A novel protein phosphorylation pathway involved in osmotic-stress response in tobacco plants

Kodama, Yutaka; Tamura, Takashi; Hirasawa, W.; Nakamura, Kimiyo; Sano, Hiroshi

doi:10.1016/j.biochi.2009.01.003

Cited by 8 publications

(11 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1c). A phylogenetic dendrogram indicated that AFSK was most closely related to NtNAK (GenBank accession number ) (Kodama et al. 2009).…”

Section: Resultsmentioning

confidence: 99%

“…1c). A phylogenetic dendrogram indicated that AFSK was most closely related to NtNAK (GenBank accession number DQ447159) (Kodama et al 2009). The analysis indicated that AFSK clusters with NtNAK and PBS1 in a branch distinct from MLPK, ACIK1 and BIK1.…”

Section: Resultsmentioning

confidence: 99%

“…M‐locus protein kinase (MLPK) functions in self‐incompatibility signalling (Kakita et al. 2007), and Nicotiana tabacum NAK ( NtNAK ) plays a role in osmotic‐stress responses (Kodama et al. 2009).…”

mentioning

confidence: 99%

“…For example, BIK1 (Botrytis-induced kinase 1) (Lu et al 2010), PBS1 (avrPphB susceptible 1) ) and ACIK1 (Avr9/Cf-9 induced kinase 1) (Rowland et al 2005) are involved in disease resistance. M-locus protein kinase (MLPK) functions in selfincompatibility signalling (Kakita et al 2007), and Nicotiana tabacum NAK (NtNAK) plays a role in osmotic-stress responses (Kodama et al 2009). However, little is known about the functions of NAK-type protein kinases.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of fertilization and flower abscission following over‐expression of an apple NAK‐type protein kinase in tomato

Kim¹,

Yoon

Lee

2011

Plant Breeding

View full text Add to dashboard Cite

The Ser/Thr protein kinase subfamily of the novel Arabidopsis protein kinase (NAK) family is unique to plants and is not found in animals or prokaryotes, suggesting that NAKs play specific signalling roles in the control of plant growth and development. However, little is known about the function of NAK-type protein kinases. Here, we used a transgenic tomato plant system to examine the biological functions of AFSK, a NAK-type protein kinase isolated from apple fruits. Gain-offunction transgenic tomato plants containing the AFSK gene were developed by Agrobacterium-mediated transformation and plant regeneration. The plants had normal flower phenotype with respect to their overall shapes and sizes. However, the plants displayed decreased self-fertilization and early flower drop at the floral abscission zone following petal loss. These results suggest that AFSK affects pollen development and promotes floral abscission.

show abstract

“…1c). A phylogenetic dendrogram indicated that AFSK was most closely related to NtNAK (GenBank accession number ) (Kodama et al. 2009).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of fertilization and flower abscission following over‐expression of an apple NAK‐type protein kinase in tomato

Kim¹,

Yoon

Lee

2011

Plant Breeding

View full text Add to dashboard Cite

show abstract

“…Recently it was shown that NtNAK, a RLCK from class VII is an osmo-stress responsive protein kinase and phosphorylates a NtCK25 protein which is similar to human nuclear body protein SP110 involved in DNA/protein binding regulation (Kodama et al 2009). …”

Section: In Abscission Andmentioning

confidence: 99%

Characterization of ROP GTPase-activated Arabidopsis receptor-like cytoplasmic kinases (RLCK class VI_A)

Manuela

View full text Add to dashboard Cite

A Glycine soja ABA-responsive receptor-like cytoplasmic kinase, GsRLCK, positively controls plant tolerance to salt and drought stresses

Sun

Luo

et al. 2013

Planta

View full text Add to dashboard Cite

Receptor such as protein kinases are proposed to work as sensors to initiate signaling cascades in higher plants. However, little is known about the precise functions of receptor such as protein kinases in abiotic stress response in plants, especially in wild soybean. Here, we focused on characterization of the biological functions of a receptor-like cytoplasmic serine/threonine protein kinase gene, GsRLCK, which was previously identified as a putative salt-alkali stress-related gene from the transcriptome profiles of Glycine soja. Bioinformatic analysis showed that GsRLCK protein contained a conserved kinase catalytic domain and two transmembrane domains at the N-terminus, but no typical extracellular domain. Consistently, GsRLCK-eGFP fusion protein was observed on the plasma membrane, but eGFP alone was distributing throughout the cytoplasm in onion epidermal cells. Quantitative real-time PCR analysis revealed the induced expression of GsRLCK by ABA, salt, alkali, and drought stresses. However, the expression levels of GsRLCK seemed to be similar in different tissues, except soybean pod. Phenotypic assays demonstrated that GsRLCK overexpression decreased ABA sensitivity and altered expression levels of ABA-responsive genes. Furthermore, we also found that GsRLCK conferred increased tolerance to salt and drought stresses and increased expression levels of a handful of stress-responsive genes, when overexpressing in Arabidopsis. In a word, we gave exact evidence that GsRLCK was a novel receptor-like cytoplasmic protein kinase and played a crucial role in plant responses to ABA, salt, and drought stresses.

show abstract

A novel protein phosphorylation pathway involved in osmotic-stress response in tobacco plants

Cited by 8 publications

References 23 publications

Evaluation of fertilization and flower abscission following over‐expression of an apple NAK‐type protein kinase in tomato

Evaluation of fertilization and flower abscission following over‐expression of an apple NAK‐type protein kinase in tomato

Characterization of ROP GTPase-activated Arabidopsis receptor-like cytoplasmic kinases (RLCK class VI_A)

A Glycine soja ABA-responsive receptor-like cytoplasmic kinase, GsRLCK, positively controls plant tolerance to salt and drought stresses

Contact Info

Product

Resources

About