A Novel Pseudomonas aeruginosa Strain with an
            <i>oprD</i>
            Mutation in Relation to a Nosocomial Respiratory Infection Outbreak in an Intensive Care Unit

Yan, Yingjun; Yao, Xiangyu; Li, Haijing; Zhou, Zhen; Wen-fang, Huang; Stratton, Charles W.; Lu, Chung‐Dar; Tang, Yi‐Wei

doi:10.1128/jcm.02782-14

Cited by 8 publications

(8 citation statements)

References 13 publications

(10 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mutational inactivation of OprD is primarily responsible for the resistance to imipenem, and is reported to be the most common mechanism in China and Korea ( Fang et al, 2014 ; Kim et al, 2016 ). Frameshifts, caused by deletions or insertions of 1 or more base pairs, are the most frequent type of mutation ( Rodriguez-Martinez et al, 2009 ; Yan et al, 2014 ). The insertion of two different IS elements was also common (18 isolates).…”

Section: Discussionmentioning

confidence: 99%

Molecular Typing and Carbapenem Resistance Mechanisms of Pseudomonas aeruginosa Isolated From a Chinese Burn Center From 2011 to 2016

et al. 2018

View full text Add to dashboard Cite

Pseudomonas aeruginosa is the leading cause of infection in burn patients. The increasing carbapenem resistance of P. aeruginosa has become a serious challenge to clinicians. The present study investigated the molecular typing and carbapenem resistance mechanisms of 196 P. aeruginosa isolates from the bloodstream and wound surface of patients in our burn center over a period of 6 years. By multilocus sequence typing (MLST), a total of 58 sequence types (STs) were identified. An outbreak of ST111, a type that poses a high international risk, occurred in 2014. The isolates from wound samples of patients without bacteremia were more diverse and more susceptible to antibiotics than strains collected from the bloodstream or the wound surface of patients with bacteremia. Importantly, a large proportion of the patients with multisite infection (46.51%) were simultaneously infected by different STs in the bloodstream and wound surface. Antimicrobial susceptibility testing of these isolates revealed high levels of resistance to carbapenems, with 35.71% susceptibility to imipenem and 32.14% to meropenem. To evaluate mechanisms associated with carbapenem resistance, experiments were conducted to determine the prevalence of carbapenemase genes, detect alterations of the oprD porin gene, and measure expression of the ampC β-lactamase gene and the mexB multidrug efflux gene. The main mechanism associated with carbapenem resistance was mutational inactivation of oprD (88.65%), accompanied by overexpression of ampC (68.09%). In some cases, oprD was inactivated by insertion sequence element IS1411, which has not been found previously in P. aeruginosa. These findings may help control nosocomial P. aeruginosa infections and improve clinical practice.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular Typing and Carbapenem Resistance Mechanisms of Pseudomonas aeruginosa Isolated From a Chinese Burn Center From 2011 to 2016

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Pseudomonas aeruginosa, a notorious primary opportunistic pathogen, is the leading cause of hospital-acquired infections worldwide and commonly associated with high fatality and morbidity rates 1 . Among the most common Gram negative bacteria, P. aeruginosa is the most frequently isolated worrisome pathogen that causes life threatening respiratory tract infection, urinary tract infections in patients admitted to intensive care units 2,3 . Infections caused by this pathogen are often difficult to be treated because of both its intrinsic resistance and its remarkable ability to acquire further resistance mechanisms to a plethora of antibiotics 4,5 .…”

Section: Introductionmentioning

confidence: 99%

Detection of blaSPM-1 and blaSIM-2 Metallo-β-Lactamases Genes in Imipenem-Resistant Pseudomonas aeruginosa Clinical Isolates Recovered from Two University Hospitals in Egypt

Emara

El-Domany

El-Magd

et al. 2020

Journal of Advanced Pharmacy Research

View full text Add to dashboard Cite

Objectives: Unraveling mechanisms whereby Pseudomonas aeruginosa becomes resistant to carbapenems through testing 114 non-duplicate P. aeruginosa clinical isolates for their susceptibility to various classes of antibiotics and scrutinizing the production of metallo-β-lactamases (MBLs) by tested isolates. Methods: Susceptibility testing of P. aeruginosa to different antibiotics was determined by Kirby-Bauer disk diffusion method and MBLs production by tested isolates was studied phenotypically and genotypically and PCR products were confirmed by sequencing Results: All tested clinical isolates showed eminent resistance to the majority of tested antibiotics and 14 isolates were imipenem (IPM)-resistant. Furthermore, IPM-resistant isolates were verified to be MBLs-producers. MBLs-encoding genes blaSIM and blaSPM genes were detected by PCR where four isolates were found to harbor blaSPM gene while only one isolate harbored blaSIM gene. The correct size of PCR products of blaSIM and blaSPM genes were sequenced and sequences were submitted to the GenBank databases and assigned the accession numbers KX452682 and KX452683 for blaSIM-2 and blaSPM-1, respectively. Conclusion: Here, we report the emanation of P. aeruginosa clinical isolates harboring blaSPM-1 and blaSIM-2 genes. This may reflect the substantial increase in the rate of imipenem resistance due to MBL in P. aeruginosa clinical isolates from Egypt. Early detection and infection-control practices are of the best antimicrobial strategy for combating this organism.

show abstract

“…Have reported an insertion sequence element (ISRP10) that causes to disrupt the oprD gene and seen in 96% of imipenem-resistant Pseudomonas aeruginosa isolates. In a study performed by Yingjun Yan et al (28). The result of the analysis, indicating that the 4-bp insertion in the oprD gene resulted in a frameshift in the OprD gene and imipenem resistance.…”

Section: Discussionmentioning

confidence: 99%

Upstream Region of OprD Mutations in Imipenem-Resistant and Imipenem-Sensitive Pseudomonas Aeruginosa Isolates

Kiani

Astani

Afkhami

et al. 2020

Preprint

View full text Add to dashboard Cite

Abstract Background: The current study was aimed to investigate the prevalence of the mutations of the oprD gene among imipenem-resistant and -sensitive Pseudomonas aeruginosa isolated from educational hospitals in Yazd, Iran.Methods: In this study, 90 P. aeruginosa isolates were collected from different clinical samples and transferred to the Department of Microbiology, Shahid Sadoghi University of Medical Science, during 2015 to 2016. All isolates were identified by the conventional biochemical tests and antibiotic resistance was determined using disk diffusion method. E. test was performed to determine the minimum inhibitory concentrations (MIC) of imipenem. The mutations of upstream of the oprD coding region and its promoters and 54 primary nucleotide of this gene were recognized by the amplification of this region and subsequently sequenced.Results: Seventy (77.7%) of isolates had MIC≥16 and were resistant to imipenem. The results showed that the rate of resistance to imipenem is increasing. Mutations of the upstream of the oprD gene and its promoters were seen in 25 (86.2%) of isolates and 4 strains had no mutation. All of the imipenem-resistant isolates had mutations. One isolate had a base substitution A→ C at nt 25 in coding region and this isolate had a point mutation leading to an amino acid change at positions 9 (I→L). Conclusion: The results showed that imipenem resistance is increasing in P. aeruginosa, also indicated that the point mutations were the most common cause of the inactivation of upstream of the oprD coding region among P. aeruginosa isolates, it seems this mechanism is effective in resistance of isolates to imipenem.

show abstract

A Novel Pseudomonas aeruginosa Strain with an oprD Mutation in Relation to a Nosocomial Respiratory Infection Outbreak in an Intensive Care Unit

Cited by 8 publications

References 13 publications

Molecular Typing and Carbapenem Resistance Mechanisms of Pseudomonas aeruginosa Isolated From a Chinese Burn Center From 2011 to 2016

Molecular Typing and Carbapenem Resistance Mechanisms of Pseudomonas aeruginosa Isolated From a Chinese Burn Center From 2011 to 2016

Detection of blaSPM-1 and blaSIM-2 Metallo-β-Lactamases Genes in Imipenem-Resistant Pseudomonas aeruginosa Clinical Isolates Recovered from Two University Hospitals in Egypt

Upstream Region of OprD Mutations in Imipenem-Resistant and Imipenem-Sensitive Pseudomonas Aeruginosa Isolates

Contact Info

Product

Resources

About