A novel nodule-enhanced gene encoding a putative universal stress protein from Astragalus sinicus

Chou, Ming-Yi; Wei, Xinyuan; Chen, Da-Song; Junchu, Zhou

doi:10.1016/j.jplph.2006.05.009

Cited by 23 publications

(20 citation statements)

References 31 publications

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“…XP_002266746), thus implying it is a novel USP gene. In plants, only a few homologues of the USP family have been isolated (Chou et al, 2007; Maqbool et al, 2009), and their functions remain unclear. The present study demonstrates that SpUSP plays a critical role in abiotic stress, particularly in drought tolerance in tomato, as supported by data from overexpressing lines.…”

Section: Discussionmentioning

confidence: 99%

SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato

Loukehaich¹,

Wang²,

Ouyang

et al. 2012

Journal of Experimental Botany

128

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Universal stress protein (USP) appears to play an active role in the abiotic stress response, but their functions remain largely unknown in plants. A USP gene (SpUSP) was cloned from wild tomato (Solanum pennellii) and functionally characterized in cultivated tomato in the present study. The SpUSP transcript is abundantly accumulated in leaf stomata and its expression varied with the circadian rhythm. SpUSP was remarkably induced by dehydration, salt stress, oxidative stress, and the phytohormone abscisic acid (ABA) etc. This protein was predominantly localized in the nucleus and cell membrane. Overexpressing SpUSP increased drought tolerance of tomato in the seedling and adult stages. Under drought stress, the ABA content significantly increased in the SpUSP-overexpressing plants, which induced stomatal closure and reduced water loss, leading to the enhancement of drought tolerance. Based on the microarray data, a large number of chlorophyll a/b-binding proteins and photosystem-related genes were up-regulated in the SpUSP-overexpressing plants under drought conditions, which possibly enhanced the stomatal sensivitity to ABA and maintained the photosynthetic function. SpUSP overexpression also alleviated the oxidative damage accompanied by oxidative stress-responsive gene activation and osmolyte accumulation. Annexin (SGN-U314161) was found to interacte with SpUSP in the yeast two-hybrid method. This interaction was further confirmed by the bimolecular fluorescence complementation assay. The present study demonstrated that the annexin-interacting SpUSP plays important roles in the drought tolerance of tomato by influencing ABA-induced stomatal movement, increasing photosynthesis, and alleviating oxidative stress.

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Section: Discussionmentioning

confidence: 99%

SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato

Loukehaich¹,

Wang²,

Ouyang

et al. 2012

Journal of Experimental Botany

128

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“…A possible role of usp was discussed in plant regulation processes, during the nodulation of infected roots of Astragalus sinicus (Chou et al 2007). It is also reported to mediate a general process such as cell division and expansion and differentiation of submerged rice plants (Sauter et al 2002).…”

Section: Discussionmentioning

confidence: 99%

GUSP1 and GUSP2, Two Drought-Responsive Genes in Gossypium arboreum Have Homology to Universal Stress Proteins

et al. 2008

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Two closely related genes GUSP1 and GUSP2, within the universal stress protein (USP) family, were identified and cloned from water-stressed leaves of Gossypium arboreum. GUSP1 and GUSP2 genes code for proteins with predicted molecular weights of 18.2 and 19.1 kDa, respectively. Sequence analysis showed that GUSP1 and GUSP2 are highly similar to the bacterial MJ0577-type of adenosine-triphosphate-binding Usp proteins, which have been proposed to function as a molecular switch. Nucleotide sequences of these two genes showed 81% sequence similarity while their encoded proteins share 75% amino acid homology. Both proteins have high percentages of similarity (17% to 61%) to the USPs from a variety of bacteria and plants. Real-time polymerase chain reaction expression analysis revealed a high level of GUSP gene expression in leaves, roots, and stems exclusively in plants following water stress. The highest levels of droughtinducible expression were found in the leaves. A progressive decrease in expression was observed in the stem and roots compared to very low expression in control tissues.

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“…For gene expression analysis, RNA was isolated from infected roots at different dpi (1,3,5,7,9,12,15 and 21 dpi, respectively), infected roots without nodules (27 dpi), nodules (27 dpi), leaves (27 dpi), leafstalks (27 dpi) and uninfected roots (4, 6 and 33 d after sowing, respectively) to conduct semi-quantitative reverse transcription PCR (RT-PCR).…”

Section: Total Rna Isolationmentioning

confidence: 99%

“…Semi-quantitative RT-PCR analysis of mRNA for the two genes under investigation in the total RNA isolated from 4-day-old uninfected roots (4), 6-day-old uninfected roots (harvested just before inoculation)(6), 33-day-old uninfected roots (33), infected rootsat 1 dpi (1), infected roots at 3 dpi (3),, infected roots at 5 dpi (5), infected roots at 7 dpi (7), infected roots at 9 dpi (9), infected roots at 12 dpi (12), infected roots at 15 dpi (15) and infected roots at 21 dpi (21). The 18S rRNA was used as a constitutive control.…”

Section: Figurementioning

confidence: 99%

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Identification of two novel nodule-specific genes from Astragalus sinicus L. by suppressive subtractive hybridization

et al. 2007

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To identify the genes involved in nodule formation and to increase usable molecular probes, a cDNA library of Astragalus sinicus genes specifically expressed in infected roots by Mesorhizobium huakuii 7653R is generated using a PCR-based suppressive subtractive hybridization (SSH) technique with two mRNA populations of infected and uninfected control roots. Two nodule-specific genes, AsIIC259 and AsG2511, are identified from infected roots of A. sinicus. The amino acid sequences deduced from the open reading frames (ORFs) reveal that AsIIC259 and AsG2511 encodes a polypeptide with 134 and 58 amino acids, respectively. A signal peptide sequence is predicted with high probability at the N-termini of the AsIIC259 and AsG2511. The motif searches show that the deduced polypeptide of AsIIC259 contains two N-glycosylation sites, a cAMP-and cGMP-dependent protein kinase phosphorylation site and a casein kinase II phosphorylation site. BLASTP searches reveal that AsIIC259 putative protein displays a low degree of similarity to a unique nodulin from Lupinus luteus nodules. No significant identity is displayed over the predicted polypeptides of AsG2511 with any published sequences. Virtual Northern blot and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analyses indicate that the two genes are expressed exclusively in inoculated roots and that their expression is 2-4 d later than that of the leghaemoglobin (Lb) gene during nodule development.nodule-specific genes, Astragalus sinicus L., nodulins, symbiosis, suppressive subtractive hybridizationThe symbiosis between leguminous plants and rhizobia (including the genera Rhizobium, Bradyrhizobium, Azorhizobium, Sinorhizobium and Mesorhizobium) leads to the formation of a novel root organ, the nodule, hosting nitrogen-fixing rhizobia that feed the host plant with ammonium. This symbiotic association is established by the constant communication between both interacted partners: the plant secrets flavonoid molecules into the rhizosphere, which induce the expression of bacterial lipochito-oligosaccharide signaling molecules, the Nod factors (NFs). Responding to NFs, some host plant nodulin genes are expressed and sometimes cortical cell divisions are initiated to form nodules [1] . The products of plant genes with an induced or enhanced expression during nodule development are termed ''nodulins'' [2] . Depending whether their expressions precede or accompany nitrogen fixation, nodulin genes are classified as early and late nodulins [3] .There are two major classes of nodules formed on legume roots: the indeterminate type and determinate type. Indeterminate nodules usually develop on temperate legumes and display a characteristic zonation. The apical meristem is zone I followed by infection zone II, amyloplast-rich interzones II-III, nitrogen-fixing zone

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A novel nodule-enhanced gene encoding a putative universal stress protein from Astragalus sinicus

Cited by 23 publications

References 31 publications

SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato

SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato

GUSP1 and GUSP2, Two Drought-Responsive Genes in Gossypium arboreum Have Homology to Universal Stress Proteins

Identification of two novel nodule-specific genes from Astragalus sinicus L. by suppressive subtractive hybridization

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