A novel plant defensin-like gene of winter wheat is specifically induced during cold acclimation

Koike, Michiya; Okamoto, Takashi; Tsuda, Sakae; Imai, Ryozo

doi:10.1016/s0006-291x(02)02391-4

Cited by 115 publications

(92 citation statements)

References 38 publications

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“…In wheat, CHI3 was highly induced in the anther culture phase but its expression was also inconsistent between cultivars, being almost constitutive in the low-responding one. Although Tad1 induction was reported earlier in seedlings treated with low temperature (Koike et al 2002), in this experiment no expression was observed in cold-treated triticale microspores. Instead, Tad1 was expressed in in vitro cultured microspores, on 4dc or 8dc, but only in lines DH25 and DH28, respectively.…”

Section: Resultscontrasting

confidence: 70%

Changes in gene expression patterns associated with microspore embryogenesis in hexaploid triticale (×Triticosecale Wittm.)

Żur

Dubas

Krzewska

et al. 2013

Plant Cell Tiss Organ Cult

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To gain a better understanding of the molecular mechanisms controlling microspore embryogenesis (ME) in triticale (9Triticosecale Wittm.), the expression patterns of 13 genes, previously identified in bread wheat to be associated with microspore-derived embryo development, were analysed. Four triticale doubled haploid (DH) lines, significantly different with respect to embryogenic potential, were studied. The gene expression profile was dissected at different points of the ME induction procedure up to the 8th day of in vitro culture (dc). RT-PCR revealed that these 13 genes were expressed during triticale ME. Variations in gene expression profiles were observed between the studied DH lines. DH28 (highly embryogenic) was the only one in which all analysed genes (Ta.TPD1-like, TAA1b, GSTF2, GSTA2, CHI3, Tad1, XIP-R1, Ta-AGL14, TaNF-YA7, SERK2, SERK1, TaEXPB4, TaME1) were up-regulated during the first 8dc. In the less embryogenic DH31, TAA1b, GSTA2 and TaEXPB4 were already induced on 4dc. In DH25, ME was initiated quite efficiently but soon inhibited, which coincided with the lack of gene expression (TaEXPB4, TaME1) or downregulation (Tad1, XIP-R1, TaAGL14, TaNF-YA, SERK2, SERK1) on 8dc. In the recalcitrant DH50 line, the majority of genes were expressed at a lower level or not at all, indicating disturbances in ME initiation. In this study, the molecular mechanisms involved in triticale ME induction were analysed for the first time, laying the foundation for further characterisation of specific genes controlling microspore-derived embryo development.

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Section: Resultscontrasting

confidence: 70%

Changes in gene expression patterns associated with microspore embryogenesis in hexaploid triticale (×Triticosecale Wittm.)

Żur

Dubas

Krzewska

et al. 2013

Plant Cell Tiss Organ Cult

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“…Specific disease resistance induced by cold acclimation has been reported for several crops (Koike et al, 2002;Płażek et al, 2003), with some cold-induced pathogenesis-related proteins exhibiting both antifungal and antifreeze activities (Kuwabara and Imai, 2009). In particular, certain b-1,3-glucanases have been shown to be cold induced and have cryoprotective activity similar to other extracellular pathogenesisrelated proteins (Hincha et al, 1997).…”

Section: Proteins Associated With Pathogen Resistancementioning

confidence: 99%

Proteomic Study of Low-Temperature Responses in Strawberry Cultivars (Fragaria×ananassa) That Differ in Cold Tolerance

Koehler

Wilson²,

Goodpaster

et al. 2012

Plant Physiology

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To gain insight into the molecular basis contributing to overwintering hardiness, a comprehensive proteomic analysis comparing crowns of octoploid strawberry (Fragaria 3 ananassa) cultivars that differ in freezing tolerance was conducted. Four cultivars were examined for freeze tolerance and the most cold-tolerant cultivar ('Jonsok') and least-tolerant cultivar ('Frida') were compared with a goal to reveal how freezing tolerance is achieved in this distinctive overwintering structure and to identify potential cold-tolerance-associated biomarkers. Supported by univariate and multivariate analysis, a total of 63 spots from twodimensional electrophoresis analysis and 135 proteins from label-free quantitative proteomics were identified as significantly differentially expressed in crown tissue from the two strawberry cultivars exposed to 0-, 2-, and 42-d cold treatment. Proteins identified as cold-tolerance-associated included molecular chaperones, antioxidants/detoxifying enzymes, metabolic enzymes, pathogenesis-related proteins, and flavonoid pathway proteins. A number of proteins were newly identified as associated with cold tolerance. Distinctive mechanisms for cold tolerance were characterized for two cultivars. In particular, the 'Frida' cold response emphasized proteins specific to flavonoid biosynthesis, while the more freezing-tolerant 'Jonsok' had a more comprehensive suite of known stress-responsive proteins including those involved in antioxidation, detoxification, and disease resistance. The molecular basis for 'Jonsok'-enhanced cold tolerance can be explained by the constitutive level of a number of proteins that provide a physiological stress-tolerant poise. Strawberry (Fragaria 3 ananassa) cultivation predominates in regions with mild winters. In colder climates, overwintering hardiness is an essential trait for strawberry cultivation. Freezing injury of strawberry plants is one of the greatest factors in reducing crop yield and quality in temperate regions. Winter damage in Norway, for example, on average causes losses of 20%. Thus, production of cultivars with improved freezing hardiness is one of Norway's major objectives for their strawberry breeding programs. Improvement of cold hardiness is desirable for securing economic sustainability of the existing crops, and for expanding the growing regions of temperate fruit crops. Because strawberry is a representative species for the Rosaceae crops (e

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“…AMPs have been identified from a number of sources, including plants (9), insects (10), and vertebrates (11). Currently, significant interest is directed towards AMPs of endogenous origin, such as defensins, cathelicidins, and histatins (12,13).…”

Section: Introductionmentioning

confidence: 99%

Effects of linear amphiphilicity on membrane interactions of C-terminal thrombin peptides

et al. 2014

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Link to publicationCitation for published version (APA): Singh, S., Papareddy, P., Kalle, M., Schmidtchen, A., & Malmsten, M. (2014). Effects of linear amphiphilicity on membrane interactions of C-terminal thrombin peptides. RSC Advances, 4(71), 37582-37591. DOI: 10.1039/c4ra05420bGeneral rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal AbstractEffects of linear amphiphilicity on membrane interactions of antimicrobial peptides were investigated by ellipsometry, dual polarization interferometry, fluorescence spectroscopy, light scattering, and circular dichroism. In doing so, the thrombinderived GKY25 (GKYGFYTHVFRLKKWIQKVIDQFGE) was compared to WFF25(WFFFYYLIIGGGVVTHQQRKKKKDE) of identical composition, but with amino acids sorted according to hydrophobicity, the latter peptide thus displaying pronounced linear amphiphilicity. In addition, GKY25dwith identical sequence but with selected D-amino acid substitutions) was included as a control peptide, for which conformationally induced (helix-related) amphiphilicity was suppressed. Through its pronounced linear amphiphilicity, WFF25, but not the less amphiphilic GKY25 and GKY25d, forms aggregates in solution. Through its terminal W/F stretch, WFF25 also displays pronounced selectivity, with higher membrane binding and liposome rupture than GKY25 and GKY25d for anionic membranes, but suppressed peptide insertion and lytic effects for zwitterionic ones. In addition, WFF25 binds extensively to anionic polyelectrolyte components in bacterial membranes, i.e., lipopolysaccharide and lipoteichoic acid, resulting in reduced antimicrobial effects through peptide scavenging, not seen for the less amphiphilic GKY25 and GKY25d peptides. Taken together, the results thus demonstrate a series of striking effects for highly amphiphilic peptides, which need to be recognized in the development of such compounds as potential peptide therapeutics.3

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A novel plant defensin-like gene of winter wheat is specifically induced during cold acclimation

Cited by 115 publications

References 38 publications

Changes in gene expression patterns associated with microspore embryogenesis in hexaploid triticale (×Triticosecale Wittm.)

Changes in gene expression patterns associated with microspore embryogenesis in hexaploid triticale (×Triticosecale Wittm.)

Proteomic Study of Low-Temperature Responses in Strawberry Cultivars (Fragaria×ananassa) That Differ in Cold Tolerance

Effects of linear amphiphilicity on membrane interactions of C-terminal thrombin peptides

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