A novel method for inward fluid displacement in centrifugal microdevices for highly integrated nucleic acid processing with long-term reagent storage

Dignan, Leah M.; Karas, Scott M; Mighell, Isabella K.; Treene, William R.; Landers, James P.; Woolf, M. Shane

doi:10.1016/j.aca.2022.340063

Cited by 6 publications

(4 citation statements)

References 48 publications

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“…Two separate instances where this has been effectively demonstrated on a platform include Dignan et al (Fig. 10C) 247 and Aeinehvand et al (Fig. 10D).…”

Section: Structural Applicationsmentioning

confidence: 97%

“…[243][244][245] This can be particularly useful during heating steps which cause fluidic expansion and risk liquid escape. 22,246,247 They may also act as physical barriers capable of avoiding analyst exposure to potentially toxic compounds or contamination of nucleic acid amplification tests (NAATs). 248 Intriguingly, they may also be efficiently leveraged to help generate pneumatic pressure on-disc to drive fluid displacement.…”

Section: Structural Applicationsmentioning

confidence: 99%

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Integrated membranes within centrifugal microfluidic devices: a review

O'Connell

Landers

2023

Lab Chip

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“…Two separate instances where this has been effectively demonstrated on a platform include Dignan et al (Fig. 10C) 247 and Aeinehvand et al (Fig. 10D).…”

Section: Structural Applicationsmentioning

confidence: 97%

Section: Structural Applicationsmentioning

confidence: 99%

Integrated membranes within centrifugal microfluidic devices: a review

O'Connell

Landers

2023

Lab Chip

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“…The on-disc sample handling is implemented by a centrifugal force and the various valves are involved in sample/solution transfer between different sample preparation processes [95,96]. These disc devices are most frequently used for protein [38,97], genes [65,98], bacteria [64,99], and cell [100,101] analysis containing multiple processes. Some of these discs are employed as user-friendly sample preparation equipment for the off-line detection, such as LC-MS/MS [38,97].…”

Section: Lab-on-a-chip Sample Preparation Techniquesmentioning

confidence: 99%

Recent progress of microfluidic sample preparation techniques

Xia

2023

J of Separation Science

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Sample preparation turns out to be one of the important procedures in complex sample analysis by affecting the accuracy, selectivity, and sensitivity of analytical results. However, the majority of the conventional sample preparation techniques still suffer from time‐consuming and labor‐intensive operations. These shortcomings can be addressed by reforming the sample preparation process in a microfluidic manner. Inheriting the advantages of rapid, high efficiency, low consumption, and easy integration, microfluidic sample preparation techniques receive increasing attention, including microfluidic phases separation, microfluidic field‐assisted extraction, microfluidic membrane separation, and microfluidic chemical conversion. This review overviews the progress of microfluidic sample preparation techniques in the last 3 years based on more than 100 references, we highlight the implementation of typical sample preparation methods in the formats of microfluidics. Furthermore, the challenges and outlooks of the application of microfluidic sample preparation techniques are discussed.

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“…In a notable exception, using immiscible liquid check valves with pumping structures has permitted efficient pumping at more sedate acceleration/deceleration profiles [24]. Chemical pumping, where gas is released from a chemical reaction and displaces a liquid inwards, has also been demonstrated to have a good effect [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Dissolvable Film-Controlled Buoyancy Pumping and Aliquoting on a Lab-On-A-Disc

et al. 2023

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Lab-on-a-Disc (LoaD) has great potential for applications in decentralised bioanalytical testing where speed and robustness are critical. Here, a disc-shaped microfluidic chip is rotated to pump liquid radially outwards; thus, all microfluidic structures must be fitted into the available radial length. To overcome this limitation, several centripetal pumping technologies have been developed. In this work, we combine buoyancy pumping, enabled by displacing aqueous samples and reagents centripetally inwards by a dense liquid (fluorocarbon FC-40), with dissolvable film (DF) to automate a multi-step assay. The DF dissolves in the presence of water but is not in contact with the FC-40. Therefore, the FC-40 can be stored behind the DF membranes and is autonomously released by contact with the arriving aqueous sample. Using this technology, tasks such as blood centrifugation can be located on the disc periphery where ‘disc real estate’ is less valuable and centrifugal forces are higher. To demonstrate this, we use the combination of the buoyancy-driven centripetal pumping with DF barriers to implement a fully automated multi-parameter diagnostic assay on the LoaD platform. The implemented steps include plasma extraction from a structure, automatically triggered metering/aliquoting, and the management of five onboard stored liquid reagents. Critically, we also demonstrate highly accurate aliquoting of reagents using centripetal pumping. We also provide a mathematical model to describe the pumping mechanism and apply lumped-element modelling and Monte Carlo simulation to estimate errors in the aliquoting volumes caused by manufacturing deviations.

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A novel method for inward fluid displacement in centrifugal microdevices for highly integrated nucleic acid processing with long-term reagent storage

Cited by 6 publications

References 48 publications

Integrated membranes within centrifugal microfluidic devices: a review

Integrated membranes within centrifugal microfluidic devices: a review

Recent progress of microfluidic sample preparation techniques

Dissolvable Film-Controlled Buoyancy Pumping and Aliquoting on a Lab-On-A-Disc

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