A novel osteopontin‐like protein is expressed in the trout ovary during ovulation

Bobe, Julien; Goetz, Frederick W.

doi:10.1016/s0014-5793(01)02090-7

Cited by 20 publications

(18 citation statements)

References 29 publications

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“…kallikrein, KT-14; trout ovulatory protein-2, TOP-2; and novel osteopontin-like protein, NOP) [28-30]. The expression of this set of genes was significantly (p < 0.05) increased over control in response to rtTNFα treatment (100 ng/ml).…”

Section: Resultsmentioning

confidence: 99%

Cellular and molecular evidence for a role of tumor necrosis factor alpha in the ovulatory mechanism of trout

Crespo

Bonnet²,

Roher

et al. 2010

Reprod Biol Endocrinol

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BackgroundThe relevance of immune-endocrine interactions to the regulation of ovarian function in teleosts is virtually unexplored. As part of the innate immune response during infection, a number of cytokines such as tumor necrosis factor alpha (TNF alpha) and other immune factors, are produced and act on the reproductive system. However, TNF alpha is also an important physiological player in the ovulatory process in mammals. In the present study, we have examined for the first time the effects of TNF alpha in vitro in preovulatory ovarian follicles of a teleost fish, the brown trout (Salmo trutta).MethodsTo determine the in vivo regulation of TNF alpha expression in the ovary, preovulatory brook trout (Salvelinus fontinalis) were injected intraperitoneally with either saline or bacterial lipopolysaccharide (LPS). In control and recombinant trout TNF alpha (rtTNF alpha)-treated brown trout granulosa cells, we examined the percentage of apoptosis by flow cytometry analysis and cell viability by propidium iodide (PI) staining. Furthermore, we determined the in vitro effects of rtTNF alpha on follicle contraction and testosterone production in preovulatory brown trout ovarian follicles. In addition, we analyzed the gene expression profiles of control and rtTNF alpha-treated ovarian tissue by microarray and real-time PCR (qPCR) analyses.ResultsLPS administration in vivo causes a significant induction of the ovarian expression of TNF alpha. Treatment with rtTNF alpha induces granulosa cell apoptosis, decreases granulosa cell viability and stimulates the expression of genes known to be involved in the normal ovulatory process in trout. In addition, rtTNF alpha causes a significant increase in follicle contraction and testosterone production. Also, using a salmonid-specific microarray platform (SFA2.0 immunochip) we observed that rtTNF alpha induces the expression of genes known to be involved in inflammation, proteolysis and tissue remodeling. Furthermore, the expression of kallikrein, TOP-2, serine protease 23 and ADAM 22, genes that have been postulated to be involved in proteolytic and tissue remodeling processes during ovulation in trout, increases in follicles incubated in the presence of rtTNF alpha.ConclusionsIn view of these results, we propose that TNF alpha could have an important role in the biomechanics of follicle weakening, ovarian rupture and oocyte expulsion during ovulation in trout, primarily through its stimulation of follicular cell apoptosis and the expression of genes involved in follicle wall proteolysis and contraction.

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Section: Resultsmentioning

confidence: 99%

Cellular and molecular evidence for a role of tumor necrosis factor alpha in the ovulatory mechanism of trout

Crespo

Bonnet²,

Roher

et al. 2010

Reprod Biol Endocrinol

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“…An SPP1 ortholog has been identified in the zebrafish and trout (Bobe and Goetz, 2001;Kawasaki et al, 2004), as well as in fugu, medaka, stickleback, sea bream, and fathead minnow (Fonseca et al, 2007). Although SPP1 and the ancestral SPARCL1 remain in a linked chromosomal region in tetrapod genomes, these 2 genes appear to be separated by an interchromosomal re-arrangement in these teleost fish (Fig.…”

Section: (B) Scpp Evolution By Gene Gain-and-lossmentioning

confidence: 99%

SCPP Gene Evolution and the Dental Mineralization Continuum

Kawasaki

Weiss

2008

J Dent Res

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Many genes critical to vertebrate skeletal mineralization are members of the secretory calcium-binding phosphoprotein (SCPP) gene family, which has evolved by gene duplication from a single ancestral gene. In humans, mutations in some of these SCPP genes have been associated with various diseases related to dentin or enamel hypoplasia. Recently, systematic searches for SCPP genes of various species have allowed us to investigate the history of phylogenetically variable dental tissues as a whole. One important conclusion is that not all disease-associated SCPP genes are present in tetrapods, and teleost fish probably have none, even in toothed species, having acquired their complement of SCPP genes through an independent duplication history. Here, we review comparative analyses of mineralized dental tissues, with particular emphasis on the use of SCPPs, within and between tetrapods and teleosts. Current knowledge suggests a close relationship among bone, dentin, teleost fish enameloid (enamel-like hard tissue), and tetrapod enamel. These tissues thus form a mineralized-tissue continuum. Contemporary dental tissues have evolved from an ancestral continuum through lineage-specific modifications.

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“…Hybridizations were carried out at 60°C overnight followed by stringent washing conditions (13). Brook trout Northern blots were hybridized as previously described (19). Images were captured using the Storm 840 PhosphorImager system equipped with the ImageQuant data reduction system (Molecular Dynamics, Sunnyvale, CA).…”

Section: Isolation and Characterization Of Chicken And Trout Dr6 Cdnasmentioning

confidence: 99%

Conservation of Death Receptor-6 in Avian and Piscine Vertebrates

Bridgham

Bobe²,

Goetz

et al. 2001

Biochemical and Biophysical Research Communications

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A novel osteopontin‐like protein is expressed in the trout ovary during ovulation

Cited by 20 publications

References 29 publications

Cellular and molecular evidence for a role of tumor necrosis factor alpha in the ovulatory mechanism of trout

Cellular and molecular evidence for a role of tumor necrosis factor alpha in the ovulatory mechanism of trout

SCPP Gene Evolution and the Dental Mineralization Continuum

Conservation of Death Receptor-6 in Avian and Piscine Vertebrates

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