A novel optimised and validated method for analysis of multi-residues of pesticides in fruits and vegetables by microwave-assisted extraction (MAE)–dispersive solid-phase extraction (d-SPE)–retention time locked (RTL)–gas chromatography–mass spectrometry with Deconvolution reporting software (DRS)

Satpathy, Gouri; Tyagi, Yogesh Kumar; Gupta, Rajinder K.

doi:10.1016/j.foodchem.2011.01.087

Cited by 44 publications

(25 citation statements)

References 20 publications

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“…In this case, the surrounding solvent can have a low dielectric constant and thus remains cold during extraction. This method can be used to extract thermo-sensitive compounds like pigments (Satpathy et al 2011).…”

Section: Microwave-assisted Extractionmentioning

confidence: 99%

Functional properties of grape (<italic>Vitis vinifera</italic>) seed extract and possible extraction techniques -A review

Krithika

Naik

Pragalyaashree

2015

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Grape (Vitis vinifera) member of family vitaceae, is an excellent source of phenolics, particulary in the skin and seeds. Phenolic compounds have attracted much interest recently, because they are potent antioxidant and exhibit various other physiological activities including anticarcinogenic, antimicrobial, anti-inflammatory and antihypertensive activities associated with reduced risk of chronic diseases like cardiovascular diseases. The method of processing and extraction play a vital role in the quality of nutraceuticals extracted. The traditional extraction techniques are time-consuming and require relatively large quantities of solvents. In view of increased awareness for health related compounds from plant materials, there is an increasing demand for better extraction techniques which are nontoxic, environmentally safe and inexpensive. This review paper discusses briefly on grape phenolics, important nutraceuticals extracted from grape seeds and its positive health effects. The possibility of application of novel extraction methods like sonication assisted extraction, microwave assisted extraction techniques and supercritical fluid extractions for efficient extraction of plant are discussed.

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Section: Microwave-assisted Extractionmentioning

confidence: 99%

Functional properties of grape (<italic>Vitis vinifera</italic>) seed extract and possible extraction techniques -A review

Krithika

Naik

Pragalyaashree

2015

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“…A modified QuEChERS method using ethyl acetate was used for the preparation of samples [9,11,12]. According to this method, 10 g of finely ground sub-sample was placed in a polypropylene centrifuge tube (50 mL).…”

Section: 2sample Preparationmentioning

confidence: 99%

“…Direct analysis of acetonitrile by GC is not compatible and can degrade the GC column phase. It also leads to poor focusing of chromatographic peaks because the vapour created by the sample overloads the insert liner dimension due to a high thermal expansion coefficient [10,11]. Moreover, evaporation and reconstitution of acetonitrile can cause loss of concentration of some of organophosphorous analytes such as monocrotophos, phorate, etc.…”

mentioning

confidence: 99%

Development and Validation of Multi-Residue Analysis of 82 Pesticides in Grapes and Pomegranate as per the Requirements of the European Union (EU) and Codex Alimentarius Using GC-MS/MS with Compound Based Screening

Tyagi¹,

Gupta²

2014

AJFST

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The Quick Easy Cheap Effective Rugged and Safe (QuEChERS) multiresidue method has been validated for the extraction of 82 pesticides belonging to various chemical classes from grapes and pomegranate (commodities with high sugar and low lipid contents). A mixture of 82 pesticides amenable to gas chromatography (GC) was quantitatively recovered from spiked grapes and pomegranate and determined using gas chromatographytandem mass spectrometry (GC-MS/MS). The method employed involved initial extraction in a water/ethyl acetate system, an extraction/partitioning step after the addition of salt, and a cleanup step utilizing dispersive solid-phase extraction (d-SPE); this combination ensured that it was a rapid, simple and cost-effective procedure. The method setup is streamlined with the new software approach of Compound Based Scanning (CBS). The matrix-matched calibration results have demonstrated good reproducibility, robustness and linearity. The spiking levels for the recovery experiments were 0.005, 0.01 and 0.1 mg kg -1 for GC-MS/MS analyses. Adequate pesticide quantification and identity confirmation were attained, even at the lowest concentration levels, considering the high signal-to-noise ratios, the very good accuracies and precisions, as well as the good matches between the observed ion ratios. Mean recoveries mostly ranged between 70 and 110 % (91% on average), and RSD were generally below 12% (7.3% on average). The use of analyte protectants during GC analysis was demonstrated to provide a good alternative to the use of matrix-matched standards to minimize matrix-effect-related errors. For all compounds LODs were 0.001 to 0.005 mgkg -1 and LOQs were 0.005 to 0.020 mgkg -1. Correlation coefficients of the calibration curves were >0.991. Based on these results, the methodology has been proven to be highly efficient and robust and thus suitable for monitoring the Maximum Residual Limit (MRL) compliance of a wide range of commodity/pesticide combinations.

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“…Nesta técnica, a amostra é imersa num frasco aberto ou fechado contendo solvente e é irradiada com micro-ondas. A principal limitação da técnica é o uso de solventes que absorvam energia de micro-ondas, pois solventes não polares não absorvem essa energia [26,27] . Além disso, em geral, é necessária uma etapa de limpeza do extrato [28] .…”

Section: Extração Em Fase Sólida Dispersivaunclassified

“…Os limites de detecção obtidos estavam entre 2 e 5 µg kg -1 e os limites de quantificação entre 7 e 18 µg kg -1 . Satpathy et al [26] desenvolveram um método multirresíduo para análise de 72 agrotóxicos em frutas e vegetais empregando extração por MAE, limpeza do extrato por D-SPE seguida de determinação por GC-MS. Os resultados apresentaram boa linearidade, robustez, precisão e reprodutibilidade. Esta combinação permite que método QuEChERS empregando nanotubos de carbono de paredes múltiplas como sorvente na etapa de D-SPE.…”

Section: Outros Sorventesunclassified

Extração em Fase Sólida Dispersiva na determinação de resíduos e contaminantes em alimentos

Cabrera¹,

Martins²,

Prímel³

et al. 2012

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ResumoEste artigo proporciona uma visão geral da técnica de Extração em Fase Sólida Dispersiva (D-SPE) como uma importante etapa do método QuEChERS que, com um procedimento fácil e rápido, permite obter extratos mais limpos para as análises multirresíduo. A D-SPE envolve, em geral, o uso de um ou mais sorventes, juntamente com sulfato de magnésio anidro, em um tubo de centrífuga, para remover os coextrativos do extrato orgânico. Esta técnica é prática e eficiente para limpar extratos de diferentes amostras complexas, como alimentos de origem vegetal ou animal. Alguns detalhes práticos do uso de diferentes sorventes são apresentados com as principais aplicações. Palavras-chavePreparo de amostra; limpeza de extratos; sorventes. Dispersive Solid Phase Extraction in the determination of residues and contaminants in food AbstractThis paper provide an overview of the technique Dispersive Solid Phase Extraction (D-SPE) as an important step of the QuEChERS method that permit to obtain, in a easy and quick procedure, cleaned extracts for the multiresidue analysis. The D-SPE involves, in general, the use of one or more sorbent together with magnesium sulfate anhydrous in a centrifuge tube to remove the co-extractives from the organic extract. This technique is practical and efficient to clean-up extracts from different complex samples, like foods of vegetable or animal origin. Some practical details of different sorbents are presented with principal applications.

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Cited by 44 publications

References 20 publications

Functional properties of grape (<italic>Vitis vinifera</italic>) seed extract and possible extraction techniques -A review

Functional properties of grape (<italic>Vitis vinifera</italic>) seed extract and possible extraction techniques -A review

Development and Validation of Multi-Residue Analysis of 82 Pesticides in Grapes and Pomegranate as per the Requirements of the European Union (EU) and Codex Alimentarius Using GC-MS/MS with Compound Based Screening

Extração em Fase Sólida Dispersiva na determinação de resíduos e contaminantes em alimentos

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