A novel paper-plastic hybrid device for the simultaneous loop-mediated isothermal amplification and detection of DNA

Kim, Jae Heon; Yoo, In Sang; An, Jeong Ho; Kim, Sang-Hyo

doi:10.1016/j.matlet.2017.12.030

Cited by 17 publications

(2 citation statements)

References 15 publications

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“…using the reported method is 2.8 × 10 −5 ng μL −1 . Compared with previously reported on-chip LAMP protocols, the reported method exhibited a comparable sensitivity (Table 1 ) [ 25 , 39 – 45 ], and non-negligible advantages of ease-of-use and cost-effectiveness. Correlated well with LAMP reaction performed inside tube, LAMP reaction performed on chip still exhibits good specificity.…”

Section: Resultsmentioning

confidence: 90%

Fluorescent on-site detection of multiple pathogens using smartphone-based portable device with paper-based isothermal amplification chip

Zhou

Pan

et al. 2022

Microchim Acta

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The development of cost-effective, portable, and ease-of-use sensing system for on-site genetic diagnostics is highly desirable for pathogen screening and infectious disease diagnosis. This study develops (1) a paper-based biochip which is able to integrate the loop-mediated isothermal amplification (LAMP) protocols for simultaneous detection of Escherichia coli O157:H7, Salmonella spp., and Staphylococcus aureus , and (2) a stand-alone smartphone-based portable device which can control exactly 65 °C for isothermal amplification as well as collect and analyze the thus generated fluorescence signals. The reported sensing system has been successfully demonstrated for foodborne pathogen detection with a limit of detection of 2.8 × 10 −5 ng μL −1 . Spiked milk samples with concentration as low as 10 CFU mL −1 were successfully determined within 4 h, demonstrating the practicality of the reported sensing system in the fields. The reported sensing system featuring simplicity and reliability is ideally suited for genetic diagnostics in low resource settings. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00604-022-05419-x.

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Section: Resultsmentioning

confidence: 90%

Fluorescent on-site detection of multiple pathogens using smartphone-based portable device with paper-based isothermal amplification chip

Zhou

Pan

et al. 2022

Microchim Acta

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“…Further, the proposed assay can detect DNA concentrations lower than other POC devices based on LAMP used for the detection of other analytical targets (such as bacteria). These comparisons applies to those paper-based devices using HNB fluorescence [ 10 ] or color [ 44 ] change detection, and lateral flow devices [ 7 ]. In fact, in previous works, HNB fluorescence change detection of the positive LAMP reaction in paper-based devices could detect 0.7 pg (4.1 x 10 2 copies) of Streptococcus pneumoniae genomic DNA [ 10 ], while a paper-plastic hybrid device detected 18 fg of Staphylococcus aureus DNA with HNB color change detection by the naked eye [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Towards a versatile and economic Chagas Disease point-of-care testing system, by integrating loop-mediated isothermal amplification and contactless/label-free conductivity detection

et al. 2021

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Rapid diagnosis by using small, simple, and portable devices could represent one of the best strategies to limit the damage and contain the spread of viral, bacterial or protozoa diseases, principally when they can be transmitted by air and are highly contagious, as some respiratory viruses are. The presence of antibodies in blood or serum samples is not the best option for deciding when a person must be quarantined to stop transmission of disease, given that cured patients have antibodies, so the best diagnosis methods rely on the use of nucleic acid amplification procedures. Here we present a very simple device and detection principle, based a paper discs coupled to contactless conductivity (C4D) sensors, can provide fast and easy diagnostics that are needed when an epidemic outbreak develops. The paper device presented here solves one of the main drawbacks that nucleic acid amplification tests have when they are performed outside of central laboratories. As the device is sealed before amplification and integrally disposed in this way, amplimers release cannot occur, allowing repetitive testing in the physician’s practice, ambulances, or other places that are not prepared to avoid cross-contamination of new samples. The use of very low volume samples allows efficient reagent use and the development of low cost, simple, and disposable point-of-care diagnostic systems.

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Paper-Based Molecular Diagnostics

2020

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A novel paper-plastic hybrid device for the simultaneous loop-mediated isothermal amplification and detection of DNA

Cited by 17 publications

References 15 publications

Fluorescent on-site detection of multiple pathogens using smartphone-based portable device with paper-based isothermal amplification chip

Fluorescent on-site detection of multiple pathogens using smartphone-based portable device with paper-based isothermal amplification chip

Towards a versatile and economic Chagas Disease point-of-care testing system, by integrating loop-mediated isothermal amplification and contactless/label-free conductivity detection

Paper-Based Molecular Diagnostics

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