Fabrication of silica on chitin in ambient conditions using silicatein fused with a chitin-binding domain

Interfacial biosilica coating of chitosan gel using fusion silicatein to fabricate robust hybrid material for biomolecular applications

“…ChBD and Sil were responsible for the binding fusion silicatein on chitin or chitosan, and silica polymerisation, respectively. 27 The silica polymerisation activity of InaKC-ChBD-Sil was 9.5 μM min −1 . 27…”

“…ChBD and Sil were responsible for the binding fusion silicatein on chitin or chitosan, and silica polymerisation, respectively. 27 The silica polymerisation activity of InaKC-ChBD-Sil was 9.5 mM min À1 . 27 Silicatein derived from Suberites domuncula, 28 chitin-binding domain (ChBD) found in chitinase A1 from Bacillus circulans WL-1, 29 and the soluble protein tag InaKC 27 derived from Pseudomonas syringae 30 were used to construct fusion proteins.…”

“…Although silicatein is an aggregative enzyme in water, we successfully solved this problem by introducing highly soluble proteins such as ProS2 21 and InaKC. 27 Using this strategy, we constructed a triple-fusion silicatein composed of InaKC, ChBD, and silicatein to fabricate a silica-chitosan hydrogel incorporating horseradish peroxidase (HRP) (Scheme 1). The fusion silicatein, InaKC-ChBD-Sil, was expected to function as an interfacial biocatalyst for silica synthesis on the gel and act as a promising binding molecule between silica and hydrogels.…”

“…34 Previously, our research group successfully demonstrated the fabrication of silica on chitin using ChBD-fused silicatein. 27 In some studies, different tags, such as Cys-tag and GST-tags, have been studied to immobilise silicatein on materials in a milder way. 33,34 However, surface modification is required for these tags.…”

“…Silicatein derived from Suberites domuncula , 28 chitin-binding domain (ChBD) found in chitinase A1 from Bacillus circulans WL-1, 29 and the soluble protein tag InaKC 27 derived from Pseudomonas syringae 30 were used to construct fusion proteins. InaKC was found to be a highly hydrophilic protein, and the fusion silicatein was stably soluble in water after protein refolding (Fig.…”