A novel nonreceptor tyrosine kinase, Srm: cloning and targeted disruption.

Kohmura, Naohiro; Yagi, Takeshi; Tomooka, Yasuhiro; Oyanagi, Mitsuru; Kominami, Ryo; Takeda, Nobuo; Chiba, Joe; Ikawa, Yoji; Aizawa, Shinichi

doi:10.1128/mcb.14.10.6915

Cited by 48 publications

(42 citation statements)

References 74 publications

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“…Culture and transfection of TT2 (29) embryonic stem (ES) cells were done as previously described (14,16,31). Transfected ES cells were selected in medium containing G418 (200 g/ml) and ganciclovir (2 M).…”

Section: Methodsmentioning

confidence: 99%

Immunocytochemical Analyses and Targeted Gene Disruption of GTPBP1

Senju

Iyama²,

Kaneko

et al. 2000

Molecular and Cellular Biology

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We previously identified a gene encoding a putative GTPase, GTPBP1, which is structurally related to elongation factor 1␣, a key component of protein biosynthesis machinery. The primary structure of GTPBP1 is highly conserved between human and mouse (97% identical at the amino acid level). Expression of this gene is enhanced by gamma interferon in a monocytic cell line, THP-1. Although counterparts of this molecule in Caenorhabditis elegans and Ascaris suum have also been identified, the function of this molecule remains to be clarified. In the present study, our immunohistochemical analyses on mouse tissues revealed that GTPBP1 is expressed in some neurons and smooth muscle cells of various organs as well as macrophages. Immunofluorescence analyses revealed that GTPBP1 is localized exclusively in cytoplasm and shows a diffuse granular network forming a gradient from the nucleus to the periphery of the cells in smooth muscle cell lines and macrophages. To investigate the physiological role of GTPBP1, we used targeted gene disruption in embryonic stem cells to generate GTPBP1-deficient mice. The mutant mice were born at the expected Mendelian frequency, developed normally, and were fertile. No manifest anatomical or behavioral abnormality was observed in the mutant mice. Functions of macrophages, including chemotaxis, phagocytosis, and nitric oxide production, in mutant mice were equivalent to those seen in wild-type mice. No significant difference was observed in the immune response to protein antigen between mutant mice and wild-type mice, suggesting normal function of antigen-presenting cells of the mutant mice. The absence of an eminent phenotype in GTPBP1-deficient mice may be due to functional compensation by GTPBP2, a molecule we recently identified which is similar to GTPBP1 in structure and tissue distribution.

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Section: Methodsmentioning

confidence: 99%

Immunocytochemical Analyses and Targeted Gene Disruption of GTPBP1

Senju

Iyama²,

Kaneko

et al. 2000

Molecular and Cellular Biology

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“…The 32 non-receptor tyrosine kinases fall into 10 subfamilies based on kinase domain sequence. The remaining ®ve sequences are classi®ed as Hubbard and Till, 2000;Hunter, 2000;Robertson et al, 2000;Cole et al, 1999;Fischer, 1999;Schenk and Snaar-Jagalska, 1999;Hubbard et al, 1998;Porter and Vaillancourt, 1998;Johnson et al, 1996;Hanks and Hunter, 1995Non-receptor families Neet and Hunter, 1996ABL Lanier and Gertler, 2000Laneuville, 1995ACK Mott et al, 1999CSK Sondhi and Cole, 1999Klages et al, 1994FAK Avraham et al, 2000Girault et al, 1999;Schlaepfer and Hunter, 1998;Weisberg et al, 1997FES Smithgall et al, 1998Pendergast, 1996FRK Lee, 1998Vasioukhin and Tyner, 1997;Kohmura et al, 1994;Lee et al, 1994 JAK Danial andRothman, 2000;Imada and Leonard, 2000;Aringer et al, 1999;O'Shea, 1998 SRC Abram andCourtneidge, 2000;Schlessinger, 2000;Schwartzberg, 1998;Thomas and Brugge, 1997;Chow andVeillette, 1995 TEC Schaeffer andSchwartzberg, 2000;Yang et al, 2000;Mano, 1999;Rawlings and…”

Section: Searching the Genomementioning

confidence: 99%

The protein tyrosine kinase family of the human genome

2000

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“…Srm, which encodes a protein of 497 amino acids, was cloned from a mouse lung cDNA library and appears to be ubiquitously expressed in tissues (Kohmura et al 1994). It exhibits structural hallmarks of both the Src and Csk family members: it contains an SH3 and an SH2 domain and the conserved autophosphorylation site in the PTK catalytic domain, but lacks an Nterminal myristoylation signal and a C-terminal regulatory Tyr (Fig.…”

Section: Srmmentioning

confidence: 99%

“…The SH3, SH2 and catalytic domains together are 63% similar to mouse c-Src, 64% similar to human FRK, and 55% similar to the Tec family members. Srm-deficient mice have been generated, but disappointingly, these mice have no detectable phenotype (Kohmura et al 1994), and to date there are no clues to a cellular function for Srm.…”

Section: Srmmentioning

confidence: 99%

Vertebrate non‐receptor protein–tyrosine kinase families

1996

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Many non‐receptor protein–tyrosine kinases (PTKs) function as subunits of receptors, either receptors with or without intrinsic PTK catalytic activity of their own. There are currently at least 33 known vertebrate genes that encode non‐receptor PTKs. These can be divided into nine families: Abl, Fes/Fer, Syk/Zap70, Jak, Tec, Fak, Ack, Src, and Csk. Four additional non‐receptor PTKs (Rlk/Txk, Srm, Rak/Frk, and Brk/Sik) do not appear to belong to any of the defined families. Here we review current knowledge of the general roles of non‐receptor PTKs, as well as the characteristic features and functions of each family and its family members.

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A novel nonreceptor tyrosine kinase, Srm: cloning and targeted disruption.

Cited by 48 publications

References 74 publications

Immunocytochemical Analyses and Targeted Gene Disruption of GTPBP1

Immunocytochemical Analyses and Targeted Gene Disruption of GTPBP1

The protein tyrosine kinase family of the human genome

Vertebrate non‐receptor protein–tyrosine kinase families

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