A Novel Metal Adsorbent Composed of a Hexa-histidine Tag and a Carbohydrate-binding Module on Cellulose

Abstract

We developed a novel metal adsorbent composed of bio-based materials, cellulose and a protein. The approach involved the immobilization of hexa-histidine tag (His6), which shows an affinity for intermediate acid (metal ion) in Hard and Soft Acids and Bases (HSAB) theory, on cellulose by fusing with carbohydrate-binding module (CBM). The results show that CBM-His6-bound cellulose has adsorption selectivity reflecting the original properties of His6. Additionally, we prepared three configurations of CBM-His6 proteins, which were subsequently immobilized on a filter paper for Ni 2+ ion adsorption. Of these configurations, we found the protein containing two His6 tags at each terminus (N-and C-) of CBM exhibited the highest metal adsorption ability. Furthermore, XPS analysis confirmed the binding of Ni 2+ ions on the cellulose.

Biological Route to Fabricate Silica on Cellulose Using Immobilized Silicatein Fused with a Carbohydrate-Binding Module

“…The CBM used for this study belongs to Family 3, which shows the affinity on noncrystalline cellulose and crystalline cellulose . The same CBM fused with metal-binding proteins and peptides has been successfully immobilized on the cellulose material in recent studies. , Figure shows the time course of adsorption of proteins on cellulose. ProS2-Sil-CBM showed a 98% adsorption on cellulose within 1.5 h, confirming that it can rapidly bind to cellulose.…”

Combination of cellulose nanofiber and artificial fusion protein for biocementation

“…The synthetic gene of calcite-binding peptide (CaBP: DVFSSFNLKHMR) derived from the phage-display system (Sarikaya et al, 2003) was purchased from GenScript (Tokyo, Japan). The carbohydrate-binding module, CBM3 (hereafter named as CBM), from Clostridium thermocellum (Accession, Q06851) (Hong et al, 2007;Mwandira et al, 2020;Togo et al, 2020) optimized for the expression in E. coli was purchased from Eurofins Genomics (Tokyo, Japan). Tryptone and yeast extract were purchased from BD Biosciences Advanced Bioprocessing (Miami, FL, United States).…”

“…As shown in Figure 3B, over 90% of the fusion protein was adsorbed to CNF, while adsorption to Avicel was quite lower. CBM was well-known to be bound to cellulosic materials (Hong et al, 2007;Mwandira et al, Frontiers in Built Environment frontiersin.org 2020; Togo et al, 2020), mostly through hydrophobic interaction (Chang et al, 2018). CBM3 is classified as type A, which has a surface-binding property, while type B and type C have glycan chain-binding and small sugar-binding properties (Boraston et al, 2004).…”