Characterization of ROP GTPase-activated Arabidopsis receptor-like cytoplasmic kinases (RLCK class VI_A)

The Effects of Exogenous Polyamines and the Silencing of RLCK VI_A Kinases on the Polar Growth of Nicotiana tabacum (Tobacco) Pollen Tubes

“…Plants have a unique family of small GTPases, which termed as Rho-of-plants (ROPs). ROP GTPases are members of the RAS superfamily of small GTP-binding proteins, which function as two-state molecular switches depending on their confirmation such as GDP-bound (inactive form) or GTP-bound (active form) (Jurca 2011;Lundquist 2006;Wennerberg, Rossman, and Der 2005). The ROP GTPases have many effector and regulator partners including potential ROP effector kinases, which belong to the large family of plant receptor-like kinases (RLK) (Dorjgotov et al 2009;Jurca et al 2008).…”

“…Plants have a unique family of Rho-type small GTPases, which termed as Rho-of-plants (ROPs). ROP GTPases are member of the RAS superfamily of small GTP-binding proteins, which function as two-state molecular switches depending on their confirmation such as GDPbound (inactive form) or GTP-bound (active form) (Jurca 2011;Lundquist 2006;Wennerberg et al 2005). The cycling of Rho proteins between active and inactive states is dependent on other regulatory proteins.…”

“…In Arabidopsis, around a thousand of RLK genes have been identified (Jurca 2011;Walker and Zhang 1990). Based on the presence/absence of the extracellular domains, the RLK family subdivided into 46 different subfamilies.…”

“…The RLCKs based on structure are subdivided into 12 families (RLCK I to XII) which has193 protein-coding genes (Haffani, Silva, and Goring 2004;Jurca 2011;Bleecker 2001, 2003). However, the alignment result showed that the At RLCK VI kinase family has 14 members belonging to two groups in Arabidopsis (Jurca et al 2008) that the first group is RLCK VI_A (ROP-interacting) and the second group is RLCK VI_B ( ROP -non-interacting).…”