A novel model of humin

Lichtfouse, Éric

doi:10.1051/analusis:1999270385

Cited by 17 publications

(19 citation statements)

References 13 publications

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“…The literature analysis shows that mumie is a geopolymer, which can coiling and folding, especially with high molecular weight species (proteins, lipids) and producing hydrophobic domains that can serve as sequestration sites for small molecules (for example, steroid nature) [Lichtfouse, 1999]. Some mumie polymers with polysaccharide nature can bind cellular receptors and activate the processes of cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

Osteoblastic differentiation of mesenchymal stem cells by mumie extract

Jung

Schepetkin

Woo

et al. 2002

Drug Development Research

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Mumie, a plant humus matter from rocks, is known as anabolic and a stimulator of bone regeneration in the Russian and Indian systems of health and medicine. The water-soluble fraction of mumie from Uzbekistan was characterized using 1 H NMR and infrared spectroscopic methods. The mumie extract has been investigated for its effect on osteoblastic differentiation in cell culture assays of human and murine mesenchymal stem cells. The calcium deposition and expression of alkaline phosphatase, osteocalcin, core binding factor 1 (Cbfa1), and ERK have been studied. During the 14-day assay period, human bone marrow mesenchymal stem cells (hMSCs) and human fetal osteoblasts cultured with mumie (3-5 mg/ml) underwent a dramatic change in cellular morphology, which was accompanied by a significant increase in alkaline phosphatase activity, calcium deposition, and osteocalcin expression. The expression of core binding factor 1 and ERK were enhanced in hMSCs and murine pluripotent mesenchymal precursor cell line C2C12. Dose-dependent decrease in TRAP-positive multinucleated cell formation from macrophage-like cells RAW 264.7 was observed with increasing concentration of mumie in the presence of RANKL (40 ng/ml) and PD98059 (10 mM), a specific inhibitor of ERK activity. The data suggest that mumie is a potent stimulator of osteoblastic differentiation of mesenchymal stem cells and inhibitor of osteoclastogenesis, hence it maybe of clinical benefit in the treatment for osteoporosis in human. Drug Dev. Res. 57: 122-133, 2002.

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Section: Discussionmentioning

confidence: 99%

Osteoblastic differentiation of mesenchymal stem cells by mumie extract

Jung

Schepetkin

Woo

et al. 2002

Drug Development Research

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“…Certain recalcitrant plant and microbial biopolymers are thought to be incorporated into stable SOM with only minor modification by microorganisms (Hatcher and Spiker, 1988;Lichtfouse, 1999;Waksman, 1932). Early investigators held the view, popularized by the Waks-man (1932) lignin-protein theory, that humus was derived primarily from the incomplete utilization of lignin by microorganisms.…”

Section: Selective Preservationmentioning

confidence: 99%

Biogeochemistry of Decomposition and Detrital Processing

Sanderman¹,

Amundson²

2014

Treatise on Geochemistry

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“…Selectively preserved, straight‐chain, microbial biopolymers and apolar substances physically encapsulated by weak forces and by covalent bonds (Lichtfouse, 1999).…”

Section: Introductionmentioning

confidence: 99%

Sequential exhaustive extraction of a Mollisol soil, and characterizations of humic components, including humin, by solid and solution state NMR

Song¹,

Novotny²,

Simpson³

et al. 2008

European J Soil Science

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A comprehensive sequential extraction procedure was applied to isolate soil organic components using aqueous solvents at different pH values, base plus urea (base-urea), and finally dimethylsulfoxide (DMSO) plus concentrated H 2 SO 4 (DMSO-acid) for the humin-enriched clay separates. The extracts from baseurea and DMSO-acid would be regarded as 'humin' in the classical definitions. The fractions isolated from aqueous base, base-urea and DMSO-acid were characterized by solid and solution state NMR spectroscopy. The base-urea solvent system isolated ca. 10% (by mass) additional humic substances. The combined base-urea and DMSO-acid solvents isolated ca. 93% of total organic carbon from the humin-enriched fine clay fraction (<2 mm). Characterization of the humic fractions by solid-state NMR spectroscopy showed that oxidized char materials were concentrated in humic acids isolated at pH 7, and in the base-urea extract. Lignin-derived materials were in considerable abundance in the humic acids isolated at pH 12.6. Only very small amounts of char-derived structures were contained in the fulvic acids and fulvic acids-like material isolated from the base-urea solvent. After extraction with baseurea, the 0.5 M NaOH extract from the humin-enriched clay was predominantly composed of aliphatic hydrocarbon groups, and with lesser amounts of aromatic carbon (probably including some char material), and carbohydrates and peptides. From the combination of solid and solution-state NMR spectroscopy, it is clear that the major components of humin materials, from the DMSO-acid solvent, after the exhaustive extraction sequence, were composed of microbial and plant derived components, mainly long-chain aliphatic species (including fatty acids/ester, waxes, lipids and cuticular material), carbohydrate, peptides/proteins, lignin derivatives, lipoprotein and peptidoglycan (major structural components in bacteria cell walls). Black carbon or char materials were enriched in humic acids isolated at pH 7 and humic acids-like material isolated in the base-urea medium, indicating that urea can liberate charderived material hydrogen bonded or trapped within the humin matrix.

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A novel model of humin

Cited by 17 publications

References 13 publications

Osteoblastic differentiation of mesenchymal stem cells by mumie extract

Osteoblastic differentiation of mesenchymal stem cells by mumie extract

Biogeochemistry of Decomposition and Detrital Processing

Sequential exhaustive extraction of a Mollisol soil, and characterizations of humic components, including humin, by solid and solution state NMR

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