A novel method for fast and statistically verified morphological characterization of filamentous fungi

Posch, Andreas E.; Spadiut, Oliver; Herwig, Christoph

doi:10.1016/j.fgb.2012.05.003

Cited by 41 publications

(47 citation statements)

References 33 publications

(38 reference statements)

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confidence: 99%

“…They can grow as freely dispersed mycelia or as densely compacted biomass granules, depending on the strain characteristics as well as the environmental conditions [4 5]. Posch et al [4] classified the morphology of filamentous fungi into two major discriminative classes: disperse growth and pellet growth. In submerged cultures, the morphology of filamentous microorganisms usually varies between the pelleted and dispersed forms, which depends on the cultivation conditions [567].…”

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Optimization of Fungal Enzyme Production byTrichoderma harzianumKUC1716 through Surfactant-Induced Morphological Changes

Lee

Heo

et al. 2017

Mycobiology

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The morphological optimization of Trichoderma harzianum was carried out using several surfactants to achieve increased cellulase production. Addition of the surfactants to the culture medium successfully modified the fungal morphology from an aggregated form to a dispersed form. Optimization of the fungal morphology increased cellulase activity up to 177%. The morphologically optimized conditions enhanced the accessibility of the fungus to substrates and thus promoted cellulase production.

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confidence: 99%

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confidence: 99%

Optimization of Fungal Enzyme Production byTrichoderma harzianumKUC1716 through Surfactant-Induced Morphological Changes

Lee

Heo

et al. 2017

Mycobiology

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“…The critical diameter of pellets at which point oxygen diffusion limitation occur is partially depending on cultivation condition and pellets structure (Driouch et al, ). In previous publication, the critical diameters are in the range of 100–800 µm for different filamentous fungi (Nielsen, ; Posch et al, ; Tang and Zhong, ). Obviously, the diameter of the largest pellet (around 1,200 µm) in this study was beyond the critical diameter, since dense outer layer and hallow core were observed.…”

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confidence: 92%

Improved lipid accumulation by morphology engineering of oleaginous fungus Mortierella isabellina

Gao

Zeng

et al. 2014

Biotech & Bioengineering

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Oleaginous fungi capable of accumulating a considerable amount of lipids are promising sources for lipid-based biofuel production. The specific productivities of filamentous fungi in submerged fermentation are often correlated with morphological forms. However, the relationship between morphological development and lipid accumulation is not known. In this study, distinct morphological forms of oleaginous fungus Mortierella isabellina including pellets of different sizes, free dispersed mycelia, and broken hyphal fragments were developed by additions of different concentrations of magnesium silicate microparticles. Different morphological forms led to different levels of lipid accumulation as well as different spatial patterns of lipid distribution within pellets/mycelial aggregates. Significant higher lipid content (0.75 g lipid/g cell biomass) and lipid yield (0.18 g lipid/g glucose consumed) were achieved in free dispersed mycelia than in pellets. Moreover, extracellular metabolite analysis showed that production of undesirable by-product malate was repressed in free dispersed mycelium form. Unveiling the desired morphological form of M. isabellina for lipid accumulation provided insights into molecular mechanism of lipid biosynthesis linked with morphological development, as well as design and optimization of bioprocess to produce lipid-based biofuels.

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“…This form is also favored because it significantly improves culture rheology, which results in better mass and oxygen transfer into the biomass and lower energy consumption for aeration and agitation. Numerous studies have been carried out to control fungal morphology in pellet form [6][7][8]. However, most early studies mainly focused on environmental factors, such as medium composition, inoculum, pH, medium shear, additives (polymers, surfactants, and chelators), culture temperature, and medium viscosity.…”

Section: Introductionmentioning

confidence: 99%

Effects of Calcium on the Morphology of Rhizopus oryzae and L-lactic Acid Production

Jiang

et al. 2015

Lecture Notes in Electrical Engineering

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The effects of exogenous calcium on fungal pellet morphology during preculture and L-lactic acid production were studied. The results showed that addition of exogenous calcium could induce pellet formation. The diameter of the pellet increased with increasing concentration of exogenous calcium, including CaCl 2 and CaCO 3 . The smaller pellet precultured with low concentration of soluble calcium (CaCl 2 ) was beneficial for L-lactic acid production because the pellet was dense and the large inner part of the pellet was inactive. By contrast, the larger pellet precultured with high concentration of insoluble calcium (CaCO 3 ), except 8.0 g/L CaCO 3 , was beneficial for L-lactic acid production. Supported by the CaCO 3 powder, the entire biomass layer was fully active, and the highest L-lactic acid productivities of 1.22 g/L h and 58.6 g/L L-lactic acid were reached using the 1.5 mm pellet.

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A novel method for fast and statistically verified morphological characterization of filamentous fungi

Cited by 41 publications

References 33 publications

Optimization of Fungal Enzyme Production byTrichoderma harzianumKUC1716 through Surfactant-Induced Morphological Changes

Optimization of Fungal Enzyme Production byTrichoderma harzianumKUC1716 through Surfactant-Induced Morphological Changes

Improved lipid accumulation by morphology engineering of oleaginous fungus Mortierella isabellina

Effects of Calcium on the Morphology of Rhizopus oryzae and L-lactic Acid Production

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