A Novel Interaction Mode between Acrylamide and its Specific Antibody

Zhou, Shuang; Wang, Dan; Zhang, Chen; Zhao, Yunfeng; Zhao, Meiping; Wu, Yongning

doi:10.1080/15321819.2014.947432

Cited by 3 publications

(2 citation statements)

References 54 publications

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“…AA is strongly polar and has an 84.5°C melting point and mild interactions with other organic compounds. [ 1 ] AA is produced in the reaction of proteins called Maillard reactions between the amino acids (especially asparagine) and sugar reduction. [ 2 ] The Maillard reaction mechanism is responsible for the creation of high‐starch food during cooking at high temperatures.…”

Section: Introductionmentioning

confidence: 99%

A molecular biomimetic sensor of tris(2‐benzimidazolylmethyl)amine‐based iron(III) complex for acrylamide detection: Electrochemical study and DFT calculations

Mersal

Hessien

Jouaid

et al. 2021

J Chinese Chemical Soc

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A new biomimetic sensor (BS) has been prepared by modifying the carbon paste electrode (CPE) based on [Fe(NTB)Cl2]Cl·EtOH (FeC) {NTB = tris(2‐benzimidazolylmethyl)amine} for the electrochemical detection of acrylamide (AA). The development of the reported BS was focused on the creation of an AA with α‐NH2 group of N‐terminal valine of hemoglobin (HG), which reduces the peak current of HG‐Fe+3 reduction. The fabricated BS was characterized by scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV), as well as square wave voltammetry (SWV) for studying the electrochemical properties of AA on its surface. Under optimized conditions, the BS was applied for the direct determination of AA in two linear concentration ranges: 1.0 × 10−6 and 1.0 × 10−4 M. The first linear was observed from 1.0 × 10−6 to 1 × 10−5 M with a limit of detection (LOD) of 1.465 × 10−7 M, while the second linear part was detected from 1.0 × 10−5 to 1 × 10−4 M with LOD of 5.67 × 10−6 M. Finally, the constructed BS was successfully applied for the evaluation of AA in various potato chips, and the results were comparable to those obtained from other electrochemical methods. The density functional theory (DFT) could successfully explain the recognition process of acrylamide, that is, the hydroamination of the alkene group of acrylamide by imidazole moiety of the iron complex.

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Section: Introductionmentioning

confidence: 99%

A molecular biomimetic sensor of tris(2‐benzimidazolylmethyl)amine‐based iron(III) complex for acrylamide detection: Electrochemical study and DFT calculations

Mersal

Hessien

Jouaid

et al. 2021

J Chinese Chemical Soc

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“…There are alternative analytical techniques such as enzyme-linked immunosorbent assay (ELISA) kits that are able to detect AA after sample clean-up using solid phase extraction (SPE), 10 but covalent alkylation takes place during the immunoreaction of AA with its specific antibody mainly through the cysteine and histidine from the surface of the antibody. 11 Biosensors based on detection mechanisms that mimic toxicological mechanism in vitro for detection of AA have been reported: DNA 12 or haemoglobin 13 form adducts with AA and change their electrochemical properties. However, the formed adducts are irreversible, which limits the biosensor design to disposable devices.…”

Section: Introductionmentioning

confidence: 99%

Simple, selective and fast detection of acrylamide based on glutathione S-transferase

Bucur

Radu

2018

RSC Adv.

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Acrylamide (AA) is a toxic compound formed in thermally prepared foods by Maillard reaction. Besides foods, AA may be found in cosmetic products as an impurity of the widely-used non-toxic polyacrylamide. We present a novel, fast and selective detection method based on the amperometric monitoring of the coupling reaction between reduced glutathione (GSH) and AA catalyzed by glutathione S-transferase (GST) to produce an electrochemically inactive compound. We have used electrodes modified with cobalt-phthalocyanine to monitor the decrease of GHS concentration at +300 mV. Our system is simple, does not require supplementary substrates such as 1-chloro-2,4-dinitrobenzene (CDNB) nor have disadvantageous competitive kinetics characteristic to inhibition like signals. Using the optimum concentration of 100 mM GSH we have obtained a linear calibration graph from 7 to 50 mM AA and a limit of detection of 5 mM AA. The method is not affected by interfering compounds usually found in foods and was applied for real sample analysis.

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Use of 2-Naphthalenethiol for Derivatization and Determination of Acrylamide in Potato Crisps by High-Performance Liquid Chromatographic with Fluorescence Detection

et al. 2018

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A Novel Interaction Mode between Acrylamide and its Specific Antibody

Cited by 3 publications

References 54 publications

A molecular biomimetic sensor of tris(2‐benzimidazolylmethyl)amine‐based iron(III) complex for acrylamide detection: Electrochemical study and DFT calculations

A molecular biomimetic sensor of tris(2‐benzimidazolylmethyl)amine‐based iron(III) complex for acrylamide detection: Electrochemical study and DFT calculations

Simple, selective and fast detection of acrylamide based on glutathione S-transferase

Use of 2-Naphthalenethiol for Derivatization and Determination of Acrylamide in Potato Crisps by High-Performance Liquid Chromatographic with Fluorescence Detection

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