A novel pathogenesis-related protein (SsPR10) from Solanum surattense with ribonucleolytic and antimicrobial activity is stress- and pathogen-inducible

Liu, Xiaojun; Huang, Beibei; Lin, Juan; Fei, Jiong; Chen, Zhonghai; Pang, Yongzhen; Sun, Xiaofen; Tang, Kexuan

doi:10.1016/j.jplph.2005.04.031

Cited by 88 publications

(44 citation statements)

References 37 publications

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“…The functions of PR-10s are currently not clear, but they are induced by various factors such as development and stresses in Solanum sp. [17]. Small heat shock proteins also showed a distinct pattern of abundance, suggesting a potentially important role during tuber development and maturation.…”

Section: Generalmentioning

confidence: 98%

Proteomic analysis of the potato tuber life cycle

et al. 2006

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The tuber of potato (Solanum tuberosum) is commonly used as a model for underground storage organs. In this study, changes in the proteome were followed from tuberization, through tuber development and storage into the sprouting phase. Data interrogation using principal component analysis was able to clearly discriminate between the various stages of the tuber life cycle. Moreover, five well-defined protein expression patterns were found by hierarchical clustering. Altogether 150 proteins showing highly significant differences in abundance between specific stages in the life cycle were highlighted; 59 of these were identified. In addition, 50 proteins with smaller changes in abundance were identified, including several novel proteins. Most noticeably, the development process was characterized by the accumulation of the major storage protein patatin isoforms and enzymes involved in disease and defense reactions. Furthermore, enzymes involved in carbohydrate and energy metabolism and protein processing were associated with development but decreased during tuber maturation. These results represent the first comprehensive picture of many proteins involved in the tuber development and physiology.

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Section: Generalmentioning

confidence: 98%

Proteomic analysis of the potato tuber life cycle

et al. 2006

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“…15) AhSIPR10 expression was also responsive towards defense/stress signaling SA, and H 2 O 2 treatments. 37) In our experiment, SoPR10 was also induced by SA and H 2 O 2 .…”

Section: The Expression Of Sopr10 Under Other Abiotic Stressesmentioning

confidence: 96%

“…17) It has been reported that, PR-10 proteins were developmentally regulated and constitutively accumulated in different plant tissues and organs. 15) Extensive analysis of JIOsPR10 in mature tissues of rice revealed a constitutive expression pattern with high levels detected in roots. Pulla et al found PgPR10-2 was also constitutively expressed in root, leaves, and shoot with highest transcripts in root.…”

Section: The Expression Of Sopr10 Under Other Abiotic Stressesmentioning

confidence: 99%

“…4) So far, there are many reports of cloning, expression, and characterization of multiple PR-10 or PR-10-like proteins from plants. More than 100 PR-10 and PR-10-related sequences have been identified in numerous plants including pea, 5) white pine, 6) bean, 7) lupine, 8) white spruce, 9) rice, 10) plum, 11) grapevine, 12) soybean, 13) potato, 14) Solanum surattense, 15) Panax ginseng, 16) and Zea mays. 17) Most PR-10 proteins were known to be induced by various biotic and abiotic stresses, and constitutive expression of these proteins was also reported.…”

mentioning

confidence: 99%

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Molecular cloning and characterization of pathogenesis-related protein family 10 gene from spinach (SoPR10)

Bai

Long

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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PR10 genes encode small, intracellular proteins that respond to biotic and abiotic stresses. In this study, a cDNA clone (designated as SoPR10, GenBank Accession No. KC142174) encoding a PR10 protein from spinach (Spinacia oleracea L.) was isolated and characterized. SoPR10 encoded a 161-amino acid polypeptide with a predicted molecular mass of 19.76 kDa and a pI of 4.61. Real-time quantitative analysis indicated that SoPR10 was constitutively expressed in root and shoot. The abundance of SoPR10 in salt-resistant cultivar (Chaoji) was generally greater than in salt-sensitive cultivar (Daye) under 160 mM L−1 treatment for 0.5, 3, and 6 h. The expression of SoPR10 was also induced by other abiotic stresses including polyethylene glycol, NaCl, salicylic acid, and H2O2. Our results indicated that SoPR10 might play important roles under nitrate stress and other abiotic stresses.

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“…Members of this family are widely utilized as important sources of food and medicine. For instance, S. surattense (Berkateli, Kantakari) has been reported to exhibit antimicrobial (Liu et al, 2006), antifungal (Sheeba & Palanivel, 2013), antioxidant (Muruhan et al, 2013), antidiabetic (Gupta et al, 2011) and antimalarial (Ramazani et al, 2010) properties. Certain species of this family are rich in alkaloids and are therefore toxic in nature.…”

Section: Introductionmentioning

confidence: 99%

Purification, identification and preliminary crystallographic studies of an allergenic protein fromSolanum melongena

Jain

Salunke

2015

Acta Cryst Sect F

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Solanum melongena (eggplant), a member of the Solanaceae family, is a widely cultivated vegetable crop and is commonly used as a food throughout the world. Allergic reactions caused by members of this family are well known. However, mechanistic analyses to understand their molecular basis have not been adequately explored. In order to address this issue, the 7S vicilin protein (SM80.1) of size 45 kDa was purified from seeds of S. melongena by ammonium sulfate fractionation and size-exclusion chromatography. Significant homology of SM80.1 to an allergy-related protein from S. lycopersicum was identified through a BLAST search. Crystallization attempts with purified protein using the hanging-drop vapour-diffusion method led to hexagonal-shaped crystals. The crystals diffracted to 2.21 Å resolution and belonged to space group P6 3 22, with unit-cell parameters a = 117.9, c = 123.5 Å .

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A novel pathogenesis-related protein (SsPR10) from Solanum surattense with ribonucleolytic and antimicrobial activity is stress- and pathogen-inducible

Cited by 88 publications

References 37 publications

Proteomic analysis of the potato tuber life cycle

Proteomic analysis of the potato tuber life cycle

Molecular cloning and characterization of pathogenesis-related protein family 10 gene from spinach (SoPR10)

Purification, identification and preliminary crystallographic studies of an allergenic protein fromSolanum melongena

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