of High-mentioning

confidence: 99%

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Genotyping-by-sequencing based genetic mapping reveals large number of epistatic interactions for stem rot resistance in groundnut

et al. 2018

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Key message Genetic mapping identified large number of epistatic interactions indicating the complex genetic architecture for stem rot disease resistance. Abstract Groundnut (Arachis hypogaea) is an important global crop commodity and serves as a major source of cooking oil, diverse confectionery preparations and livestock feed. Stem rot disease caused by Sclerotium rolfsii is the most devastating disease of groundnut and can cause up to 100% yield loss. Genomic-assisted breeding (GAB) has potential for accelerated development of stem rot resistance varieties in short period with more precision. In this context, linkage analysis and quantitative trait locus (QTL) mapping for resistance to stem rot disease was performed in a bi-parental recombinant inbred line population developed from TG37A (susceptible) × NRCG-CS85 (resistant) comprising of 270 individuals. Genotyping-bysequencing approach was deployed to generate single nucleotide polymorphism (SNP) genotyping data leading to development of a genetic map with 585 SNP loci spanning map distance of 2430 cM. QTL analysis using multi-season phenotyping and genotyping data could not detect any major main-effect QTL but identified 44 major epistatic QTLs with phenotypic variation explained ranging from 14.32 to 67.95%. Large number interactions indicate the complexity of genetic architecture of resistance to stem rot disease. A QTL of physical map length 5.2 Mb identified on B04 comprising 170 different genes especially leucine reach repeats, zinc finger motifs and ethyleneresponsivefactors, etc., was identified. The identified genomic regions and candidate genes will further validate and facilitate marker development to deploy GAB for developing stem rot disease resistance groundnut varieties.Communicated by Henry T. Nguyen. Electronic supplementary materialThe online version of this article (https ://doi.org/10.1007/s0012 2-018-3255-7) contains supplementary material, which is available to authorized users. * Rajeev K. Varshney

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Section: Sequencing-based Genotyping Facilitated Development Of High-mentioning

confidence: 90%

Section: Sequencing-based Genotyping Facilitated Development Of High-mentioning

confidence: 99%

Genotyping-by-sequencing based genetic mapping reveals large number of epistatic interactions for stem rot resistance in groundnut

et al. 2018

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“…Collar rot resistance is under polygenic control, like, groundnut (Bera et al 2016, Cui et al 2019Dodia et al 2019) andalfalfa (Prat et al 2002), where the genotype factors controlling the trait are higher than the environmental factors observed. The resistance of LL56, as opposed to the susceptible genotype L4710, was con rmed by comparing the mycelial load in twelve-day-old seedlings after arti cial inoculation by quantitative real-time PCR.…”

Section: Discussionmentioning

confidence: 99%

“…The disease also hinders efforts to expand the land under lentil cultivation in rice fallow, especially in India (Tarafder et al, 2018). This disease is also prevalent in several lentil growing countries like China, Pakistan, Nepal, and Bangladesh and also infects other legumes such as chickpea and groundnut (Bera et al 2016;Dodia et al 2019). The disease manifests water-soaked symptoms on stems, and nally, rotting of seedling stem occurs.…”

Section: Introductionmentioning

confidence: 99%

Role of temperature, seedling-age and soil moisture on collar rot disease in lentil caused by Sclerotium rolfsii and identification of resistance sources

Roy

Das

Sagolsem

et al. 2021

Preprint

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Collar rot disease caused by Sclerotium rolfsii attacks lentils at the seedling stage and reduces plant population considerably in the field. Although soil moisture and temperature influence disease development much, no concrete attempts to find the optimum level of moisture, temperature, and seedling age have been made in lentils. Here, we identified optimum soil moisture, temperature, and seedling age that allow successful infection by S. rolfsii and maximum seedling mortality in a controlled environment inside a plant growth chamber. Screening of one hundred and ninety genotypes for two consecutive years in the field identified some resistant genotypes. Ten genotypes were selected from earlier screening and further evaluated in sick plots at two different locations with different pH for three consecutive years. About 80% variation in disease resistance was due to genotypes, and minor infection of collar rot disease was observed in acidic lateritic soil than in neutral alluvial soil. Furthermore, low mycelial load in a selected resistant genotype was confirmed by quantitative real-time PCR using an S. rolfsii specific primer pair. The identified resistant genotypes will be helpful to breeding collar rot resistant cultivars and mapping disease resistance. In addition, the study improves the understanding of the development of collar rot disease, which is critical for expanding the area under cultivation in Asia, especially in rice fallows.

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“…Moreover, we also used mutation breeding to induce fresh alleles/traits for different biotic and abiotic stresses in peanut over 35 year at ICAR-DGR, Junagadh and developed several desirable PBLs of SB, VB and VR growth habit. These inter-specific/intra-specific/mutant PBLs are being regularly used as donors in our different ongoing peanut improvement program (Bera et al 2008(Bera et al , 2010(Bera et al , 2011(Bera et al , 2012(Bera et al , 2014(Bera et al , 2016. However, in the course of developing these 563 PBLs our breeding objectives never directed to ahFAD2 alleles since it was reported later (Chen et al 2010).…”

Section: Plant Materialsmentioning

confidence: 99%

Fatty acid desaturase-2 (ahFAD2) mutant alleles in peanut (Arachis hypogaea L.) pre-breeding lines: an insight into the source, features, discourse, and selection of novel pre-breeding lines

Kamdar

Jasani

Ajay

et al. 2020

Genet Resour Crop Evol

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High oleic peanuts and derived food products offer longer shelf life benefits to the food processing industry in addition to multiple health benefits to the consumers. The two mutant alleles, ahFAD2A and ahFAD2B control composition of oleic, linoleic and palmitic acid content in peanut. A total of 563 peanut pre-breeding lines were tested for the presence ahFAD2A and ahFAD2B mutant alleles using allele specific markers. The ahFAD2A mutant allele was present in 82 lines, while none of these lines had ahFAD2B mutant allele. Among botanical types, ahFAD2A mutant allele was more frequent in lines with Virginia growth habit than Spanish bunch although no correlation of ahFAD2A mutant allele with high oleic acid content and growth habit could be established. Oleic and linoleic acid content in 82 prebreeding lines ranged from 39.70 to 62.70% and 17.76 to 31.95%, respectively, with maximum oleic to linoleic acid ratio of 4. Oleic acid was found to be negatively correlated with linoleic and palmitic acid. Further, pre-breeding lines with ahFAD2A mutant allele, high oleic content and high oleic to linoleic ratio were investigated and novel lines were identified for resistance to late leaf spot, short duration, higher pod yield and other yield related traits. These novel pre-breeding lines can be used as a potential donor in peanut improvement programme and to diversify the primary gene pool including initiating further research on induction of fresh ahFAD2B mutant allele.

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A novel QTL governing resistance to stem rot disease caused by Sclerotium rolfsii in peanut

Cited by 26 publications

References 37 publications

Genotyping-by-sequencing based genetic mapping reveals large number of epistatic interactions for stem rot resistance in groundnut

Genotyping-by-sequencing based genetic mapping reveals large number of epistatic interactions for stem rot resistance in groundnut

Role of temperature, seedling-age and soil moisture on collar rot disease in lentil caused by Sclerotium rolfsii and identification of resistance sources

Fatty acid desaturase-2 (ahFAD2) mutant alleles in peanut (Arachis hypogaea L.) pre-breeding lines: an insight into the source, features, discourse, and selection of novel pre-breeding lines

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