A novel immune-tolerable and permeable lectin-like protein from mushroom Agaricus bisporus

Ismaya, Wangsa T.; Yunita,; Efthyani, Alida; Lai, Xuelei; Retnoningrum, Debbie Soefie; Rachmawati, Heni; Dijkstra, Bauke W.; Tjandrawinata, Raymond R.

doi:10.1016/j.bbrc.2016.04.020

Cited by 17 publications

(27 citation statements)

References 15 publications

(29 reference statements)

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“…Hence, this limits the throughput and usefulness, especially for permeation enhancer fast screening. Permeability assay was carried out within the first hour after the intestine was collected to ensure that the cells had viability and activity [ 17 ]. The results from this study showed that all SODs except SOD_Cl were transported across the non-everted rat intestine.…”

Section: Discussionmentioning

confidence: 99%

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Gliadin Peptide Facilitates FITC Dextran Transport across the Non Everted Gut Sac of Rat Small Intestine

et al. 2018

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Superoxide dismutase (SOD) is an antioxidant protein. When administered orally, it has low bioavailability due to its low permeation. In a previous study we fused gliadin peptide P51 (LGQQQPFPPQQPYPQPQPF) and gliadin peptide P61 (QQPYPQPQPF) with SOD Citrus limon (SOD_Cl), namely GliSOD_P51 and GliSOD_P61 to increase permeation of SOD_Cl through intestine. In this work, the permeation of fluorescein isothiocyanate (FITC)-Dextran 10 kDa, FD10 and 40 kDa, FD40 as paracellular transport markers across excised rat intestinal wall was investigated with the presence of GliSOD_P51 and GliSOD_P61. A permeability study was performed using non-everted rat intestine by incubating FD10 or FD40 with SOD_Cl, and GliSOD_P61. The presence of SOD_Cl, GliSOD_P51 or GliSOD_P61 inside intestine (apical) and outside intestine (basolateral) was analyzed by protein electrophoresis. The concentration of FD that penetrated to the basolateral solution was analyzed by spectrofluorometry. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed the presence of GliSOD_P51 and GliSOD_P61 but not SOD_Cl in basolateral compartment. The percentage of FD10 but not FD40 and SOD_Cl that penetrated to the basolateral solution significantly increased with the presence of gliadin in GliSOD_P51 and GliSOD_P61. GliSOD_P51 and GliSOD_P61 are able to penetrate the rat intestinal epithelial membrane and the gliadin peptides facilitate FD10 to penetrate the epithelial.

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Section: Discussionmentioning

confidence: 99%

“…The ability of recombinant proteins crossing intestine walls was determined using the non-everted gut sac method followed by the published methods with slight modification [ 17 ]. The non-everted gut sac method involves placing the sample solution in the rat gut sac without everting.…”

Section: Methodsmentioning

confidence: 99%

Gliadin Peptide Facilitates FITC Dextran Transport across the Non Everted Gut Sac of Rat Small Intestine

et al. 2018

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“…To date, only few proteins from A. bisporus have been investigated experimentally using an active fraction isolated from the fruiting bodies or as a recombinant protein. They are ABL [5], Abmb [20], PPO1 and PPO2 [21], PPO3 [4,22], PPO4 [23], and the most recently reported AB21 [24]. The latter is stable at a wide pH range (4.5-10.5), is thermostable, and has an affinity to transition metal ions, but displays no lectin activity.…”

Section: Introductionmentioning

confidence: 95%

Lectins from the Edible Mushroom Agaricus bisporus and Their Therapeutic Potentials

Ismaya¹,

Tjandrawinata²,

Rachmawati

2020

Molecules

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The mushroom Agaricus bisporus secretes biologically active compounds and proteins with benefits for human health. Most reported proteins from A. bisporus are tyrosinases and lectins. Lectins are of therapeutic or pharmaceutical interest. To date, only limited information is available on A. bisporus lectins and lectin-like proteins. No therapeutic products derived from A. bisporus lectin (ABL) are available on the market despite its extensive exploration. Recently, A. bisporus mannose-binding protein (Abmb) was discovered. Its discovery enriches the information and increases the interest in proteins with therapeutic potential from this mushroom. Furthermore, the A. bisporus genome reveals the possible occurrence of other lectins in this mushroom that may also have therapeutic potential. Most of these putative lectins belong to the same lectin groups as ABL and Abmb. Their relationship is discussed. Particular attention is addressed to ABL and Abmb, which have been explored for their potential in medicinal or pharmaceutical applications. ABL and Abmb have anti-proliferative activities toward cancer cells and a stimulatory effect on the immune system. Possible scenarios for their use in therapy and modification are also presented.

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“…Safety evaluation of a protein prior to its development for pharmaceutical or medicinal purposes is important, to ensure its efficacy and to prevent negative impact such as anaphylaxis or auto‐immune responses upon used . Previously, LSMT was demonstrated to be tolerated by the outbred Swiss Webster mice immune system upon weekly administration at 25 μg in mice up to 4 weeks . Although this animal model is commonly used for toxicity study during drug development, its sensitivity may not be sufficient for immunological study .…”

Section: Discussionmentioning

confidence: 99%

Biological responses in Balb/c mice after long‐term parenteral administration of the light subunit of mushroom tyrosinase

Ismaya¹,

Efthyani

Tjandrawinata³

et al. 2017

J Biochem & Molecular Tox

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Light subunit of mushroom tyrosinase (LSMT) is a protein of unknown function from mushroom Agaricus bisporus that has been demonstrated to permeate through rat intestine ex vivo. Thus, it can be absorbed in the intestine, thereby holding a promise as a drug carrier for oral administration, similar to HA-33 protein from botulinum, one of the closest structural homologs of LSMT. However, the safety of LSMT should be ensured prior to its use. Here, we described biological response of LSMT upon weekly intraperitoneal administration of 50 μg/day to the Balb/c mice for 12 weeks. Motoric and behavior profiles, as well as the index of main organs (liver, spleen, lung, heart, and kidney), and body weight, were not significantly changed as compared with the control group. Also, no IgG was detected in the serum. The results suggest that LSMT is safe for further development.

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A novel immune-tolerable and permeable lectin-like protein from mushroom Agaricus bisporus

Cited by 17 publications

References 15 publications

Gliadin Peptide Facilitates FITC Dextran Transport across the Non Everted Gut Sac of Rat Small Intestine

Gliadin Peptide Facilitates FITC Dextran Transport across the Non Everted Gut Sac of Rat Small Intestine

Lectins from the Edible Mushroom Agaricus bisporus and Their Therapeutic Potentials

Biological responses in Balb/c mice after long‐term parenteral administration of the light subunit of mushroom tyrosinase

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