A novel mechanism: Erxian Decoction, a Chinese medicine formula, for relieving menopausal syndrome

Sze, Stephen Cho Wing; Tong, Yao; Zhang, Y.B.; Zhang, Z.J.; Lau, Asy; Wong, Hei-Kiu; Tsang, Kenneth W.; Ng, Tzi Bun

doi:10.1016/j.jep.2009.02.034

Cited by 36 publications

(53 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Modern pharmacological research has revealed that EXD has a comprehensive eff ect on such multiple targets as hypothalamus-pituitary-target gland axis, immune function and free radical metabolism Nian et al, 2006). Our recent study also suggested that EXD treatment would result in increased circulatory estrogen level via the activation of aromatase-related pathway (Sze et al, 2009). The constituents of EXD are numerous and diverse, including alkaloids, phenols, saponins, polysaccharides and oligosaccharides, and many of them have been reported to possess a wide range of bioactivities (Chen et al, , 2008; Kite et al, 2007;Li et al, 2003;Lu et al, 2006;Wang and Lou, 2004;Gong et al, 2003).…”

Section: Introductionmentioning

confidence: 83%

Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography‐mass spectrometry

Wang

Sze

et al. 2009

Biomedical Chromatography

View full text Add to dashboard Cite

A simple and specific LC-DAD-ESI-MS/MS method has been developed and applied for the primary investigation of the chemical constituents absorbed or metabolized in vivo, after the rat oral administration of Erxian Decoction (EXD), a Chinese medicine prescription for menopausal syndromes. Through the online ESI-MS(n) analysis, a total of 35 compounds have been identified or tentatively characterized from the seven tested samples, and 13 of them were unambiguously identified through a direct comparison of the retention time, UV spectra and MS(n) fragmentation patterns with the authentic ones. The results showed that 21 compounds were detected from rat plasma, 20 compounds were detected from rat kidneys and adrenal glands, 19 compounds were detected from rat ovaries, 12 compounds were found in rat intestines, nine compounds were identified from rat livers and nine compounds were detected from rat brains at certain time points after oral administration of the effective EXD fraction.

show abstract

Section: Introductionmentioning

confidence: 83%

Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography‐mass spectrometry

Wang

Sze

et al. 2009

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…Our past studies indicated that EXD relieved the menopausal syndrome by increasing endocrine and antioxidant functions through activation of the aromatase and catalase (CAT) detoxifying pathways. 26 Another study also revealed the action of EXD in prolonging antiosteoporotic effects and the aging of the hypothalamus-pituitary-target gland axis. 37<40 All these research studies supported the clinical application of EXD for treating menopausal symptoms.…”

Section: Discussionmentioning

confidence: 99%

“…Preparation of herbs and methods of quality control had been published in our previous article. 26 For evaluation of consistency of quality among EXD extracts, three batches of EXD extracts were weighed and extracted with 10 mL of methanol in a water bath at 60-C for 15 minutes, followed by ultrasonication for 30 minutes. After centrifugation, the supernatant was filtered by a 0.45-Km Millex A Syringe filter unit and injected in a volume of 10 KL for high-performance liquid chromatography.…”

Section: Interventionmentioning

confidence: 99%

“…After centrifugation, the supernatant was filtered by a 0.45-Km Millex A Syringe filter unit and injected in a volume of 10 KL for high-performance liquid chromatography. 26 The EXD and placebo in this trial were produced by a good manufacturing practice manufacturer (PuraPharm International HK Ltd., Hong Kong SAR; lot no. A090436-01/02; Appendix I, Supplemental Digital Content 1, http://links.lww.com/MENO/A48).…”

Section: Interventionmentioning

confidence: 99%

See 1 more Smart Citation

A randomized, double-blind, controlled trial of a Chinese herbal formula (Er-Xian decoction) for menopausal symptoms in Hong Kong perimenopausal women

Zhong¹,

Tong²,

Tang

et al. 2013

Menopause

View full text Add to dashboard Cite

show abstract

“…Er-Xian Decoction (EXD), a Chinese herbal formula, has been proven to reduce the severity and frequency of hot flushes and ameliorate menopausal symptoms in perimenopausal women without serious adverse events [22]. Moreover, a study based on menopausal rat model revealed that EXD showed an estrogen-like effect in the relief of menopausal syndrome [23, 24]. Naturally, we hypothesized that EXD could have a cardioprotective effect on menopausal animal models.…”

Section: Introductionmentioning

confidence: 99%

The protective effect of Er-Xian decoction against myocardial injury in menopausal rat model

Zhang

Xiang

Zhao

et al. 2018

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundEr-Xian decoction (EXD), a formula of Chinese medicine, is often used to treat menopausal syndrome in China. The aim of the present study was to explore the potential cardioprotective mechanism of EXD against myocardial injury in an ovariectomy-induced menopausal rat model.MethodsWe divided the female Wistar rats into ovariectomy group and sham operation group (SHAM group). The ovariectomized (OVX) rats received treatment of vehicle (OVX group), EXD (EXD group) or 17β-estradiol (E2 group). After 12-week of treatment, the level of estradiol in serum was detected using an electrochemiluminescence immunoassay, and electrophysiologic changes in myocardial action potentials (AP) were evaluated using intracellular microelectrode technique. Changes in the histopathology of the left ventricle and the ultrastructure of the cardiomyocytes were observed by hematoxylin and eosin (HE) staining and transmission electronmicroscopy to assess myocardial injury. Microarrays were applied for the evaluation of gene expression profiles in ventricular muscle of the OVX and EXD rats. Further pathway analyses of the differential expression genes were carried out using the Kyoto Encyclopedia of Genes and Genomes (KEGG). And real-time quantitative RT-PCR (qRT-PCR) was used for verification of the key findings.ResultsThe results from electrophysiological and histomorphological observations demonstrated that EXD had a substantial myocardial protective effect. The EXD-treated rats, in comparison with the OVX rats, demonstrated up-regulated expression of 28 genes yet down-regulated expression of 157 genes in the ventricular muscle. The qRT-PCR assay validated all selected differential expression genes. The KEGG pathway analysis showed that the down-regulated genes were relevant to cardiomyopathy and myocardial contractility. EXD could decrease the mRNA expressions of cardiac myosin (Myh7, Myl2) and integrin (Itgb5) in the ventricular myocardium.ConclusionEXD had a protective effect against myocardial injury in OVX rats, and this cardioprotective effect may be associated with modulation of the expression of cardiac myosin or integrin at the mRNA level.Electronic supplementary materialThe online version of this article (10.1186/s12906-018-2311-9) contains supplementary material, which is available to authorized users.

show abstract

A novel mechanism: Erxian Decoction, a Chinese medicine formula, for relieving menopausal syndrome

Cited by 36 publications

References 34 publications

Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography‐mass spectrometry

Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography‐mass spectrometry

A randomized, double-blind, controlled trial of a Chinese herbal formula (Er-Xian decoction) for menopausal symptoms in Hong Kong perimenopausal women

The protective effect of Er-Xian decoction against myocardial injury in menopausal rat model

Contact Info

Product

Resources

About