A novel method for flow injection analysis of total antioxidant capacity using enzymatically produced ABTS+ and biamperometric detector containing interdigitated electrode

Milardović, Stjepan; Kereković, Irena; Derrico, Romano; Rumenjak, Vlatko

doi:10.1016/j.talanta.2006.03.042

Cited by 35 publications

(30 citation statements)

References 19 publications

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“…The applied interdigitated electrode (IDE) detector accomplished a good sensitivity of 0.3 nA/ µM Trolox and offered linear range between 20 to 500µM Trolox. Real samples like juices, tea and wine were analysed [99].…”

Section: The Biamperometric Methodmentioning

confidence: 99%

Methods for Total Antioxidant Activity Determination: A Review

Pisoschi¹,

Negulescu²

2012

Biochem & Anal Biochem

325

302

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Section: The Biamperometric Methodmentioning

confidence: 99%

Methods for Total Antioxidant Activity Determination: A Review

Pisoschi¹,

Negulescu²

2012

Biochem & Anal Biochem

325

302

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“…The supernatant of the cells was lysed using a radioimmunoprecipitation assay (RIPA) buffer (Nanjing KeyGen Biotech Co., Ltd., Nanjing, China) in order to measure the TAC using the azino-diethyl-benzthiazoline sulfate (ABTS) method (24). Total antioxidant capacity detection kit was purchased from Nanjing KeyGen Biotech Co., Ltd. Incubation of ABTS with H 2 O 2 and a peroxidase (metmyoglobin) produces a blue-green radical cation, ABTS + .…”

Section: Methodsmentioning

confidence: 99%

α- and β-Naphthoflavone synergistically attenuate H2O2-induced neuron SH-SY5Y cell damage

Zhu

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Previous studies have demonstrated an association between neurological diseases and oxidative stress (OS).Naphthoflavone is a synthetic derivative of naturally occurring flavonoids that serves an important role in the treatment and prevention of OS-related diseases. The current study was designed to apply α-and β-Naphthoflavone individually and in combination to counteract the detrimental effects of OS on neurons in vitro. Neuronal SH-SY5Y cells were subjected to 20 µM H 2 O 2 , followed by exposure to 20 µM α-Naphthoflavone and/or 10 µM β-Naphthoflavone. Results indicated that α-and β-Naphthoflavone effectively antagonized the apoptosis-promoting effect of H 2 O 2 on neuronal SH-SY5Y cells, and that β-Naphthoflavone significantly (P<0.05) reversed H 2 O 2 -inhibited cell viability. Notably, co-treatment of α-and β-Naphthoflavone reversed the H 2 O 2 -induced apoptosis rate elevation and cell viability reduction. Further analysis demonstrated that H 2 O 2 inhibited the activities of antioxidant enzymes including catalase, superoxide dismutase and glutathione peroxidase, but this was reversed by the co-treatment with α-and β-Naphthoflavone and selectively enhanced by the treatment with α-or β-Naphthoflavone. H 2 O 2 -stimulated p38 mitogen-activated protein kinase activation was repressed following treatment with α-and/or β-Naphthoflavone, along with a decreased expression of the apoptosis-related factors and inhibited caspase-3 activation. In conclusion, co-treatment with α-and β-Naphthoflavone minimized H 2 O 2 -led neuron damage compared with treatment with α-or β-Naphthoflavone, suggesting a synergetic effect between α-and β-Naphthoflavone. This indicates that utilizing α-and β-Naphthoflavone together in the clinical setting may provide a novel therapeutic for neurological disease.

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“…The confirmed high selectivity of the biamperometric method allows the quantification of redox active analytes present in biological samples (urine, blood), and, also, of the total antioxidant capacity of foodstuffs and beverages [113][114][115][116]. Nevertheless, biamperometry requires very rigorous control of experimental conditions; the method's performances with respect to selectivity relying upon the specificity of the reaction between the oxidized or reduced form of the redox couple and the analyte [113][114][115][116].…”

Section: Biamperometrymentioning

confidence: 99%

“…A peroxidase-containing flow system generated the cation radical, aiming at the assessment of antioxidant activity in juices, tea and wine at interdigitated microelectrodes [115], or the cation radical could be obtained by using glucose oxidase and peroxidase, for the analysis of wine and spirits with enhanced sensitivity, 0.165 nA/μM Trolox [116].…”

Section: Biamperometrymentioning

confidence: 99%

Electrochemical Methods for Total Antioxidant Capacity and its Main Contributors Determination: A review

2015

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Abstract:Backround: The present review focuses on electrochemical methods for antioxidant capacity and its main contributors assessment. The main reactive oxygen species, responsible for low density lipoprotein oxidation, and their reactivity are reminded. The role of antioxidants in counteracting the factors leading to oxidative stress-related degenerative diseases occurence, is then discussed. Antioxidants can scavenge free radicals, can chelate pro-oxidative metal ions, or quench singlet oxygen. When endogenous factors (uric acid, bilirubin, albumin, metallothioneins, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-

show abstract

A novel method for flow injection analysis of total antioxidant capacity using enzymatically produced ABTS+ and biamperometric detector containing interdigitated electrode

Cited by 35 publications

References 19 publications

Methods for Total Antioxidant Activity Determination: A Review

Methods for Total Antioxidant Activity Determination: A Review

α- and β-Naphthoflavone synergistically attenuate H2O2-induced neuron SH-SY5Y cell damage

Electrochemical Methods for Total Antioxidant Capacity and its Main Contributors Determination: A review

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