A novel platform technology for the detection of genetic variations by surface plasmon resonance

Mertig, Michael; Kick, Alfred; Bönsch, Martin; Katzschner, Beate; Voigt, J.; Sonntag, Frank; Schilling, Niels; Klotzbach, Udo; Danz, Norbert; Begemann, Sebastian; Herr, Alexander; Jung, Martin

doi:10.1109/icsens.2009.5398247

Cited by 11 publications

(14 citation statements)

References 6 publications

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“…As described elsewhere [12,15] the primers with hexaethylene glycol (HEG) spacer and tag sequences (Table 1) have the structure 5 0 -tag-HEG-AGCCT-GAATGGCGAATGG-3 0 and the sequence of the reverse primer for synthesis of 300-bp-long PCR products is GACATTAACC-TATAAAACTAGGCGTATCA. As described elsewhere [12,15] the primers with hexaethylene glycol (HEG) spacer and tag sequences (Table 1) have the structure 5 0 -tag-HEG-AGCCT-GAATGGCGAATGG-3 0 and the sequence of the reverse primer for synthesis of 300-bp-long PCR products is GACATTAACC-TATAAAACTAGGCGTATCA.…”

Section: Chemicals and Pcr Productsmentioning

confidence: 99%

“…We analyze the products of a polymerase chain reaction (PCR) that had been tagged with specific single-stranded DNA (ssDNA) sequences [12]. Here, the analysis of genetic variations is demonstrated as an exemplary application.…”

Section: Introductionmentioning

confidence: 99%

“…Here, the analysis of genetic variations is demonstrated as an exemplary application. We analyze the products of a polymerase chain reaction (PCR) that had been tagged with specific single-stranded DNA (ssDNA) sequences [12]. The specific hybridization of these molecules onto the corresponding complementary ant-tag sequences by the SPR spectrometer is demonstrated.…”

Section: Introductionmentioning

confidence: 99%

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Surface plasmon resonance platform technology for multi‐parameter analyses on polymer chips

Danz

Kick

Sonntag

et al. 2011

Engineering in Life Sciences

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The development of a surface plasmon resonance (SPR) spectrometer comprising angular-resolved analysis for quasi-monochromatic illumination is reported. The optical system utilizes disposable, injection-molded chips combined with a lateral imaging optical system for parallel analysis of one-dimensional spot arrays. Further parallelization is achieved by introducing a segmented light source. This source sequentially illuminates three neighbored one-dimensional arrays in order to keep angular-resolved analysis without introducing any mechanically moving parts. This system is applied to detect genetic variations among different DNA samples obtained from polymerase chain reaction (PCR). For this purpose, 135 spots on the chip surface have been prepared by spotting and analyzed separately

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Section: Chemicals and Pcr Productsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Surface plasmon resonance platform technology for multi‐parameter analyses on polymer chips

Danz

Kick

Sonntag

et al. 2011

Engineering in Life Sciences

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“…One such SPR-biosensor, together with the relevant microfluidic equipment and lab-on-a-chip system, was developed by the Fraunhofer Institute for Applied Optics and Precision Engineering IOF (Jena, Germany) and the Fraunhofer Institute for Material and Beam Technology IWS (Dresden, Germany); the performance of this sensor has been demonstrated in various contexts [2][3][4][5][6][7]. This machine represents a low-cost biosensor, which is robust and easy to transport.…”

Section: Introductionmentioning

confidence: 99%

A compact and rapid aptasensor platform based on surface plasmon resonance

Henseleit¹,

Schmieder²,

Bley³

et al. 2011

Engineering in Life Sciences

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Aptamers are synthetic single-stranded oligonucleotides which bind specifically to their target. They offer several advantages over antibodies. For example, aptamers can be produced under unphysiological conditions against almost any target, including toxic or pathological substances. They are also quicker and cheaper produced than antibodies, and are easy to modify without loss of activity. Furthermore, they exhibit high stability under a width range of conditions. Consequently, they make excellent receptors for the use in biosensors. This article describes the evaluation of a novel aptasensor based on the Surface Plasmon Resonance (SPR)-system developed by the Fraunhofer Institute for Applied Optics and Precision Engineering IOF (Jena, Germany) using a thrombin–aptamer interaction as a model system. The biotin-tagged aptamer was attached to the sensor's gold surface by means of its interaction with streptavidin. Thrombin solutions of different concentrations were pumped over this surface, and the interaction was measured under buffer flow. The binding signals for the thrombin–aptamer interaction were compared to those arising from a control random-oligonucleotide of the same size and bearing the same modifications. Using this approach, we were able to obtain reproducible, significant and stable signals with a limit of detection of about 26 nmol/L

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“…The sensor surface of the system, a 50 nm × 12 mm × 3 mm gold layer, is illuminated by three selectable LEDs, each covering an area of (9 × 0.8) mm 2 (Henseleit et al, 2011;Mertig et al, 2009). A CCD camera with a spatial resolution of 1280 rows (spots) and an angledependent intensity distribution of 960 columns records the reflected light (Mertig et al, 2009).…”

Section: Spr Experimentsmentioning

confidence: 99%

Monitoring human serum albumin cell cultures using surface plasmon resonance (SPR) spectroscopy

Henseleit

Pohl

Bley

et al. 2015

J. Sens. Sens. Syst.

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Abstract. Continuously monitoring cell cultures is essential for both controlling critical parameters and improving understanding of key processes. An ideal technique in this context is surface plasmon resonance (SPR) spectroscopy, which essentially exploits changes in the angle of incident light that occur when molecules bind to a surface. It provides the ability to monitor real-time changes in small concentrations of various molecules, with no need for additional labels or sample preparation. Here we present an SPR-based immunoassay for monitoring concentrations of human serum albumin (HSA), and compare its sensitivity when used in conjunction with a Biacore platform and the cheaper, smaller li SPR system. In conjunction with either system, the immunoassay can detect HSA (a hepatocyte viability marker) at concentrations typically present in three-dimensional hepatocyte cultures mimicking the liver used to evaluate effects of drug candidates before exposure to humans or animals. Furthermore, in conjunction with the li SPR system, it is sufficiently sensitive to measure the much lower HSA levels present in skin-hepatocyte co-cultures.

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A novel platform technology for the detection of genetic variations by surface plasmon resonance

Cited by 11 publications

References 6 publications

Surface plasmon resonance platform technology for multi‐parameter analyses on polymer chips

Surface plasmon resonance platform technology for multi‐parameter analyses on polymer chips

A compact and rapid aptasensor platform based on surface plasmon resonance

Monitoring human serum albumin cell cultures using surface plasmon resonance (SPR) spectroscopy

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