A novel population health approach: Using fish retinoblastoma gene profiles as a surrogate for humans

Rotchell, Jeanette M.; Corbier, Frances A. du; Stentiford, Grant D.; Lyons, Brett P.; Liddle, Andrew R.; Ostrander, Gary K.

doi:10.1016/j.cbpc.2008.09.009

Cited by 5 publications

(10 citation statements)

References 33 publications

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“…The differences between benign (HA) and malignant (HC) liver tumor expression profiles are likely due to differences in their growth and invasive properties. Recent studies assessing retinoblastoma allele status in tumors (HC and HA) and normal liver tissues of dab sampled from similar geographical locations as the present study have also indicated that it is possible to use genetic information to predict sample phenotype (Rotchell et al 2009). Due to the relatively low replicate numbers (n = 5, 6) and high inter-individual variability, the specific groups of 'predictive genes' will require further testing with an increased number of additional samples to fully validate these transcriptional signatures.…”

Section: Discussionsupporting

confidence: 58%

Gene expression analyses of hepatocellular adenoma and hepatocellular carcinoma from the marine flatfish Limanda limanda

Small¹,

Williams²,

Sturve³

et al. 2010

Dis. Aquat. Org.

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At selected sites around the UK, the offshore sentinel flatfish species dab Limanda limanda are found to contain elevated levels of macroscopic liver tumors. Previous proteomic and metabolomic studies have demonstrated that differences exist between tumor and non-tumor tissues; however, these differing features were not identified, and little is known about the changes at the gene expression level, or whether prognostic markers are present and can be identified. A flounder Platichthys flesus custom cDNA microarray and RT-PCR were used to investigate hepatic mRNA expression in the histologically confirmed tumors, hepatocellular adenoma (HA) and hepatocellular carcinoma (HC) from dab, and in adjacent normal tissue from the same fish. Differences in gene expression were observed between tumor and normal tissues, and between tumor types. A class-prediction approach using 50 transcripts revealed sufficient group-specific expression profiles to allow segregation of samples dependent on their tumor type or the sex of the host. Vitellogenins were found to display the greatest induction (up to 500-fold induction) in some HC tumors from female fish and in both HA and HC tumors from males. To the best of our knowledge, this is the first report of the association of vitellogenin expression with tumors of wild fish.

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Section: Discussionsupporting

confidence: 58%

Gene expression analyses of hepatocellular adenoma and hepatocellular carcinoma from the marine flatfish Limanda limanda

Small¹,

Williams²,

Sturve³

et al. 2010

Dis. Aquat. Org.

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“…Rotchell et al, (2009- this issue) analyzed tumors in livers of marine flatfishes for mutations in theretinoblastoma ( Rb ) gene, a tumor suppressor. Rb gene alterations were localized to ademomas and carcinomas but not to normal adjacent tissue.…”

Section: ) Models Of Carcinogenesis/mutagenesismentioning

confidence: 99%

Aquatic animal models of human disease: Selected papers and recommendations from the 4th Conference

Hinton

Hardman

Kullman

et al. 2009

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…Dab possess both a similar histopathological tumour profile to humans (Stern and Zon, 2003) and homologs of human cancer genes such as ras and retinoblastoma (Rb), including mutational alterations of the Rb gene in tumour tissues (Du Corbier et al, 2005). In this respect, we have previously proposed that the dab hepatocellular tumour development could act as surrogate for cancer and the tumourigenesis process in human populations (Rotchell et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…This diagnostic approach has recently been coupled with molecular analyses of tumour and surrounding normal tissue (Small et al, 2010). In addition, gross lesions and apparently normal tissues have been resected from the dab liver for molecular investigations such as genetic alterations of neoplastic associated genes (Du Corbier et al, 2005;Rotchell et al, 2009, Lerebours et al, 2014, transcriptomic (Small et al, 2010), proteomic (Stentiford et al, 2005) and metabolomic studies (Stentiford et al, 2005;Southam et al, 2008). Traditional microdissection and molecular analysis of populations of cells presents a challenge in that cellular heterogeneity within tissues samples may result in misleading findings (Cole et al, 1999;Sluka et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Hepatocellular adenoma in a European flatfish (Limanda limanda): Genetic alterations in laser-capture micro-dissected tissue and global transcriptomic approach

Lerebours

Chapman

Lyons

et al. 2017

Marine Pollution Bulletin

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Liver tumours in flatfish have been diagnosed using histopathology for decades to monitor the impacts of marine pollution. Here we describe the application of specific gene (retinoblastoma, Rb) profiling in laser capture micro-dissected samples, and a suppression subtractive hybridization (SSH) approach to isolate differentially expressed genes in hepatocellular adenoma (HCA) samples from dab, Limanda limanda. The Rb profiles from apparently normal and HCA micro-dissected samples of fish from the North Sea showed no significant difference, and genotypic heterogeneity within defined histological phenotypes was observed. In the SSH, sequences associated with cell signalling, cell cycle, gene expression regulation, protein transport and protein degradation were isolated. These included up-regulation of arrestin domain containing 3 (arrdc3), Rac-1 and tribbles, and down-regulation of ankyrin repeat/sterile alpha-motif domain-containing protein 1B-like (ANKS1B-like), c-fos, CDKN1B and RhoA-like sequences, previously implicated in mammalian HCA. This study offers new candidates involved in fish liver tumour development.

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A novel population health approach: Using fish retinoblastoma gene profiles as a surrogate for humans

Cited by 5 publications

References 33 publications

Gene expression analyses of hepatocellular adenoma and hepatocellular carcinoma from the marine flatfish Limanda limanda

Gene expression analyses of hepatocellular adenoma and hepatocellular carcinoma from the marine flatfish Limanda limanda

Aquatic animal models of human disease: Selected papers and recommendations from the 4th Conference

Hepatocellular adenoma in a European flatfish (Limanda limanda): Genetic alterations in laser-capture micro-dissected tissue and global transcriptomic approach

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