A novel panel of serum miR-21/miR-155/miR-365 as a potential diagnostic biomarker for breast cancer

Han, J; Jiang, Yong; Zhang, Chun Hui; Yang, Yan; Pang, Da; Song, Yan; Zhang, Guo Qiang

doi:10.4174/astr.2017.92.2.55

Cited by 96 publications

(71 citation statements)

References 29 publications

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“…miR-21 has been frequently reported to be aberrantly overexpressed in diverse tumors, including glioblastoma, breast cancer and malignant cholangiocytes (22,23). Recently, miR-21-5p was also reported to be overexpressed in gastric cancer (24).…”

Section: Discussionmentioning

confidence: 99%

miR‑21‑5p targets PDHA1 to regulate glycolysis and cancer progression in gastric cancer

Liu

Fei

et al. 2018

Oncol Rep

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Pyruvate dehydrogenase A1 (PDHA1) is a component of the pyruvate dehydrogenase enzyme complex, which links glycolysis and the tricarboxylic acid cycle, and is important for cancer metabolism shift. PDHA1 downregulation has been revealed in several types of cancer to enhance glycolysis. However, the role of PDHA1 in gastric cancer remains largely unknown. In the present study, we found that PDHA1 was significantly downregulated in gastric cancer, and associated with poor prognosis. PDHA1 downregulation promoted gastric cancer glycolysis and cancer progression. miR‑21‑5p directly targeted PDHA1 to suppress PDHA1 expression, and promote glycolysis as well as cell proliferation in gastric cancer. Moreover, miR‑21‑5p was significantly upregulated in gastric cancer and negatively associated with PDHA1 expression in gastric cancer samples. Our results indicated that miR‑21‑5p targeted PDHA1 to regulate a metabolic switch and cancer progression in gastric cancer, and reveal the potential role of the miR‑21‑5p/PDHA1 axis in gastric cancer treatment.

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Section: Discussionmentioning

confidence: 99%

miR‑21‑5p targets PDHA1 to regulate glycolysis and cancer progression in gastric cancer

Liu

Fei

et al. 2018

Oncol Rep

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“…In breast cancers, miR10b, miR125b and miR145 are consistently found to be down-regulated and miR21, miR155 are consistently up-regulated [41,42]. It was also reported that miRNAs can still be detected from formalin fixed and paraffin embedded tissue samples, collected blood and serum too.…”

Section: Methods To Detect Mirnas In Breast Cancermentioning

confidence: 97%

The roles of miRNAs in human breast cancer and canine mammary tumor

Cheah

2017

Appl Cancer Res

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MicroRNAs have become a hot topic in cancer research nowadays due to their important role not only on cancer development, progression, invasion but also on repression of cancer related genes. With advanced technologies, these microRNAs can easily be detected from biopsy samples and blood for early diagnosis, prognosis and treatment. Due to increasing demand of research in exploring expression profile of microRNAs with respect to different subtypes of breast cancer, this review aimed to provide an update on microRNA database available resources, canine breast cancer models, the role of microRNA as oncomir or oncosupressor, detection of microRNAs and potential of miRNAs for breast cancer treatment.

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“…Furthermore, miR-365-3p has been reported to serve as a therapeutic biomarker for various cancers and tumors such as lung cancer [39,40], colon cancer [28], pancreatic cancer [41], breast cancer [42] and gastric tumorigenesis [43]. Moreover, miR-365-3p inhibited vascular smooth muscle cell proliferation through targeting of CCND1 [44].…”

Section: The Expression Pro Le and Functional Roles Of Bta-mir-365-3pmentioning

confidence: 99%

MicroRNA bta-miR-365-3p inhibits proliferation but promotes differentiation of primary bovine myoblasts by targeting the activin A receptor type I

Hao¹,

Wang

et al. 2020

Preprint

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Background: MicroRNAs act as post-transcriptional regulators that repress translation or degrades mRNA transcripts. Each microRNA has many mRNA targets and each mRNA may be targeted by several microRNAs. Skeletal muscles express a plethora of microRNA genes that regulate muscle development and function by controlling the expression of protein-coding target genes. To expand our understanding of the role of microRNA, specifically bta-miR-365-3p, in muscle biology, we studied its function in regulating primary bovine myoblast proliferation and differentiation.Results: We first show that bta-miR-365-3p is predominantly expressed in skeletal muscle and heart tissue in Chinese Qinchuan beef cattle. Quantitative PCR and western blotting showed that overexpression of bta-miR-365-3p significantly reduced the levels of cyclinD1 (CCND1), cyclin dependent kinase 2 (CDK2) and proliferating cell nuclear antigen (PCNA) but stimulated the expression of muscle differentiation markers MYOD1, MYOG, both at mRNA and protein level. Moreover, downregulation of bta-miR-365-3p increased the expression of CCND1, CDK2 and PCNA but decreased the expression of MYOD1 and MYOG at both mRNA and protein levels. Furthermore, flow cytometry, EdU proliferation assays and immunostaining showed that increased levels of bta-miR-365-3p suppressed cell proliferation but promoted myotube formation, whereas a decreased level of bta-miR-365-3p had opposite consequences. Finally, we determined that activin A receptor type I (ACVR1) is a direct target of bta-miR-365-3p. Thus, dual luciferase gene reporter assays demonstrated that bta-miR-365-3p can bind to the 3'UTR of ACVR1 gene to regulate its expression. Consistently, knock-down of ACVR1 was associated with reduced CDK2, CCND1 and PCNA expression but increased MYOG and MYOD1 expression both at mRNA and protein level.Conclusion: Collectively these data suggest that bta-miR-365-3p represses proliferation but promotes differentiation of bovine myoblasts through a mechanism involving downregulation of ACVR1.

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A novel panel of serum miR-21/miR-155/miR-365 as a potential diagnostic biomarker for breast cancer

Cited by 96 publications

References 29 publications

miR‑21‑5p targets PDHA1 to regulate glycolysis and cancer progression in gastric cancer

miR‑21‑5p targets PDHA1 to regulate glycolysis and cancer progression in gastric cancer

The roles of miRNAs in human breast cancer and canine mammary tumor

MicroRNA bta-miR-365-3p inhibits proliferation but promotes differentiation of primary bovine myoblasts by targeting the activin A receptor type I

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