Optimization of Fungal Enzyme Production by<i>Trichoderma harzianum</i>KUC1716 through Surfactant-Induced Morphological Changes

Lee, Hanbyu; Lee, Young Min; Heo, Young Mok; Hong, Jeong Hee; Jang, Seokyoon; Ahn, Byoung Jun; Lee, Sung Suk; Kim, Jae Jin

doi:10.5941/myco.2017.45.1.48

Cited by 15 publications

(4 citation statements)

References 19 publications

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“…Notably, our results are in agreement with previous studies reporting that the pellet form of filamentous fungi is conducive to producing a higher product production [ 35 , 36 ]. Moreover, we confirmed that the addition of 1.5% TEOA into the seed culture medium is conducive to efficient organic acid fermentation by R. oryzae .…”

Section: Resultssupporting

confidence: 93%

Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine

Zhang

et al. 2021

Biotechnol Biofuels

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Rhizopus oryzae (R. oryzae) can effectively produce organic acids, and its pellet formation in seed cultures has been shown to significantly enhance subsequent fermentation processes. Despite advances in strain development, simple and effective methods for inducing pellet morphology and a basic understanding of the mechanisms controlling this process could facilitate substantial increases in efficiency and product output. Here, we report that 1.5% triethanolamine (TEOA) in seed culture medium can activate the growth of R. oryzae spores in compact and uniform pellets which is optimal for fermentation conditions. Analysis of fermentation kinetics showed that the production of fumaric and L-malic acid increases 293% and 177%, respectively. Transcriptomic analysis revealed that exposure of R. oryzae to 1.5% TEOA during the seed culture activated the phosphatidylinositol and mitogen-activated protein kinase signaling pathways. Theses pathways subsequently stimulated the downstream carbohydrate-active synthases and hydrolases that required for cell wall component synthesis and reconstruction. Our results thus provide insight into the regulatory pathways controlling pellet morphology germane to the viability of seed cultures, and provide valuable reference data for subsequent optimization of organic acid fermentation by R. oryzae.

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Section: Resultssupporting

confidence: 93%

Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine

Zhang

et al. 2021

Biotechnol Biofuels

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“…The present findings showed that the three exogenous additives evaluated in this study had different effects on mycelium morphology of ABSC, which may affect biomass and EPS production. Similarly, Lee et al [39] evaluated the addition of surfactant to Trichoderma harzianum fermentation culture medium and observed that Fig. 6 The number of differentially expressed genes (DEGs; log 2 fold change) of ABSC grown in the absence or presence of LRM anthocyanins Fig.…”

Section: Discussionmentioning

confidence: 98%

Anthocyanin extract from Lycium ruthenicum enhanced production of biomass and polysaccharides during submerged fermentation of Agaricus bitorquis (Quél.) Sacc. Chaidam

Chen

et al. 2021

Bioprocess Biosyst Eng

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Agaricus bitorquis (Quél.) Sacc. Chaidam (ABSC) is a wild edible fungus uniquely found in the Tibet Plateau. ABSC is rich in polysaccharides that are considered biologically active. This study aimed to determine the feasibility of enhancing exopolysaccharide (EPS) production by ABSC in shake flask culture by supplementing the fermentation medium with anthocyanin extract. Different concentrations of Lycium ruthenicum Murr. (LRM) anthocyanin crude extract were tested on ABSC fermentation. The activity of phosphoglucose isomerase (PGI), phosphoglucose mutase (PGM), and phosphomannose isomerase (PMI), enzymes presumably involved in EPS synthesis by ABSC, was determined. ABSC transcriptomic profile in response to the presence of anthocyanins during fermentation was also investigated. LRM anthocyanin crude extract (0.06 mg/mL) was most effective in increasing EPS content and mycelial biomass (by 208.10% and 105.30%, respectively, P < 0.01). The activity of PGI, PGM, and PMI was increased in a medium where LRM anthocyanin extract and its main components (proanthocyanidins and petunia anthocyanin) were added. RNA-Seq analysis showed that 349 genes of ABSC were differentially expressed during fermentation in the medium containing anthocyanin extract of LRM; 93 genes were up-regulated and 256 genes down-regulated. From gene ontology enrichment analysis, differentially expressed genes were mostly assigned to carbohydrate metabolism and signal transduction categories. Collectively, LRM anthocyanins extract positively affected EPS production and mycelial biomass during ABSC fermentation. Our study provides a novel strategy for improving EPS production and mycelial growth during ABSC liquid submerged fermentation.

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“…This can be explained by the fact that there was prolonged contact with fungal mycelia and enzymatic process with time and as such more reaction with the increased volume of enzymes in the medium (Echeveria, et al, 2020). The fungus T. harzianum, has more enzymes that can effectively degrade crude oil than A. flavus (Lee, et al, 2017). T. harzianum also colonized the contaminated substrates faster than A. flavus as shown by mycelial growth inhibition (Table 4).…”

Section: Plectosphaerellaceae -+mentioning

confidence: 99%

Bioremediation Potential of Selected Rhizosphere Fungi of Tridax Procumbens Linn. and Chromolaena odorata (L.) R.M. King & H. Rob

Adongbede¹,

Olomu²

2022

Nig. J. Biotechnol

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Co-metabolism between plants and rhizosphere microbes is the mainstay of rhizoremediation of contaminated soils. The aim of the current study was to isolate and screen selected rhizosphere micro-fungi of two Asteraceae (Tridax procumbens and Chromolaena odorata) collected from the wild in University of Lagos, Akoka, Lagos State for bioremediation potential. Rhizosphere fungi were isolated, identified and evaluated for crude oil myco-remediation. The inhibitory effect of different concentrations of crude oil on mycelial growth of the most abundant fungi was determined with poisoned plate assay method. The most abundant and recurring fungi around C. odorata and T. procumbens were determined using the serial dilution and plating methods. Analysis of the rhizosphere soil of T. procumbens and C. odorata showed they were sandy loam type. C. odorata soil had higher moisture, organic carbon, and acid content than T. procumbens. Aspergillus flavus and Trichoderma harzianum were the most abundant and recurring fungi in C. odorata and T. procumbens, respectively. Thirty-nine micro-fungi belonging to twenty genera were isolated from the test plants’ rhizosphere. A. flavus and T. harzianum tolerated 2.50 to 10.00% crude oil contamination assessed with their mycelial growth inhibition reducing with time. A. flavus and T. harzianum caused a 68.45% and 86.71% reduction of crude oil contamination respectively, in a time dependent manner. The filamentous fungi ~A. flavus and T. harzianum can potentially be used to simultaneously ameliorate crude oil contaminated soils in conjunction with C. odorata and T. procumbens, respectively in the innovative technology termed rhizoremediation.

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Optimization of Fungal Enzyme Production byTrichoderma harzianumKUC1716 through Surfactant-Induced Morphological Changes

Cited by 15 publications

References 19 publications

Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine

Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine

Anthocyanin extract from Lycium ruthenicum enhanced production of biomass and polysaccharides during submerged fermentation of Agaricus bitorquis (Quél.) Sacc. Chaidam

Bioremediation Potential of Selected Rhizosphere Fungi of Tridax Procumbens Linn. and Chromolaena odorata (L.) R.M. King & H. Rob

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