A Novel Labeling Reagent of 2-(12-Benzo[b]acridin-5-(12H)-yl)-acetohydrazide for Determination of Saturated and Unsaturated Fatty Acids in Traditional Chinese Herbs by HPLC-APCI-MS

Xie, Yinfeng; Li, Guoliang; You, Jing; Bai, Xinwei; Wang, Chengyan; Zhang, Lin; Zhao, Fuhua; Wu, Xin; Ji, Zhongyin; Sun, Zhi‐Wei

doi:10.1007/s10337-012-2226-4

Cited by 10 publications

(3 citation statements)

References 42 publications

(42 reference statements)

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“…Most UFAs do not naturally absorb in the visible or UV region and the uorescence region, so it is di cult to detect them at trace levels with an absorptiometry. A common method to enhance the sensitivity and selectivity of carboxylic acid detection is the chemical derivatization of the carboxylic acid fraction (Jonnada et al 2017) (Xie et al 2012). However, these reagents have limitations in application such as low detection sensitivity, poor stability, cumbersome analytical procedures, and severe interference with the analysis of biological samples(Toyo'oka 2002).…”

Section: Introductionmentioning

confidence: 99%

New Fluorescence Derivatization Reagent for the Determination of 7 Unsaturated Fatty Acids in Oil Samples by Pre-Column Derivatization HPLC-FLD

You

Shi

et al. 2023

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4-((2-Methyl-1,3-dioxo-2,3-dihydro-1H-benzo[de]isoquinoline-6-yl)amino)-Ethyl-4-Methyl Benzenesulfonate (MNDMB), was designed and synthesized, and it was first successfully applied to label unsaturated fatty acids and HPLC-FLD determination. MNDMB can quickly complete the derivatization reaction with unsaturated fatty acids within 40 min with K2CO3 as the catalyst. The derivatives exhibit high stability and strong fluorescence properties with excitation and emission wavelengths of 285 and 522 nm, respectively. The 7 unsaturated fatty acid derivatives were separated on an Agilent ZORBAX 300SB-C18 column with gradient elution, and good linear correlation coefficients were obtained for all unsaturated fatty acids with correlation coefficients of ≥ 0.9998. The method has low limits of detection (LODs) (4–21 nmol/L) and good precisions ( intraday relative standard deviations [RSDs] were 1.91–3.51%, and interday RSD were 3.33–5.41%). The method was used to analyze two edible oils and successfully detected some unsaturated fatty acids with recoveries ranging from 90.2 to 103.5%. The proposed method has several advantages including specific labeling, simple enrichment operation, and high sensitivity, demonstrating its great utility for monitoring trace unsaturated fatty acids in various complex matrices.

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Section: Introductionmentioning

confidence: 99%

New Fluorescence Derivatization Reagent for the Determination of 7 Unsaturated Fatty Acids in Oil Samples by Pre-Column Derivatization HPLC-FLD

You

Shi

et al. 2023

Preprint

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“…The novel method showed the advantages of the lower detection limits at 1.10 and 1.30 ng mL −1 than the other mentioned methods. In this study, we first selected 2-[12-benzo[b]acridin-5-(12H)-yl]acetohydrazide (BAAH) as the fluorescence labeling reagent (Xie et al, 2012), which could react with triterpenic acids based on a condensation reaction in the presence of water. To overcome this disadvantage, we intended to develop a new HPLC-FLD method using fluorescent labeling in aqueous solution to simultaneously detect multiple triterpenic acids for bioavailability investigation (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

“…To overcome this disadvantage, we intended to develop a new HPLC-FLD method using fluorescent labeling in aqueous solution to simultaneously detect multiple triterpenic acids for bioavailability investigation (Figure 1). In this study, we first selected 2-[12-benzo[b]acridin-5-(12H)-yl]acetohydrazide (BAAH) as the fluorescence labeling reagent (Xie et al, 2012), which could react with triterpenic acids based on a condensation reaction in the presence of water. The amounts of condensing agent and labeling reagent, reaction temperature and time were selected as the main derivatization conditions to be investigated.…”

Section: Introductionmentioning

confidence: 99%

Development of a facile and sensitive HPLC‐FLD method via fluorescence labeling for triterpenic acid bioavailability investigation

You

Zhao

et al. 2017

Biomedical Chromatography

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Triterpenic acids are widely distributed in many fruits and are known for their medicinal benefits. The study of bioavailability has been an important task for a better understanding of the triterpenic acids. Although many methods based on fluorescence labeling for triterpenic acid determination have been established, these reported methods needed anhydrous conditions, which are not suitable for the convenient study of triterpenic acid bioavailability. Inspired by that, a versatile method, which overcomes the difficulty of the reported methods, has been first developed in this study. The novel method using 2-[12-benzo[b]acridin-5- (12H)-yl]-acetohydrazide (BAAH) as the fluorescence labeling reagent coupled with high-performance liquid chromatography with fluorescence detection was first developed for the study of triterpenic acid bioavailability. Furthermore, the labeling conditions have been optimized in order to achieve the best fluorescence labeling yield. Under the optimal conditions, the quantitative linear range of analytes was 2-1000 ng mL , and the correlation coefficients were >0.9998. The detection limits for all triterpenic acid derivatives were achieved within the range of 0.28-0.29 ng mL . The proposed method was successfully applied to the study of triterpenic acid bioavailability with excellent applicability and good reproducibility.

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Polyunsaturated fatty acids from Phyllocaulis boraceiensis mucus block the replication of influenza virus

et al. 2018

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Influenza viruses cause worldwide outbreaks and pandemics in humans and animals every year with considerable morbidity and mortality. The molecular diversity of secondary metabolites extracted from mollusks is a good alternative for the discovery of novel bioactive compounds with unique structures and diverse biological activities. Phyllocaulis boraceiensis is a hermaphroditic slug that exudes mucus, in which was detected hydroxy polyunsaturated fatty acids that exhibited potent antiviral activity against measles virus. The objective of this study was to evaluate this property against Influenza viruses. Cell viability and toxicity of the mucus were evaluated on Madin-Darby canine kidney (MDCK) cells by MTT assay. Antiviral activity from mucus against influenza viruses was carried out by determination of the virus infection dose and by immunofluorescence assays. The crude mucus and its fractions exhibited low cytotoxicity on MDCK cells. A significant inhibition of viral replication, reduced by the order of eight times, was observed in influenza-induced cytopathic effect. In immunofluorescence assay was observed a decrease of more than 80% of the viral load on infected MDCK cell treated with mucus and its fractions. The viral glycoproteins hemagglutinin and neuraminidase located on the surface of the virus are crucial for the replications and infectivity of the influenza virus. Some authors demonstrated that lipids, such as, polyunsaturated fatty acids exhibited multiple roles in antiviral innate and adaptive responses, control of inflammation, and in the development of antiviral therapeutics. As corroborated by other studies, hydroxy polyunsaturated fatty acids interfered with the binding of influenza virus on host cell receptor and reduced viral titers. The results obtained indicated that polyunsaturated fatty acids from P. boraceiensis crude mucus and fractions 39 exerted antiviral activity against influenza virus.

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A Novel Labeling Reagent of 2-(12-Benzo[b]acridin-5-(12H)-yl)-acetohydrazide for Determination of Saturated and Unsaturated Fatty Acids in Traditional Chinese Herbs by HPLC-APCI-MS

Cited by 10 publications

References 42 publications

New Fluorescence Derivatization Reagent for the Determination of 7 Unsaturated Fatty Acids in Oil Samples by Pre-Column Derivatization HPLC-FLD

New Fluorescence Derivatization Reagent for the Determination of 7 Unsaturated Fatty Acids in Oil Samples by Pre-Column Derivatization HPLC-FLD

Development of a facile and sensitive HPLC‐FLD method via fluorescence labeling for triterpenic acid bioavailability investigation

Polyunsaturated fatty acids from Phyllocaulis boraceiensis mucus block the replication of influenza virus

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