GUSP1 and GUSP2, Two Drought-Responsive Genes in Gossypium arboreum Have Homology to Universal Stress Proteins

Maqbool, Asma; Zahur, Muzna; Husnain, Tayyab; Riazuddin, S.

doi:10.1007/s11105-008-0049-0

Cited by 36 publications

(36 citation statements)

References 32 publications

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“…Expression of several plant USP genes has been shown to be induced under various abiotic stresses, but their precise molecular function is still unknown (Sauter et al, 2002; Maqbool et al, 2009; Loukehaich et al, 2012; Gonzali et al, 2015; Jung et al, 2015; Sinha et al, 2016; Udawat et al, 2016). Two plant USPs, i.e., SpUSP from wild tomato and SbUSP from S. brachiata have been reported to be induced in response to multiple abiotic stresses (Loukehaich et al, 2012; Udawat et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Kerk et al (2003) identified 44 genes encoding USPA domain containing proteins in Arabidopsis and performed their phylogenetic analysis which revealed that they have evolved from 1MJH like ancestor. Since then, several USP proteins have been reported to play important roles in stress tolerance in various plants, such as Arabidopsis , rice, cotton, tomato, pigeonpea, and Salicornia brachiata (Sauter et al, 2002; Lenman et al, 2008; Merkouropoulos et al, 2008; Maqbool et al, 2009; Loukehaich et al, 2012; Udawat et al, 2014, 2016; Gonzali et al, 2015; Jung et al, 2015; Sinha et al, 2016). However, the precise function of most of the plant USPs has not been deciphered, so far.…”

Section: Introductionmentioning

confidence: 99%

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The Promoter of AtUSP Is Co-regulated by Phytohormones and Abiotic Stresses in Arabidopsis thaliana

et al. 2016

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Universal stress proteins (USPs) are known to be expressed in response to various abiotic stresses in a wide variety of organisms, such as bacteria, archaebacteria, protists, algae, fungi, plants, and animals. However, in plants, biological function of most of the USPs still remains obscure. In the present study, Arabidopsis USP gene (AtUSP) showed induction in response to abscisic acid (ABA) and various abiotic stresses viz. heat, dehydration, salt, osmotic, and cold stresses. Additionally, in silico analysis of AtUSP promoter identified several cis-elements responsive to phytohormones and abiotic stresses such as ABRE, ERE, DRE, and HSE, etc. To functionally validate the AtUSP promoter, the 1115 bp region of promoter was characterized under phytohormone and abiotic stress treatments. Deletion analysis of promoter was carried out by cloning the full length promoter (D0) and its three 5′ deletion derivatives, D1 (964 bp), D2 (660 bp), and D3 (503 bp) upstream of the β-glucuronidase (GUS) reporter gene, which were then stably transformed in Arabidopsis plants. The AtUSP promoter (D0) showed minimal activity under non-stress conditions which was enhanced in response to phytohormone treatments (ABA and ACC) and abiotic stresses such as dehydration, heat, cold, salt, and osmotic stresses. The seedlings harboring D1 and D2 deletion fragments showed constitutive GUS expression even under control condition with increased activity almost under all the treatments. However, D3 seedlings exhibited complete loss of activity under control condition with induction under ACC treatment, dehydration, heat, oxidative, salt, and osmotic stresses. Thus, present study clearly showed that AtUSP promoter is highly inducible by phytohormones and multiple abiotic stresses and it can be exploited as stress inducible promoter to generate multi-stress tolerant crops with minimal effects on their other important traits.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Promoter of AtUSP Is Co-regulated by Phytohormones and Abiotic Stresses in Arabidopsis thaliana

et al. 2016

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“…USPs have been shown to be present among a diverse group of organisms such as bacteria, protists, fungi and plants (Table 1) [48,49,50,51,52,53,54,55,56,57,58,59], assisting them to tolerate different environmental and adverse conditions such as high salinity, high temperatures, drought and toxic chemicals [49]. Originally, universal stress protein A was first discovered in Escherichia coli but lately, genes encoding proteins with the USP domain (Pfam Identifier: PF00582) have recently been shown to be present in the S. mansoni , S. japonicum and S. haematobium species [17,49,60].…”

Section: Universal Stress Proteins Mode Of Action and Factors Thamentioning

confidence: 99%

“…Several duplicates of USPs may exist in certain organisms such as the six Usps present in Escherichia coli (UspA, UspC ( yecG ), UspD ( yiiT ), UspE ( ydaA ), UspF ( ynaF ) and UspG ( ybdQ )) and they may also be encoded by different gene families [52,58]. There are 8 known S. mansoni USP genes: (Smp_001000, Smp_001010, Smp_031300, Smp_043120, Smp_076400, Smp_097930, Smp_136870 and Smp_136890) and all have shown conserved sites for Aspartate, Leucine, Glycine, Histidine and Proline which seem to be common functional sites for regulating these USPs [60].…”

Section: Universal Stress Proteins Mode Of Action and Factors Thamentioning

confidence: 99%

Universal Stress Proteins as New Targets for Environmental and Therapeutic Interventions of Schistosomiasis

Masamba

Adenowo

Oyinloye

et al. 2016

IJERPH

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In spite of various control measures and eradication methods that have been in progress, schistosomiasis still prevails as one of the most prevalent debilitating parasitic diseases, typically affecting the poor and the underprivileged that are predominantly concentrated in sub-Saharan Africa. The parasitic schistosome blood fluke responsible for causing the disease completes its complex developmental cycle in two hosts: humans and freshwater snails, where they physically undergo gross modifications to endure the different conditions associated with each host. Just like any other organism, the worm possesses mechanisms that help them respond to environmental insults. It has been hypothesized that a special class of proteins known as Universal Stress Proteins (USPs) are up-regulated during sudden environmental changes, thus assisting the worm to tolerate the unfavourable conditions associated with its developmental cycle. The position of praziquantel as the drug of choice against all schistosome infections has been deemed vulnerable due to mounting concerns over drug pressure and so the need for alternative treatment is now a matter of urgency. Therefore, this review seeks to explore the associations and possible roles of USPs in schistosomiasis as well as the functioning of these proteins in the schistosomulae stage in order to develop new therapeutic interventions against this disease.

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“…Drought stress is one of the main abiotic stressors that limit plant growth and production (Yang et al 2010;Wang et al 2011;Maqbool et al 2009). Water deficiency is a threat to agriculture because of the inability to control water availability without the use of irrigation strategies (Prabu et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Three Sorbitol Transporter Genes in Micropropagated Apple Plants Grown under Drought Stress

Lei

Zhao

et al. 2011

Plant Mol Biol Rep

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Sorbitol, a major end-product of photosynthesis in many species of the Rosaceae family, accumulates in response to abiotic stressors. However, the relationship that arises between the expression of sorbitol transporters and sorbitol accumulation under abiotic stress remains unclear. In this study, micropropagated 'Fuji' apple plants (Malus domestica Borkh. 'Fuji') were exposed to two varying degrees of osmotic stress and compared relative to an unstressed control. The osmotic stress was generated by adding PEG 6000 into full-strength Hoagland solution and adjusted the osmotic potential to either −0.75 MPa (mild drought stress [MIS]) or −1.5 MPa (severe drought stress [SES]). Analysis of sorbitol levels via high performance liquid chromatography (HPLC) showed that the sorbitol concentration was elevated in roots, phloem tissues and leaves in both the MIS and SES treatments compared to controls for the entire duration of the experiment. Three cDNA sequences, encoding sorbitol transporters (MdSOT3, MdSOT4 and MdSOT5), were isolated from leaves. Realtime quantitative PCR (RT-qPCR) data suggests that the expression levels of MdSOT3 and MdSOT5 were higher under MIS and SES in roots, phloem tissues and leaves compared to unstressed controls. The average mRNA levels of MdSOT4 in phloem tissues declined under both drought treatments (with the exception being at 2 h of SES). In roots and leaves under SES, mRNA production was increased. These results indicate that the up-regulation of MdSOT3 and MdSOT5 expression is consistent with the accumulation of sorbitol under conditions of osmotic stress in apple plants. They enhanced drought tolerance in vegetative tissues. Increased MdSOT4 mRNA enhanced drought tolerance under SES.

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GUSP1 and GUSP2, Two Drought-Responsive Genes in Gossypium arboreum Have Homology to Universal Stress Proteins

Cited by 36 publications

References 32 publications

The Promoter of AtUSP Is Co-regulated by Phytohormones and Abiotic Stresses in Arabidopsis thaliana

The Promoter of AtUSP Is Co-regulated by Phytohormones and Abiotic Stresses in Arabidopsis thaliana

Universal Stress Proteins as New Targets for Environmental and Therapeutic Interventions of Schistosomiasis

Characterization of Three Sorbitol Transporter Genes in Micropropagated Apple Plants Grown under Drought Stress

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