A novel non‐invasive optical method for quantitative visualization of pH dynamics in the rhizosphere of plants

Bloßfeld, Stephan; Gansert, Dirk

doi:10.1111/j.1365-3040.2006.01616.x

Cited by 76 publications

(72 citation statements)

References 52 publications

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“…This technique was initially validated for the measurement of the rhizosphere pH of a plant species (Juncus effusus L.) that grows in water-saturated soils (Blossfeld and Gansert 2007). Proving the suitability of this technique under even moderate soil moisture conditions had still to be done (Hinsinger et al 2009;Luster et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The resulting 2D color contour plots represented the measured pH value of the selected sections of the planar optodes. For further technical details, calibration procedure and data processing, see Blossfeld and Gansert (2007).…”

Section: Plant Species and Cultivationmentioning

confidence: 99%

“…The pH measurements were made using a non-invasive optical technique, described by Blossfeld and Gansert (2007). This technique uses planar pH optodes, based on the measurement of the fluorescence decay time of pH sensitive indicator dyes (Gansert and Blossfeld 2008;Gansert et al 2006;Huber et al 2001;Klimant et al 2001).…”

Section: Plant Species and Cultivationmentioning

confidence: 99%

“…Therefore, to quantify the dynamics of pH at the rhizosphere scale of soil roots grown in trace-metalcontaminated soils, a newly developed method for the non-invasive 2D imaging of pH, based on planar pH optodes (Blossfeld and Gansert 2007) was used and adapted to this specific question. The results of this approach applied to three plant species grown in three unsaturated soils contaminated with Cd, Pb and Zn are presented in this article.…”

Section: Introductionmentioning

confidence: 99%

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Rhizosphere pH dynamics in trace-metal-contaminated soils, monitored with planar pH optodes

Bloßfeld

Perriguey²,

Sterckeman³

et al. 2009

Plant Soil

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The present study presents new insights into pH dynamics in the rhizosphere of alpine pennycress (Noccaea caerulescens (J. Presl & C. Presl) F.K. Mey), maize (Zea mays L.) and ryegrass (Lolium perenne L.), when growing on three soils contaminated by trace metals with initial pH values varying from 5.6 to 7.4. The pH dynamics were recorded, using a recently developed 2D imaging technique based on planar pH optodes. This showed that alpine pennycress and ryegrass alkalinized their rhizosphere by up to 1.7 and 1.5 pH units, respectively, whereas maize acidified its rhizosphere by up to −0.7 pH units. The alkalinization by the roots of alpine pennycress and ryegrass was permanent and not restricted to specific root zones, whereas the acidification along the maize roots was restricted to the elongation zone and thus only temporary. Calculations showed that such pH changes should have noticeable effects on the solubility of the trace metal in the rhizosphere, and therefore on their uptake by the plants. As a result, it is suggested that models for trace metal uptake should include precise knowledge of rhizospheric pH conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Plant Species and Cultivationmentioning

confidence: 99%

Section: Plant Species and Cultivationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rhizosphere pH dynamics in trace-metal-contaminated soils, monitored with planar pH optodes

Bloßfeld

Perriguey²,

Sterckeman³

et al. 2009

Plant Soil

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“…This approach has been extended by combining this technique with videodensiometry (Ruiz and Arvieu 1990). More recently optode foils combined with high resolution optical systems have been used for measurement of pH gradients and CO 2 release (Blossfeld and Gansert 2007;Rudolph-Mohr et al 2015). Bioreporters (i.e., bacteria tagged with fluorescent proteins to report a specific activity) have been used to study the release of AsIII (Kuppardt et al 2010), available nitrate (DeAngelis et al 2005) and the communication of root colonizing rhizobacteria (Gantner et al 2006).…”

Section: Brief Review Of Chemical Mappingmentioning

confidence: 99%

Challenges in imaging and predictive modeling of rhizosphere processes

et al. 2016

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Background Plant-soil interaction is central to human food production and ecosystem function. Thus, it is essential to not only understand, but also to develop predictive mathematical models which can be used to assess how climate and soil management practices will affect these interactions. Scope In this paper we review the current developments in structural and chemical imaging of rhizosphere processes within the context of multiscale mathematical image based modeling. We outline areas that need more research and areas which would benefit from more detailed understanding. Conclusions We conclude that the combination of structural and chemical imaging with modeling is an incredibly powerful tool which is fundamental for understanding how plant roots interact with soil. We emphasize the need for more researchers to be attracted to this area that is so fertile for future discoveries. Finally, model building must go hand in hand with experiments. In particular, there is a real need to integrate rhizosphere structural and chemical imaging with modeling for better understanding of the rhizosphere processes leading to models which explicitly account for pore scale processes.

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Plant‐induced changes in soil processes and properties

Hinsinger

2013

Soil Conditions and Plant Growth

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A novel non‐invasive optical method for quantitative visualization of pH dynamics in the rhizosphere of plants

Cited by 76 publications

References 52 publications

Rhizosphere pH dynamics in trace-metal-contaminated soils, monitored with planar pH optodes

Rhizosphere pH dynamics in trace-metal-contaminated soils, monitored with planar pH optodes

Challenges in imaging and predictive modeling of rhizosphere processes

Plant‐induced changes in soil processes and properties

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