A Novel Method of Core Aspirate Cytology Compared to Fine-Needle Aspiration for Diagnosing Canine Osteosarcoma

Neihaus, Steven A.; Locke, Jennifer E.; Barger, Anne M.; Borst, Luke B.; Goring, Robert L.

doi:10.5326/jaaha-ms-5676

Cited by 19 publications

(13 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…20,21 Similar studies in veterinary medicine reports accuracies between 97 and 69% in differentiating benign and malignant lesions. 11,15,22,23 Several elements partially limit the interpretation and comparison of the results with these studies. In some of those, the histologic diagnosis being compared with cytological diagnosis was indifferently obtained from surgical/post-mortem samples or from small incisional biopsies.…”

Section: Discussionmentioning

confidence: 94%

“…The main limitation of these methods is that reactive osteoblasts will stain positive as well, so criteria of malignancy must be assessed. 15,[25][26][27] Overall, the concordance of cytology and histology with the final diagnosis was not completely satisfactory.…”

Section: Discussionmentioning

confidence: 99%

“…Representative examples of cytological specimens are provided in Figure 4. The mean number of slides per case was 5 (range, [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17]. Cellularity was poor in 16 cases (30.2%) and moderate to high in the remaining 37 cases (69.8%).…”

Section: Fine-needle Aspiration Cytology Diagnosesmentioning

confidence: 99%

“…12 However, it also has several limitations, including the inability to evaluate tissue architecture; this might prompt a generic diagnosis of malignancy without further classification of tumor type. [13][14][15] In addition, clinicians can be concerned about the difficulty in performing an adequate cytological sampling from a bone lesion, because of the challenge of penetrating the bone cortex.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Comparative Assessment of the Accuracy of Cytological and Histologic Biopsies in the Diagnosis of Canine Bone Lesions

Sabattini

Renzi

Buracco

et al. 2017

Veterinary Internal Medicne

View full text Add to dashboard Cite

BackgroundOsteosarcoma (OSA) should be differentiated from other less frequent primary bone neoplasms, metastatic disease, and tumor‐like lesions, as treatment and prognosis can vary accordingly. Hence, a preoperative histologic diagnosis is generally preferred. This requires collection of multiple biopsies under general anesthesia, with possible complications, including pathological fractures. Fine‐needle aspiration cytology would allow an earlier diagnosis with a significant reduction of discomfort and morbidity.Hypothesis/ObjectivesThe aim of this study was to compare the accuracy of cytological and histologic biopsies in the diagnosis of canine osteodestructive lesions.AnimalsSixty‐eight dogs with bone lesions.MethodsRetrospective study. Accuracy was assessed by comparing the former diagnosis with the final histologic diagnosis on surgical or post‐mortem samples or, in the case of non‐neoplastic lesions, with follow‐up information.ResultsThe study included 50 primary malignant bone tumors (40 OSAs, 5 chondrosarcomas, 2 fibrosarcomas, and 3 poorly differentiated sarcomas), 6 carcinoma metastases, and 12 non‐neoplastic lesions. Accuracy was 83% for cytology (sensitivity, 83.3%; specificity, 80%) and 82.1% for histology (sensitivity, 72.2%; specificity, 100%). Tumor type was correctly identified cytologically and histologically in 50 and 55.5% of cases, respectively.Conclusions and Clinical ImportanceThe accuracy of cytology was similar to histology, even in the determination of tumor type. In no case was a benign lesion diagnosed as malignant on cytology. This is the most important error to prevent, as treatment for malignant bone tumors includes aggressive surgery. Being a reliable diagnostic method, cytology should be further considered to aid decisions in the preoperative setting of canine bone lesions.

show abstract

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Fine-needle Aspiration Cytology Diagnosesmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Comparative Assessment of the Accuracy of Cytological and Histologic Biopsies in the Diagnosis of Canine Bone Lesions

Sabattini

Renzi

Buracco

et al. 2017

Veterinary Internal Medicne

View full text Add to dashboard Cite

show abstract

“…The sensitivity of cytology for diagnosing histologically-confirmed osteosarcomas as sarcomas was high, ranging from 79.2% to 95.0%, and its specificity was 100% in three out of four studies. 4,[8][9][10] The sensitivity of cytology in identifying the exact histotype (i.e. providing a specific diagnosis of osteosarcoma rather than a generic diagnosis of sarcoma) was investigated only by Loukopoulos et al (2005) and Sabattini et al (2017), and it was found to be lower (25.0% and 42.9%, respectively).…”

Section: Appraisal Application and Reflectionmentioning

confidence: 99%

Diagnostic accuracy of cytology for canine osteosarcoma compared to histopathology

Oikonomidis

Tsouloufi

2021

View full text Add to dashboard Cite

PICO question What is the sensitivity and specificity of cytology as a test for canine osteosarcoma when compared to histopathology as a gold standard? Clinical bottom line Category of research question Diagnosis The number and type of study designs reviewed Overall, four diagnostic validity studies (two prospective and two retrospective) were critically appraised Strength of evidence Weak to moderate Outcomes reported There is evidence of moderate strength to support that cytology is highly sensitive and specific for diagnosing histologically confirmed osteosarcomas as mesenchymal malignant neoplasms (cytological diagnosis of sarcoma). Evidence of weak strength suggests that the sensitivity and specificity of cytology for identifying the exact histotype (cytological diagnosis of osteosarcoma) are low and high, respectively. Finally, there is currently evidence of weak strength indicating that the sensitivity and specificity of cytology are comparable to that of preoperative histopathology after incisional biopsy for the diagnosis of canine osteosarcoma Conclusion Based on the available evidence, the diagnostic accuracy of cytology in diagnosing histologically confirmed osteosarcomas as sarcomas is high, whereas a confident conclusion cannot be drawn regarding the diagnostic accuracy of cytology for the identification of the exact histotype (cytological diagnosis of osteosarcoma). There is currently scant evidence suggesting that cytology has comparable diagnostic accuracy to preoperative histopathology (i.e. after incisional biopsy) for the diagnosis of canine osteosarcomas, however, more studies are warranted to confirm these results How to apply this evidence in practice The application of evidence into practice should take into account multiple factors, not limited to: individual clinical expertise, patient’s circumstances and owners’ values, country, location or clinic where you work, the individual case in front of you, the availability of therapies and resources. Knowledge Summaries are a resource to help reinforce or inform decision making. They do not override the responsibility or judgement of the practitioner to do what is best for the animal in their care.

show abstract

Musculoskeletal Tumors

Liptak¹,

Dernell²,

Farese³

et al. 2022

Veterinary Surgical Oncology

View full text Add to dashboard Cite

A Novel Method of Core Aspirate Cytology Compared to Fine-Needle Aspiration for Diagnosing Canine Osteosarcoma

Cited by 19 publications

References 20 publications

Comparative Assessment of the Accuracy of Cytological and Histologic Biopsies in the Diagnosis of Canine Bone Lesions

Comparative Assessment of the Accuracy of Cytological and Histologic Biopsies in the Diagnosis of Canine Bone Lesions

Diagnostic accuracy of cytology for canine osteosarcoma compared to histopathology

Musculoskeletal Tumors

Contact Info

Product

Resources

About