A novel label-free electrochemical immunosensor based on the enhanced catalytic currents of oxygen reduction by AuAg hollow nanocrystals for detecting carbohydrate antigen 199

Wang, Rui; Feng, Jiu‐Ju; Liu, Weidong; Jiang, Liu-Ying; Wang, Ai‐Jun

doi:10.1016/j.bios.2017.05.007

Cited by 48 publications

(13 citation statements)

References 57 publications

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“…The limit of detection (LOD, S/N = 3) was calculated to be 0.4 U/mL CA 19-9. As listed in Table S2, a lower LOD was obtained in the developed method compared with that of the reported methods, including photothermal immunoassay (0.83 U/mL), immuno-chromatographic (5.0 U/mL), and CL immunoassay (2 U/mL), and slightly higher with that of the previously reported methods including label-free electrochemical immunosensors with the enhanced catalytic currents (0.23 U/mL, 50 μL) and commercial ELISA kit (0.1 U/mL, 50 μL), but these two methods need large volume of target/sample. Importantly, the LOD obtained is much lower than the cutoff value of 37 U/mL CA 19-9 for diagnosis of pancreatic cancer, which has 69% sensitivity and 85% specificity .…”

Section: Results and Discussionmentioning

confidence: 75%

Electrogenerated Chemiluminescence Immunoassays on Nanoelectrode Ensembles Platform with Magnetic Microbeads for the Determination of Carbohydrate Antigen

Yang

Wei

et al. 2020

Anal. Chem.

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This work reports a gold nanoelectrode ensembles (Au-NEE) platform taken as a disposable electrogenerated chemiluminescence (ECL) platform with immunomagnetic microbeads for ECL immunoassays for the first time. The peak-shaped voltammograms were obtained at the Au-NEE, attributed to the total diffusional overlap. The ECL intensity at Au-NEE was 12.9 folds in the Ru(bpy) 3 2+ -tri-n-propylamine (TPA) ECL system and 19.6 folds in the luminol-H 2 O 2 system, compared with that at the Au macroelectrode using the normalized active area of the electrodes, mainly attributed to the diffusion overlap of the Au-NEE and the edge effect of the individual gold nanodisks of the Au-NEE. The ECL immunoassay on the Au-NEE platform with magnetic microbeads for the determination of cancer biomarkers was developed. Carbohydrate antigen 19-9 (CA 19-9) was chosen as a model analyte while CA 19-9 antibody on the magnetic microbeads was taken as the capture probe, and ruthenium complex-labeled CA 19-9 antibody was used as the signal probe. A "sandwich" bioconjugates on the magnetic beads were transferred onto the ECL platform, and then the ECL measurements were performed in TPA solution. The developed method showed that the ECL peak intensity was directly in proportion to the concentration of CA 19-9 in the range from 0.5 to 20 U/mL with a limit of detection of 0.4 U/mL. This work demonstrates that the Au-NEE can be employed as a useful disposable ECL platform with the merits of cheapness, low nonspecific adsorption and practical application. The proposed approach will open a new avenue in the point-of-care test for the determination of protein biomarkers.

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Section: Results and Discussionmentioning

confidence: 75%

Electrogenerated Chemiluminescence Immunoassays on Nanoelectrode Ensembles Platform with Magnetic Microbeads for the Determination of Carbohydrate Antigen

Yang

Wei

et al. 2020

Anal. Chem.

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“…152,153 To date, researchers have developed a variety of HMNM-based electrochemical, FL and colorimetric biosensors to detect disease-related protein markers, such as tumor, hepatic, cardiac, inflammatory markers, and others (Table 2). 24,30,35,36,46,47,49–52,64,65,81,84,85,88,89,98,101–103,107–110,114,122,154–178…”

Section: Applications Of Hmnms In Analytical Chemistrymentioning

confidence: 99%

Heterometallic nanomaterials: activity modulation, sensing, imaging and therapy

Wang

Yuan

et al. 2022

Chem. Sci.

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“…11 Advanced nanomaterials have a significant role in the development of electrochemical immunosensors. 12 In this regard, wrinkled fibrous nano-silica (WFNS) have attracted the attention of electrochemical immunoassay devices. 13 WFNS nanomaterials have been noted for their easy synthesis, adjustable particle size, large pore volume, excellent mechanical stability, low toxicity, complete biocompatibility, adjustable pore size, and unique large surfaces.…”

Section: Introductionmentioning

confidence: 99%

“…Advanced nanomaterials have a significant role in the development of electrochemical immunosensors 12 . In this regard, wrinkled fibrous nano‐silica (WFNS) have attracted the attention of electrochemical immunoassay devices 13 .…”

Section: Introductionmentioning

confidence: 99%

Sensitive recognition of prostate‐specific antigen using biotinylated antibody encapsulated on D‐penicillamine decorated wrinkled silicate nanoparticles (WSN): An innovative sandwich‐type biosensor toward diagnosis of prostate cancer

Behyar

Farshchi

Hasanzadeh

2022

J of Molecular Recognition

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In this study, a new sandwich-type biosensor was developed to specific recognition of prostate-specific antigen (PSA) and early-stage diagnosis of prostate cancer using encapsulation of biotinylated antibody (Ab1) of prostate-specific antigen on D-penicillamine decorated wrinkled silicate nanoparticles (WSN). For the first time, KCC-1-NH-DPA was synthesized and used to immobilization of biomacromolecules. So, fabricated immunosensor was performed by on sandwich-type strategy. After fabrication of immunosensor, cyclic voltammetry, differential pulse voltammetry, and square wave voltammetry techniques were used to electrochemical evaluation of immune-platform for PSA detection. The proposed biocompatibility immune-platform provided a novel interface toward sensitive bioanalysis of PSA biomarker in human plasma samples. Due to the use of gold nanoparticles functionalized Cysteamine (Cys A) in the structure of the secondary antibody (Ab2 [HRP-Ab2]), the intensity of the electrochemical signal has increased, resulting in a more accurate detection of the target molecules. Under the right conditions, the engineering immunosensor provides good bioanalytical performance for determining the PSA biomarker in the linear range of 0.002 to 60 μg L À1 which low limit of quantification was 0.002 μg L À1 . As a result, it is suggested to use these immune-devices in the clinical pre-diagnosis of prostate cancer.

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A novel label-free electrochemical immunosensor based on the enhanced catalytic currents of oxygen reduction by AuAg hollow nanocrystals for detecting carbohydrate antigen 199

Cited by 48 publications

References 57 publications

Electrogenerated Chemiluminescence Immunoassays on Nanoelectrode Ensembles Platform with Magnetic Microbeads for the Determination of Carbohydrate Antigen

Electrogenerated Chemiluminescence Immunoassays on Nanoelectrode Ensembles Platform with Magnetic Microbeads for the Determination of Carbohydrate Antigen

Heterometallic nanomaterials: activity modulation, sensing, imaging and therapy

Sensitive recognition of prostate‐specific antigen using biotinylated antibody encapsulated on D‐penicillamine decorated wrinkled silicate nanoparticles (WSN): An innovative sandwich‐type biosensor toward diagnosis of prostate cancer

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