A novel polymorphism in the bovine insulin‐like growth factor binding protein‐3 (IGFBP3) gene

Polymorphisms of the insulin-like growth factor-binding protein 3 gene (IGFBP3) in gayal (Bos frontalis)

“…There have been previous reports of similar PCR products, derived using a same set of primers to that used in the present study, in cattle (Choudhary et al, 2007;Maciulla et al, 1997). In the current study, a sequence of 645 bp with nine SNPs (C14T, A122C, C137T, G144C, C155T, G213A, C279A, G334A and G460A) in intron 2 of gayal IGFBP3 was retrieved after direct sequencing of the 46 amplicons.…”

“…In a previous study, a polymorphic site in bovine IGFBP3 was reported firstly by Maciulla et al (1997) after using HaeIII endonuclease to digest DNA samples (HaeIII-RFLP method) from Holstein, Angus and Hereford cattle. This site subsequently was confirmed and identified as the SNP C279A in intron 2 by the sequencing method (Choudhary et al, 2007).…”

“…The full length of the IGFBP3 is 8.9 kb and it contains five encoding exons (Kim et al, 2005). Nucleotide sequences of the IGFBP3 have been determined in cattle (Haegeman et al, 1999;Maciulla et al, 1997;Shukla, 2001;Sun et al, 2002), buffaloes (Kumar et al, 2004;Padma et al, 2004), sheep (Kumar et al, 2002) and goats (Lan et al, 2007a(Lan et al, , 2007b, and association of the IGFBP3 genotypes with production traits has been reported for cattle (Choudhary et al, 2007;Sun et al, 2002). Due to the remoteness of their habitats and to socio-political and ecological factors, the gayal is one of the least studied ungulates (Mohan et al, 2007) and no such information is available for this interesting species.…”

“…The sequences of IGFBP3 were amplified using the primers IGFBP3-F (5′-CCAAGCGTGAGACAGAATAC-3′) and IGFBP3-R (5′-AGGAGGGA-TAGGAGCAAGAT-3′) (Maciulla et al, 1997). The PCR was carried out in a reaction volume of 25 μL, containing 2.0 μL DNA (approximately 30 ng μL − 1 ), 0.5 μL 20 mmol/L dNTPs, 2.5 μL buffer, 1.5 μL 25 mmol MgCl 2 , 1.0 μL 10 μmol/L forward primer, 1.0 μL 10 μmol/L reverse primer, 0.5 μL 10× Taq DNA polymerase (5 U/μL, Beijing TransGen Biotechnology Co., Ltd., China) and 16.0 μL distilled water.…”

The novel SNPs of the <i>IGFBP3</i> gene and their associations with litter size and weight traits in goat (Brief report)

“…Single nucleotide polymorphisms (SNPs) have been described in the bovine IGFBP3 gene which was associated with production traits, as well as in the buffalo (MACIULLA et al, 1997;PADMA et al, 2004). No polymorphism was detected in the sheep IGFBP3 gene (KUMAR et al, 2006).…”