A novel p.K116Q SNP in the OLR1 gene and its relation to fecundity in Awassi ewes

Mohammed, Mohammed M.; Al‐Shuhaib, Mohammed Baqur S.; Al-Shuhaib, Mohammed Baqur S.

doi:10.1016/j.theriogenology.2022.03.014

Cited by 13 publications

(6 citation statements)

References 45 publications

(27 reference statements)

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“… 14 Despite their ability to cope with harsh conditions in unfavorable situations, 15 Awassi sheep have been reported to be less prolific than Karakul and Assaf sheep in the Middle East. 16 Most breeders in the Middle East are concerned about Awassi sheep’s low reproductive performance, which drives efforts to improve their reproductive quality. The FST gene may be useful in improving breeding efficiency in Awassi sheep.…”

Section: Introductionmentioning

confidence: 99%

A Novel c.100C > G Mutation in the FST Gene and Its Relation With the Reproductive Traits of Awassi Ewes

Albazi

Al‐Shuhaib

Merzah

et al. 2023

Bioinform Biol Insights

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Reproductive traits are affected by many factors, including ovarian function, hormones, and genetics. Genetic polymorphisms of candidate genes are associated with reproductive traits. Several candidate genes are associated with economic traits, including the follistatin ( FST) gene. Thus, this study aimed to evaluate whether the genetic variations in the FST gene are associated with the reproductive traits in Awassi ewes. The genomic DNA was extracted from 109 twin ewes and 123 single-progeny ewes. Therefore, 4 sequence fragments from the FST gene were amplified using polymerase chain reaction (PCR) (exon 2/240, exon 3/268, exon 4/254, and exon 5/266 bp, respectively). For a 254 bp amplicon, 3 genotypes were identified: CC, CG, and GG. Sequencing revealed a novel mutation in CG genotypes c.100C > G. The statistical analysis of c.100C > G showed an association with reproductive characteristics. Ewes carrying the c.100C > G had significantly ( P ⩽ .01) lower litter sizes, twinning rates, lambing rates, and more days to lambing compared with CG and CC genotypes. Logistic regression analysis confirmed that the c.100C > G single-nucleotide polymorphism (SNP) is responsible for decreasing litter size. According to these results, the variant c.100C > G negatively affects the traits of interest and is associated with lower reproductive traits in Awassi sheep. As a result of this study, ewes carrying the c.100C > G SNP have lower litter size and are less prolific.

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Section: Introductionmentioning

confidence: 99%

A Novel c.100C > G Mutation in the FST Gene and Its Relation With the Reproductive Traits of Awassi Ewes

Albazi

Al‐Shuhaib

Merzah

et al. 2023

Bioinform Biol Insights

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“…The genetic improvement by traditional breeding methods is restricted for quantitative traits. Scientists can identify genes that impact economically relevant characteristics and include them in genomic selection studies, thanks to advancements in molecular technology (Cesarani et al, 2019;Gorlov et al, 2020;Mohammed et al, 2022). In this regard, molecular studies play a critical role in animal breeding.…”

Section: Introductionmentioning

confidence: 99%

Akkaraman Koyun Irkında Callipyge Gen Polimorfizmin Araştırılması

KAVUZKOZ

Kirikçi

2023

Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Callipyge is a candidate gene with a significant effect on meat quality and growth in sheep. The aim of this study is to investigate the polymorphism of the Callipyge gene (CLPG) in Akkaraman breed. In the study, DNAs obtained from blood samples of 50 Akkaraman sheep were used as study material. The CLPG/Faq1 polymorphism was examined in all individuals using the method PCR-RFLP. Only A allele and AA genotype were detected after PCR-RFLP procedure. This is the first study in which Callipyge gene polymorphism was investigated in Akkaraman breed, and the findings show that Akkaraman breed has a monomorphic structure in terms of Callipyge/Faq1 polymorphism.

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“… 3 Identifying the genetic mechanisms responsible for lamb reproductive performance is imperative to identify the candidate genes and their causal mutations. 4 , 5 The gremlin 1 (GREM1) gene, 6 the prolactin gene, 7 and the oxidized low density lipoprotein receptor 1 (OLR1) gene 8 have all been found to stimulate reproductive function in Awassi sheep. Another gene, paired-like homeodomain transcription factor 2 ( PITX2 ), has also been found to play a role in livestock reproduction.…”

Section: Introductionmentioning

confidence: 99%

Association of Novel C319T Variant of PITX2 Gene 3’UTR Region With Reproductive Performance in Awassi Sheep

Al‐Shuhaib

Khazaal

2023

Bioinform Biol Insights

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Several genes influence sheep’s reproductive performance, among them the paired-like homeodomain transcription factor 2 ( PITX2) gene. Thus, this study aimed to examine whether the variability within the PITX2 gene is associated with the reproductive performance of Awassi ewes. A total of 123 single-progeny ewes and 109 twin ewes were used to extract genomic DNA. An amplicon of 4 sequence fragments from exons 2, 4, 5 (upstream portion), and 5 (downstream portion) of the PITX2 gene was generated by polymerase chain reaction (PCR), 228, 304, 381, and 382 bp, respectively. Three genotypes of 382 bp amplicons were identified: CC, CT, and TT. Sequence analysis revealed a novel mutation in the CT genotype 319C > T. Statistical analysis revealed that single-nucleotide polymorphism (SNP) 319C > T was associated with reproductive performance. Single-nucleotide polymorphism 319C > T-carrying ewes had significantly ( P ⩽ .01) lower litter sizes, twinning rates, lambing rates, and more days to lambing than those carrying CT and CC genotypes. Based on a logistic regression analysis, it was confirmed that the 319C > T SNP decreased litter size. Ewes with TT genotype produced fewer lambs than ewes with CT and CC genotypes. According to these results, the variant 319C> T SNP negatively affects the reproductive performance of Awassi sheep. Ewes carrying the 319C > T SNP have a lower litter size and are less prolific than those without the SNP.

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A novel p.K116Q SNP in the OLR1 gene and its relation to fecundity in Awassi ewes

Cited by 13 publications

References 45 publications

A Novel c.100C > G Mutation in the FST Gene and Its Relation With the Reproductive Traits of Awassi Ewes

A Novel c.100C > G Mutation in the FST Gene and Its Relation With the Reproductive Traits of Awassi Ewes

Akkaraman Koyun Irkında Callipyge Gen Polimorfizmin Araştırılması

Association of Novel C319T Variant of PITX2 Gene 3’UTR Region With Reproductive Performance in Awassi Sheep

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