A Novel Model of Obesity-Related Diabetes: Introgression of the Leprfa Allele of the Zucker Fatty Rat into Nonobese Spontaneously Diabetic Torii (SDT) Rats

Masuyama, Taku; Katsuda, Yoshiaki; Shinohara, Masami

doi:10.1538/expanim.54.13

Cited by 75 publications

(101 citation statements)

References 22 publications

(42 reference statements)

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“…Body weight, serum parameters, except for the leptin level, and renal-related parameters except for the urine angiotensinogen level were determined in previous studies [4,8]. The results of the present study are shown in Table 1.…”

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confidence: 68%

“…Moreover, metabolic syndrome is defined worldwide as a lifestyle disease, and is described as a clustering of multiple metabolic abnormalities and cardiovascular risk factors, including hypertension, obesity, hyperlipidemia, and insulin resistance [3]. From these and other perspectives, it is an important to examine the relation between glycolipid abnormalities and blood pressure.The Spontaneously Diabetic Torii-Lepr fa (SDT-fa/fa) rat is a new model of obese type 2 diabetes, and was established by introducing the fa gene of the Zuckerfatty rat into the SDT rat genome [8]. SDT-fa/fa rats exhibit hyperphagia and obesity immediately after weaning, and diabetes and its complications are found at a younger age in SDT-fa/fa rats than in SDT-+/+ rats [4,9,10,12].…”

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confidence: 99%

“…rat is a new model of obese type 2 diabetes, and was established by introducing the fa gene of the Zuckerfatty rat into the SDT rat genome [8]. SDT-fa/fa rats exhibit hyperphagia and obesity immediately after weaning, and diabetes and its complications are found at a younger age in SDT-fa/fa rats than in SDT-+/+ rats [4,9,10,12].…”

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Evaluation of Blood Pressure in Spontaneously Diabetic Torii-Leprfa Rats

et al. 2010

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Abstract:The Spontaneously Diabetic Torii-Lepr fa (SDT-fa/fa) rat, a new model of obese type 2 diabetes, shows obesity, hyperglycemia, and hyperlipidemia from 6 weeks of age. Diabetic complications such as nephropathy and cataract are observed with aging; however, blood pressure change with age has not previously been examined. In this study, blood pressure was periodically measured and the change was investigated. Blood pressure in male SDT-fa/fa rats was elevated at 8, 16, and 24 weeks of age, whereas the heart rate was not changed. In addition to hyperglycemia, hyperlipidemia, and proteinuria, hyperleptinemia and increased urine angiotensinogen were observed in SDT-fa/fa rats. Blood pressure and heart rate in the male original SDT (SDT-+/+) rat did not significantly change. In conclusion, the SDT-fa/fa rat is a promising model, showing significant hypertension with diabetes mellitus. Key words: blood pressure, diabetes, SDT-fa/fa rat (Received 8 February 2010 / Accepted 21 March 2010 Address corresponding: T. Ohta, Japan Tobacco Inc., Central Pharmaceutical Research Institute, 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan Cardiovascular diseases are major causes of death in persons with diabetes, and many factors, including hypertension, contribute to this high prevalence of cardiovascular disease. Hypertension, a common comorbid condition in dysmetabolic syndrome, often occurs in patients with type 2 diabetes, and vigorous control of blood pressure decreases the rate of cardiovascular events in such patients. Control of high blood pressure represents the most important intervention for such patients, limiting cardiovascular events more effectively than tight glycemic control [1,2,15]. Moreover, metabolic syndrome is defined worldwide as a lifestyle disease, and is described as a clustering of multiple metabolic abnormalities and cardiovascular risk factors, including hypertension, obesity, hyperlipidemia, and insulin resistance [3]. From these and other perspectives, it is an important to examine the relation between glycolipid abnormalities and blood pressure.The Spontaneously Diabetic Torii-Lepr fa (SDT-fa/fa) rat is a new model of obese type 2 diabetes, and was established by introducing the fa gene of the Zuckerfatty rat into the SDT rat genome [8]. SDT-fa/fa rats exhibit hyperphagia and obesity immediately after weaning, and diabetes and its complications are found at a younger age in SDT-fa/fa rats than in SDT-+/+ rats [4,9,10,12]. However, blood pressure in SDT-fa/fa rats has not yet been examined. In the present study, we periodically investigated the blood pressure of male -Note-

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Evaluation of Blood Pressure in Spontaneously Diabetic Torii-Leprfa Rats

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“…The Spontaneously Diabetic Torii (SDT) rat is also known to be a non-obese T2DM rat that displays impaired insulin secretion [17]. Introduction of the fa mutation caused the SDT rat to develop obesity and hyperinsulinemia [16]. Evidently, a deficiency of leptin receptor, which causes abnormal hyperphagia, can induce some components of metabolic syndrome, namely obesity, hyperlipidemia, hyperinsulinemia and insulin resistance.…”

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confidence: 99%

Characterization of STZ-Induced Type 2 Diabetes in Zucker Fatty Rats

et al. 2008

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“…Consequently, several experimental models have been utilized. 102,[131][132][133][134] The diet-induced obesity animal model -in which obesity is achieved via modulation of the diet -appears to provide the best mimic of the human obese condition. Although no clear pattern is evident, in most cases, morbid obesity is caused by an increase of the relative amount of fat in the diet.…”

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Organic consequences of ileal transposition in rats with diet-induced obesity

et al. 2011

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INTRODUCTION:The clinical management of metabolic syndrome -especially diabetes mellitus type 2 -is notoriously complex due to the progressive nature of this disease. At present, there is a need for a surgical procedure that is effective for the treatment of diabetes mellitus type 2, even in non-obese individuals. The isolated ileal transposition theory could lead to an effective alternative therapy. This intervention has not yet been performed in humans, and there are no reports of its use in an experimental model of diet-induced metabolic syndrome. OBJECTIVES:The objective of this study is to evaluate the physiological effects of ileal transposition in rats with diet-induced metabolic syndrome. The effects of this procedure on glucose and lipid metabolism will be assessed. METHODS:Forty 12-week-old male rats (albino Rattus norvegicus, Wistar, 2BAW, heterogeneous) will be divided into four groups of 10 animals each: the ileal transposition group (TG) comprising animals on a hypercaloric-hyperlipidic diet; the sham group (SG) containing animals that receive the same diet and undergo the sham surgery; control group 1 (CG1), which will receive a hypercaloric-hyperlipidic diet and will not undergo surgery; and control group 2 (CG2), which will consume standard feed and will not undergo surgery. The surgeries will be performed in 20-week-old animals.Blood samples for laboratory testing will be collected from 12-week-old animals on the day of surgery and after eight postoperative weeks, following determination of the weights of the animals and the administration of anesthesia. The levels of serum glucose, insulin, triglycerides, total cholesterol and fractions, glucagon-like peptide-1, C-peptide and glycated hemoglobin will be assessed in all of the animals. The insulin tolerance test will be performed using PRISMA software, and insulin resistance will be calculated by the HOMA-IR indirect test. On specific days, two 20-week-old rats will be separated and randomly distributed in TG and SG. These animals will be followed until the eighth postoperative week. Subsequently, they will be euthanized, and the retroperitoneal and periepididymal fat deposits will be collected and weighed using a precision scale. In addition, the pancreas, liver and intestinal segments will be sent for pathological and immunohistochemical studies.

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A Novel Model of Obesity-Related Diabetes: Introgression of the Leprfa Allele of the Zucker Fatty Rat into Nonobese Spontaneously Diabetic Torii (SDT) Rats

Abstract: An fa allele of the leptin receptor gene (Lepr fa ) of the Zucker fatty rat was introduced into the genome of the Spontaneously

Cited by 75 publications

References 22 publications

Evaluation of Blood Pressure in Spontaneously Diabetic Torii-Leprfa Rats

Evaluation of Blood Pressure in Spontaneously Diabetic Torii-Leprfa Rats

Characterization of STZ-Induced Type 2 Diabetes in Zucker Fatty Rats

Organic consequences of ileal transposition in rats with diet-induced obesity

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