A novel method to prepare concentrated conidial biomass formulation of<i>Trichoderma harzianum</i>for seed application

Singh, Purnima; Nautiyal, Chandra Shekhar

doi:10.1111/j.1365-2672.2012.05426.x

Cited by 37 publications

(20 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The seeds were surface sterilized in 70% ethanol for 10 min and 1% NaOCl for 2 min and then washed four times with sterilized distilled water. Dry powder of the Trichoderma isolates was mixed with sterilized 1.5% xanthan gum gel following the procedure described previously [ 54 , 55 ]. We used a Trichoderma spore powder for seed coating that was easy to handle and enhanced the practical applicability at the farmer level.…”

Section: Methodsmentioning

confidence: 99%

Trichoderma Enhances Net Photosynthesis, Water Use Efficiency, and Growth of Wheat (Triticum aestivum L.) under Salt Stress

et al. 2020

View full text Add to dashboard Cite

Soil salinity is one of the most important abiotic stresses limiting plant growth and productivity. The breeding of salt-tolerant wheat cultivars has substantially relieved the adverse effects of salt stress. Complementing these cultivars with growth-promoting microbes has the potential to stimulate and further enhance their salt tolerance. In this study, two fungal isolates, Th4 and Th6, and one bacterial isolate, C7, were isolated. The phylogenetic analyses suggested that these isolates were closely related to Trichoderma yunnanense, Trichoderma afroharzianum, and Bacillus licheniformis, respectively. These isolates produced indole-3-acetic acid (IAA) under salt stress (200 mM). The abilities of these isolates to enhance salt tolerance were investigated by seed coatings on salt-sensitive and salt-tolerant wheat cultivars. Salt stress (S), cultivar (C), and microbial treatment (M) significantly affected water use efficiency. The interaction effect of M x S significantly correlated with all photosynthetic parameters investigated. Treatments with Trichoderma isolates enhanced net photosynthesis, water use efficiency and biomass production. Principal component analysis revealed that the influences of microbial isolates on the photosynthetic parameters of the different wheat cultivars differed substantially. This study illustrated that Trichoderma isolates enhance the growth of wheat under salt stress and demonstrated the potential of using these isolates as plant biostimulants.

show abstract

Section: Methodsmentioning

confidence: 99%

Trichoderma Enhances Net Photosynthesis, Water Use Efficiency, and Growth of Wheat (Triticum aestivum L.) under Salt Stress

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In agriculture, Trichoderma species are robust biological control agents and are often added to soils to increase crop yields and control soil-borne pathogen worldwide. It has been estimated that in India alone, more than 250 Trichoderma -based formulations are sold commercially [ 9 – 11 ]. Moreover, since Trichoderma species possess innate resistance to many chemicals used in agriculture such as fungicides, they are readily integrated into pest management practices [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Volatile organic compounds emitted by Trichoderma species mediate plant growth

Lee

Yap

Behringer

et al. 2016

Fungal Biol Biotechnol

238

159

View full text Add to dashboard Cite

Background: Many Trichoderma species are applied as biofungicides and biofertilizers to agricultural soils to enhance crop growth. These filamentous fungi have the ability to reduce plant diseases and promote plant growth and productivity through overlapping modes of action including induced systemic resistance, antibiosis, enhanced nutrient efficiency, and myco-parasitism. Trichoderma species are prolific producers of many small metabolites with antifungal, antibacterial, and anticancer properties. Volatile metabolites of Trichoderma also have the ability to induce resistance to plant pathogens leading to improved plant health. In this study, Arabidopsis plants were exposed to mixtures of volatile organic compounds (VOCs) emitted by growing cultures of Trichoderma from 20 strains, representing 11 different Trichoderma species.Results: We identified nine Trichoderma strains that produced plant growth promoting VOCs. Exposure to mixtures of VOCs emitted by these strains increased plant biomass (37.1-41.6 %) and chlorophyll content (82.5-89.3 %). Trichoderma volatile-mediated changes in plant growth were strain-and species-specific. VOCs emitted by T. pseudokoningii (CBS 130756) were associated with the greatest Arabidopsis growth promotion. One strain, T. atroviride (CBS 01-209), in our screen decreased growth (50.5 %) and chlorophyll production (13.1 %). Similarly, tomatoes exposed to VOCs from T. viride (BBA 70239) showed a significant increase in plant biomass (>99 %), larger plant size, and significant development of lateral roots. We also observed that the tomato plant growths were dependent on the duration of the volatile exposure. A GC-MS analysis of VOCs from Trichoderma strains identified more than 141 unique compounds including several unknown sesquiterpenes, diterpenes, and tetraterpenes. Conclusions:Plants grown in the presence of fungal VOCs emitted by different species and strains of Trichoderma exhibited a range of effects. This study demonstrates that the blend of volatiles produced by actively growing fungi and volatile exposure time in plant development both influence the outcome of volatile-mediated interactions. Only some of our growth promoting strains produced microbial VOCs known to enhance plant growth. Compounds such as 6-pentyl-2H-pyran-2-one were not common to all promoting strains. We found that biostimulatory strains tended to have a larger number of complex terpenes which may explain the variation in growth induced by different Trichoderma strains.

show abstract

“…As temperature seemed to have very adverse effect on the viability of the isolates to produce non-volatile inhibitors and inhibition zone has been found to observe great eat at optimum temperatures which results in increase bio mass production as confirmed by Tronsmo et al, 1978;Singh et al, 2014. In additions, selected carbon and nitrogen sources found to be most suitable substrates significantly enhanced growth and population of Trichodermas pecies (Kredicset al, 2003). Among the selected carbon and nitrogen sources found to be most effective for growth and multiplication of Trichoderma may be due to Fungi use a large number of organic compounds as a carbon sources and about half of the dry weight of the fungus cells consists of carbon, which gives an indication of the important role of carbon compounds within the cell (Singh et al, 2012;Rai et al, 2016). CONCLUSION Isolation and morphological characterization are the important tools that leads to further steps for Trichoderma biomass production at different nutritional and environmental parameters.…”

Section: Discussionmentioning

confidence: 99%

“…Biocontrol antagonists play an important role in the management of plant diseases (Hajieghrari et al, 2008;Alwathnani and Perveen, 2012;Li et al, 2013). Generally, commercial production of Trichoderma largely depends upon bulk conidia production that reproduces asexually (Verma et al, 2007;Singh and Nautiyal, 2012). Mass production of Trichoderma conidia relies on physical and environmental parameters that promote conidiation for commercial production of Trichoderma species (Harman et al, 2010;Singh et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Effect of Physiological Parameters on Mass Production of Trichoderma Species

Ghazanfar

Raza

2018

Pak. J. Phytopathol.

View full text Add to dashboard Cite

A B S T R A C TMycophagous fungi show antagonistic potential against plant pathogenic fungi and suggested as a promising biocontrol agent that influences by nutritional and environmental parameters. The present research was conducted with the aim to determine optimal environmental and nutritional parameters range for biomass production of Trichoderma. The two species of Trichoderma isolated from rhizosphere of citrus, wheat and tomato of different localities of district Sargodha, Punjab, Pakistan and later on effect of temperature, carbon, nitrogen and pH were investigated on three strain of T. harzianum HM, HK, HC and one strain of T. asperellum TH under in vitro conditions. All strains produced statistically sufficient mass production at all temperatures but 25 °C as well as 30 °C were found ideal for mass production of Trichoderma. A substantial difference in mass production of Trichoderma isolates was recorded at different pH levels includes 4.0, 5.0, 6.0, 7.0 and 8.0. The pH range from 5.0 to 7.0 found to be optimum for all species of Trichoderma. All fungal species of Tricoderma produced extensive mass production on growth media supplemented with carbon and nitrogen sources such as mannose, glactose, sucrose, lactose and sodium nitrite (NaNO3), potassium nitrate (KNO3), ammonium nitrate (NH4NO3) as well as ammonium nitrite (NH4NO3) respectively. The optimal mass production was recorded on carbon supplemented growth medium as compared to nitrogen supplemented growth medium. Therefore, T. harzianum HK produced maximum biomass production among all isolates of Trichoderma.

show abstract

A novel method to prepare concentrated conidial biomass formulation ofTrichoderma harzianumfor seed application

Cited by 37 publications

References 29 publications

Trichoderma Enhances Net Photosynthesis, Water Use Efficiency, and Growth of Wheat (Triticum aestivum L.) under Salt Stress

Trichoderma Enhances Net Photosynthesis, Water Use Efficiency, and Growth of Wheat (Triticum aestivum L.) under Salt Stress

Volatile organic compounds emitted by Trichoderma species mediate plant growth

Effect of Physiological Parameters on Mass Production of Trichoderma Species

Contact Info

Product

Resources

About