Integrated miRNA and mRNA Expression Profiles Reveal Differentially Expressed miR-222a as an Antiviral Factor Against Duck Hepatitis A Virus Type 1 Infection

Abstract

Duck hepatitis A virus 1 (DHAV-1) is a highly contagious etiological agent that causes acute hepatitis in young ducklings. MicroRNAs (miRNAs) play important regulatory roles in response to pathogens. However, the interplay between DHAV-1 infection and miRNAs remains ambiguous. We characterized and compared miRNA and mRNA expression profiles in duck embryo fibroblasts cells (DEFs) infected with DHAV-1. In total, 36 and 96 differentially expressed (DE) miRNAs, and 4110 and 2595 DE mRNAs, were identified at 12 and 24 h after infection. In particular, 126 and 275 miRNA–mRNA pairs with a negative correlation were chosen to construct an interaction network. Subsequently, we identified the functional annotation of DE mRNAs and target genes of DE miRNAs enriched in diverse Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, which may be important for virus resistance, cell proliferation, and metabolism. Moreover, upregulated miR-222a could negatively regulate DHAV-1 replication in DEFs and downregulate integrin subunit beta 3 (ITGB3) expression by targeting the 3′ untranslated region (3′UTR), indicating that miR-222a may modulate DHAV-1 replication via interaction with ITGB3. In conclusion, the results reveal changes of mRNAs and miRNAs during DHAV-1 infection and suggest miR-222a as an antiviral factor against DHAV-1.

Differential responses to avian pathogenic E. coli and the regulatory role of splenic miRNAs in APEC infection in Silkie chickens

“…In this study, the expression of gga-miR-221-3p was greater in the susceptible group than in the resistant group, and in combination with the above findings in humans, we speculated that gga-miR-221-3p has a proinflammatory effect on APEC infection. miR-222a, another highly expressed miRNA in the susceptible group, can act as an antiviral factor against duck hepatitis A virus 1 ( Sui et al., 2022 ). However, few other miRNAs, such as gga-miR-1663-3p, gga-miR-222b-3p, and gga-miR-7, have been reported.…”

Integrated Analysis of miRNA-mRNA Expression in Mink Lung Epithelial Cells Infected With Canine Distemper Virus

“…In recent years, high-throughput sequencing techniques were used to determine the expression profiles of miRNAs in animals, plants and viruses. Some studies showed that the integrated analysis of miRNA and mRNA in viral infection samples could confirm the regulatory mechanism of miRNA ( 28 – 30 ). The analysis of giant pandas in captivity vaccinated against CDV was operated with miRNA-mRNA.…”

mRNA and miRNA expression profiles reveal the potential roles of RLRs signaling pathway and mitophagy in duck hepatitis A virus type 1 infection

“…For example, researchers utilized lncRNA sequencing and RNA sequencing ( RNA-seq ) to examine genetic alterations in DHAV-1-infected duck embryo fibroblast ( DEF ) cells ( Sui et al, 2022 ). Additionally, a study elucidated mRNA and miRNA profiles in DHAV-1-infected duck embryo fibroblast ( DEF ) cells through RNA-seq and miRNA sequencing ( miRNA-seq ) ( Sui et al, 2021 ). Furthermore, mRNA expression profiles were analyzed in duck liver infected with DHAV-3 ( Cao et al, 2020 ).…”