A Novel Metabolic Pathway for Indole-3-Acetic Acid in Apical Shoots of Populus tremula (L.) x Populus tremuloides (Michx.)

Tuominen, Hannele; Östin, Anders; Sandberg, Göran; Sundberg, Björn

doi:10.1104/pp.106.4.1511

Cited by 72 publications

(40 citation statements)

References 32 publications

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“…3). 14) Treatment OxIAA (5) OxIAA-Glc (6) IAA-Glc (2) IAA-Asp (3) IAA-Glu (4) The low yield of glucosides in the cases of 2-d 2 and 6-d 2 was due to the concomitant reduction of the indoleand 2-oxoindole-ring, respectively, during deprotection of the benzyl groups by catalytic hydrogenation. The yield could be improved by using unprotected glucosyl donors such as O-glucosyl trichloroacetimidate.…”

Section: Resultsmentioning

confidence: 99%

Facile Preparation of Deuterium-Labeled Standards of Indole-3-Acetic Acid (IAA) and Its Metabolites to Quantitatively Analyze the Disposition of Exogenous IAA inArabidopsis thaliana

Kai

Nakamura

Wakasa

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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Section: Resultsmentioning

confidence: 99%

Facile Preparation of Deuterium-Labeled Standards of Indole-3-Acetic Acid (IAA) and Its Metabolites to Quantitatively Analyze the Disposition of Exogenous IAA inArabidopsis thaliana

Kai

Nakamura

Wakasa

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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“…that different conjugates perform different functions in the plant. Certain conjugates, such as indole-3-acetic acidamide, can be intermediates in indole-3-acetic acid destruction (Tuominem et al, 1994). Often indole-3-acetic acid conjugates may serve as reservoirs of inactive indole-3-acetic acid that can be hydrolysed to provide the plant with active hormone.…”

Section: Endogenous Levels Of Free and Conjugated Indole-3-acetic Acimentioning

confidence: 99%

Indole-3-acetic acid metabolism in normal and dwarf micropropagated banana plants (Musa spp. AAA)

Zaffari

Peres

Tcacenco

et al. 2002

Braz. J. Plant Physiol.

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Nanism is one of the most frequent type of mutant in micropropagated banana plants from the Cavendish subgroup. The present study aimed at studying some of the hormone factors involved in this type of mutation. Rhizomes from normal and dwarf plants from the cultivar Grand Naine were incubated for 5 d in the presence of [3 H]-L-tryptophan, [ 3 H]-indole-3-acetic acid and gibberellin, to quantify the endogenous levels of indole-3-acetic acid-ester, indole-3-acetic acid-amide, free indole-3-acetic acid, and cytokinins. The endogenous levels of indole-3-acetic acid and its ester-and amide-conjugated forms were measured in normal and dwarf plants incubated for 30 d with gibberellin, indole-3-acetic acid, and L-and Dtryptophan. In normal plants, the use of [ 3 H]-L-tryptophan resulted in higher levels of radioactivity in the retention times corresponding to indole-3-acetic acid, indole-3-acetic acid-aspartate, indole-3-acetic acid-glycine and indole-3-acetic acid-alanine. These values were higher than those observed in dwarf plants. Higher quantities of radioactive indole-3-acetic acid and of amide-forms in dwarf plants occurred in rhizomes treated with [3 H]-L-tryptophan and gibberellin simultaneously. The endogenous levels of total cytokinins were the same in both materials, while the levels of indole-3-acetic acid in normal plants were 1.5 times higher than in dwarf plants. Moreover, in these ones application of tryptophan and gibberellin coincided in an increase in the levels of free indole-3-acetic acid in dwarf plants and to a decrease in the levels of indole-3-acetic acid-ester and indole-3-acetic acid-amide.Key words: indole-3-acetic acid metabolism, growth regulators, somaclonal variation. Metabolismo do ácido 3-indolil acético em plantas anãs e normais de bananeira micropropagada (Musa spp. AAA):O nanismo em plantas de bananeira micropropagada representa uma das formas mais comuns de alteração genética no subgrupo Cavendish. Utilizaram-se rizomas de plantas normais e anãs de bananeira cv. Grande Naine, incubados durante 5 dias na presença de [ 3 H]-L-triptofano (Trp), [ 3 H]-ácido 3-indolil acético e giberelina (GA 3 ), para o estudo da biossíntese de ácido 3-indolil acético e ácido 3-indolil acético amida e dos níveis endógenos de citocininas totais (Cks) e ácido 3-indolil acético livre. Os teores endógenos de ácido 3-indolil acético e de seus conjugados éster e amida foram obtidos de plantas anãs e normais incubadas durante 30 dias na presença de GA 3, ácido 3-indolil acético e L-e D-triptofano. A aplicação de [ 3 H]-L-triptofano resultou na obtenção de quantidades mais elevadas de radioatividade nas plantas normais, no tempo de retenção coincidente com o de ácido 3-indolil acético, ácido 3-indolil acético-aspartato, ácido 3-indolil acético-glicina e ácido 3-indolil acético-alanina, valores esses superiores aos observados nas plantas anãs. Verificou-se a ocorrência de maior quantidade de ácido 3-indolil acético radioativo e de forma amida nas plantas anãs nos rizomas tratados com [3 H]-L-triptofano e GA ...

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“…Research into the molecular genetic basis for many hormone pathways regulating plant metabolism has indicated the potential for manipulation by genetic transformation (ap Rees 1995;Regan and Rutledge 1995). The transformation of hybrid aspen with two IAA synthesis genes has already demonstrated effects on regulation stem elongation, leaf enlargement, stem diameter growth, xylem formation and axillary bud formation (Tuominen et al 1994(Tuominen et al ,1995. Transgenic sterility has also been suggested as a strategy to reallocate photosynthate £?om r e p r a v e development to stem growth (Strauss et al 1995), although there is little evidence supporting the effectiveness of this strategy.…”

Section: Increasingproductionmentioning

confidence: 99%

Environmental release of transgenic trees in Canada — potential benefits and assessment of biosafety

Mullin¹,

Bertrand²

1998

The Forestry Chronicle

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potential benefits and assessment of biosafety by T. J. Mullinl and S. ~e r t r a n d~The release of new genetic materials into forest ecosystems, La dkploiement de tout mat6riel gknktique nouveau dans les tkosysregardless of the method used to develop them, should be done t5mes forestiers doit se faire d'une manibre skcuritaire et responin an environmentally responsible manner. Canada is participating sable, et ce peu importe la mCthodologie utilisCe pour I'obtenir. with the OECD in efforts to harmonize regulatory control of prodLe Canada participe de concert avec les Pays membres de ucts derived from biotechnology, including forest trees. Prepared I'OCDE Buneffortd'hmonisation de la surveillance ~glementaire under contract to the Canadian ~~~~~t service, the purpose ofthis en biotechnologie, incluant les arbres forestiers. Ce document, prkdocument is to facilitate a discussion within the forestry park pour le Service canadien des for& se veut un outil de disleading to improved direction of research and contributing to the cussion B travers la communautk forestikre. Les questions soulevCes ainsi que les solutions proposkes pourront aider B harmonization of regulatory oversight of genetically engineered mieux dkfinir I'orientation de la recherche forestikre. Cet effort forest trees. While the focus of the paper is on transgenic trees, contribuera I,harmonisation des rbglements concernant les many of the issues raised are to novel arbres forestier-modifiks g&tiquement. Bien que cet article abor. products from tree breeding.de specifiquement le sujet des arbres transgkniques, plusieurs des points soulevLs sont applicables B tout arbre forestier arnkliok gknC-

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A Novel Metabolic Pathway for Indole-3-Acetic Acid in Apical Shoots of Populus tremula (L.) x Populus tremuloides (Michx.)

Abstract: Metabolism of indole-3-acetic acid (IAA) in apical shoots of

Cited by 72 publications

References 32 publications

Facile Preparation of Deuterium-Labeled Standards of Indole-3-Acetic Acid (IAA) and Its Metabolites to Quantitatively Analyze the Disposition of Exogenous IAA inArabidopsis thaliana

Facile Preparation of Deuterium-Labeled Standards of Indole-3-Acetic Acid (IAA) and Its Metabolites to Quantitatively Analyze the Disposition of Exogenous IAA inArabidopsis thaliana

Indole-3-acetic acid metabolism in normal and dwarf micropropagated banana plants (Musa spp. AAA)

Environmental release of transgenic trees in Canada — potential benefits and assessment of biosafety

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