A Novel Peptide Isolated from a Phage Display Peptide Library Modeling Antigenic Epitope of DHAV-1 and DHAV-3

Abstract

Duck hepatitis A virus (DHAV), the major pathogen of duck virus hepatitis (DVH), causes severe diseases that threaten the duck industry worldwide. The VP1 protein, a major structural protein of DHAV, is able to induce neutralizing antibody in ducks. The purpose of this study was to identify the antigenic mimotope of DHAV by phage display technology. A monoclonal antibody (mAb) 4E6 against DHAV-1 and DHAV-3 was prepared, and a phage library prepared with the PhD-12 Phage Display Peptide Library Kit was screened with the mAb. A novel peptide, 1GLTWKLPPSM10 was identified with high affinity to the mAb and could specifically block mAb 4E6 from binding DHAV-1 and DHAV-3. Animal tests confirmed that the immunization of ducklings with the mimotope could inhibit the virus proliferation and protect the ducklings from DVH. In summary, the neutralizing conformational mimotope 1GLTWKLPPSM10 might be a promising vaccine candidate for the prevention of DHAV infection.

Integrated miRNA and mRNA Expression Profiles Reveal Differentially Expressed miR-222a as an Antiviral Factor Against Duck Hepatitis A Virus Type 1 Infection

“…Duck virus hepatitis (DVH) is a highly infectious and fast-spreading disease that has resulted in a high mortality rate among ducklings worldwide since its first isolation in 1949 ( Levine and Fabricant, 1950 ; Liu et al., 2011 ; Shi et al., 2013 ; Zhang et al., 2020 ). The causative agent of DVH is duck hepatitis A virus (DHAV)—a nonenveloped, single-stranded, and positive-sense RNA virus of the Avihepatovirus genus of the Picornaviridae family ( Delang et al., 2012 ; Mao et al., 2017 ).…”

Long Noncoding RNA Expression Rofiles Elucidate the Potential Roles of lncRNA- XR_003496198 in Duck Hepatitis A Virus Type 1 Infection

“…Duck virus hepatitis (DVH) is a highly lethal and fast-spreading disease affecting ducklings, which is caused by the duck hepatitis A virus (DHAV) (Chalmers and Woolcock, 1984;Yang et al, 2012;Zhang et al, 2020a). As the only Avihepatovirus genus in the Picornaviridae family, the DHAV genus is genetically classified into three types: DHAV-1, DHAV-2, and DHAV-3 (Tseng et al, 2007;Gao et al, 2012).…”