A novel method for determining the parameters of microbial kinetics

Abstract

SummaryIt is conventional to describe the relationship between the specific rate of microbial growth and the concentration of the inhibitory substrate in terms of the Andrews-Edwards equation. A novel method for establishing the constants of this equation is presented. The equation is transformed to a polynomial and the empirical data are approximated by a quadratic polynomial. The results obtained for the biodegradation of phenol in a mixed culture (activated sludge) are discussed.

Steady state phenol degradation in a draft‐tube, gas‐liquid‐solid fluidized‐bed bioreactor

“…The growth kinetic parameters derived were: H , , ,~~ = 0.365 h-', K, = 10.948 x kg/m3, and Ki = 1 13.00 x kg/m3. These numerical values are very close to those reported in literature for mixed cultures growing on phe- no1 (D'Adamo et al, 1984;Szetela and Winnicki, 1981;Pawlowsky and Howell, 1973). The double-substrate limiting kinetics used in the biofilm model assumes that phenol and oxygen are limiting substrates.…”

Effect of consecutive pulsing of an inhibitory substrate on biodegradation kinetics

“…Figures 1 and 3 indicate that as phenol pulse frequency increased there was a corresponding decrease in phenol removal rates. In parallel, with increasing phenol pulse frequency, there was an initial delay in batch growth, as depicted in Analytical integration of eq 4 termined values of gm, Ks, and are tabulated in Tables II and III for the first and second set of consecutive experiments, respectively. The values of KB did not change appreciably (KB values ranged 3.6-9.9 mg/L in both sets of experiments with an average value of 8.03 ± 0.85 in the first set and 7.01 ± 0.54 in the second set) with increasing phenol pulse frequency in both studies, and they are about the same for both studies.…”

“…They fitted the biodegradation data to the Haldane equation and to its modified forms classified by Edwards (3) and reported average values for pm, Kg, and for the Haldane equation as 0.26 h-1, 25.4 mg/L, and 173.0 mg/L, respectively. Szetela and Winnicki (4) reported values of the Haldane equation constants, pm, Kg, and as 0.33 hr1, 19.2 mg/L, and 229.0 mg/L, respectively, for phenol.…”

Relevance of the Luong model to describe the biodegradation of phenol by mixed culture in a batch reactor

“…Phenol biodegradation by bacteria is generally inhibited by phenol itself. The Haldane and Monod equations have frequently been used to describe this degradation in pure or mixed cultures (Allsop et al 1993;Beyenal et al 1997;D'Adamo et al 1984;Hill and Robinson 1975;Lallai et al 1988;Pawlowsky and Howell 1973;Sokol 1987;Sokol and Howell 1981;Szetela and Winnicki 1981;Tang and Fan 1987;Yang and Humphrey 1975;).…”