A novel method of preserving cardiac grafts using a hydrogen-rich water bath

Noda, Kentaro; Shigemura, Norihisa; Tanaka, Yugo; Kawamura, Tomohiro; Lim, Sang Hyun; Kokubo, Kenichi; Billiar, Timothy R.; Bermúdez, C.; Kobayashi, Hirosuke; Nakao, Atsunori

doi:10.1016/j.healun.2012.11.004

Cited by 48 publications

(42 citation statements)

References 38 publications

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“…In this study, we reveal that H 2 blocks ROS accumulation induced by β-adrenoceptor stimulation both in vitro and in vivo. The inhibitory effects of H 2 on ROS also have been reported in various animal models, such as heart ischemia/reperfusion injury (Zhang et al, 2011; Noda et al, 2013; Shinbo et al, 2013), brain injury (Ohsawa et al, 2007; Liu et al, 2011; Wang et al, 2012), renal injury (Li et al, 2016), chemotherapy-induced ovarian injury (Meng et al, 2015), metabolic syndrome (Song et al, 2013), etc. NADPH oxidase and mitochondria have been proposed as primary sites of ROS generation (Dai et al, 2011a).…”

Section: Discussionmentioning

confidence: 89%

Hydrogen (H2) Inhibits Isoproterenol-Induced Cardiac Hypertrophy via Antioxidative Pathways

Zhang

Long

et al. 2016

Front. Pharmacol.

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Background and Purpose: Hydrogen (H2) has been shown to have a strong antioxidant effect on preventing oxidative stress-related diseases. The goal of the present study is to determine the pharmacodynamics of H2 in a model of isoproterenol (ISO)-induced cardiac hypertrophy.Methods: Mice (C57BL/6J; 8–10 weeks of age) were randomly assigned to four groups: Control group (n = 10), ISO group (n = 12), ISO plus H2 group (n = 12), and H2 group (n = 12). Mice received H2 (1 ml/100g/day, intraperitoneal injection) for 7 days before ISO (0.5 mg/100g/day, subcutaneous injection) infusion, and then received ISO with or without H2 for another 7 days. Then, cardiac function was evaluated by echocardiography. Cardiac hypertrophy was reflected by heart weight/body weight, gross morphology of hearts, and heart sections stained with hematoxylin and eosin, and relative atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) mRNA levels. Cardiac reactive oxygen species (ROS), 3-nitrotyrosine and p67 (phox) levels were analyzed by dihydroethidium staining, immunohistochemistry and Western blotting, respectively. For in vitro study, H9c2 cardiomyocytes were pretreated with H2-rich medium for 30 min, and then treated with ISO (10 μM) for the indicated time. The medium and ISO were re-changed every 24 h. Cardiomyocyte surface areas, relative ANP and BNP mRNA levels, the expression of 3-nitrotyrosine, and the dissipation of mitochondrial membrane potential (MMP) were examined. Moreover, the expression of extracellular signal-regulated kinase1/2 (ERK1/2), p-ERK1/2, p38, p-p38, c-Jun NH2-terminal kinase (JNK), and p-JNK were measured by Western blotting both in vivo and in vitro.Results: Intraperitoneal injection of H2 prevented cardiac hypertrophy and improved cardiac function in ISO-infused mice. H2-rich medium blocked ISO-mediated cardiomyocytes hypertrophy in vitro. H2 blocked the excessive expression of NADPH oxidase and the accumulation of ROS, attenuated the decrease of MMP, and inhibited ROS-sensitive ERK1/2, p38, and JNK signaling pathways.Conclusion: H2 inhibits ISO-induced cardiac/cardiomyocytes hypertrophy both in vivo and in vitro, and improves the impaired left ventricular function. H2 exerts its protective effects partially through blocking ROS-sensitive ERK1/2, p38, and JNK signaling pathways.

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Section: Discussionmentioning

confidence: 89%

Hydrogen (H2) Inhibits Isoproterenol-Induced Cardiac Hypertrophy via Antioxidative Pathways

Zhang

Long

et al. 2016

Front. Pharmacol.

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“…Similarly, Noda et al 34 documented that a novel hydrogen-supplemented preservation solution efficiently improved myocardial injury due to cold I/R in a rat heterotopic transplantation model. In this study, the hydrogen-rich preservation solution led to decreased levels of IL-6, IL-1β, TNF-α, ICAM-1, iNOS, and CCL2, which are all markers of inflammation.…”

Section: Introductionmentioning

confidence: 92%

Hydrogen, a potential safeguard for graft-versus-host disease and graft ischemia-reperfusion injury?

Yuan¹,

Shen²

2016

Clinics

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Post-transplant complications such as graft-versus-host disease and graft ischemia-reperfusion injury are crucial challenges in transplantation. Hydrogen can act as a potential antioxidant, playing a preventive role against post-transplant complications in animal models of multiple organ transplantation. Herein, the authors review the current literature regarding the effects of hydrogen on graft ischemia-reperfusion injury and graft-versus-host disease. Existing data on the effects of hydrogen on ischemia-reperfusion injury related to organ transplantation are specifically reviewed and coupled with further suggestions for future work. The reviewed studies showed that hydrogen (inhaled or dissolved in saline) improved the outcomes of organ transplantation by decreasing oxidative stress and inflammation at both the transplanted organ and the systemic levels. In conclusion, a substantial body of experimental evidence suggests that hydrogen can significantly alleviate transplantation-related ischemia-reperfusion injury and have a therapeutic effect on graft-versus-host disease, mainly via inhibition of inflammatory cytokine secretion and reduction of oxidative stress through several underlying mechanisms. Further animal experiments and preliminary human clinical trials will lay the foundation for hydrogen use as a drug in the clinic.

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“…Delivery of H 2 by inhalation or injection with H 2 [12, 13], injection with H 2 -rich saline [14, 15], drinking H 2 -rich water [16, 17], taking an H 2 -rich bath [18], and increasing the production of intestinal H 2 by bacteria [19], has been shown to protect against cardiovascular and metabolic diseases, such as atherosclerosis, glucose and lipid metabolism disorder, myocardial ischemia/reperfusion (I/R) injury, myocardial transplantation injury, or cardiovascular hypertrophy. All of these will be discussed below.…”

Section: Hydrogen Gas-background To It Usementioning

confidence: 99%

“…However, the cardiac transplant procedure obligates cold preservation and warm reperfusion of cardiac grafts and results in a certain degree of I/R injury in all grafts [18, 53]. The injury occurring during preservation or reperfusion can affect cardiac function after heart transplantation.…”

Section: The Effects Of H2 In Heart Diseasesmentioning

confidence: 99%

“…Reducing injury is important for preserving cardiac function. Importantly, H 2 -rich Histidine–Tryptophan–Ketoglutarate (HTK), H 2 inhalation, drinking H 2 -rich water or H 2 -rich water bath have the abilities to inhibit oxidative stress, suppress immune and inflammatory responses, improve mitochondria function and energy metabolism, enhance graft survival, and attenuate cardiac injury during preservation or reperfusion in heart transplantation [18, 53-55]. …”

Section: The Effects Of H2 In Heart Diseasesmentioning

confidence: 99%

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Hydrogen Therapy in Cardiovascular and Metabolic Diseases: from Bench to Bedside

Zhang

Tan

et al. 2018

Cell Physiol Biochem

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Hydrogen (H₂) is colorless, odorless, and the lightest of gas molecules. Studies in the past ten years have indicated that H₂ is extremely important in regulating the homeostasis of the cardiovascular system and metabolic activity. Delivery of H₂ by various strategies improves cardiometabolic diseases, including atherosclerosis, vascular injury, ischemic or hypertrophic ventricular remodeling, intermittent hypoxia- or heart transplantation-induced heart injury, obesity and diabetes in animal models or in clinical trials. The purpose of this review is to summarize the physical and chemical properties of H₂, and then, the functions of H₂ with an emphasis on the therapeutic potential and molecular mechanisms involved in the diseases above. We hope this review will provide the future outlook of H₂-based therapies for cardiometabolic disease.

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A novel method of preserving cardiac grafts using a hydrogen-rich water bath

Cited by 48 publications

References 38 publications

Hydrogen (H2) Inhibits Isoproterenol-Induced Cardiac Hypertrophy via Antioxidative Pathways

Hydrogen (H2) Inhibits Isoproterenol-Induced Cardiac Hypertrophy via Antioxidative Pathways

Hydrogen, a potential safeguard for graft-versus-host disease and graft ischemia-reperfusion injury?

Hydrogen Therapy in Cardiovascular and Metabolic Diseases: from Bench to Bedside

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